Method for detecting triglyceride ingredient in fish oil on line by high performance liquid chromatography

A high-performance liquid chromatography and fish oil triglyceride technology, applied in the field of analytical chemistry, can solve the problems of high difficulty in product online quality monitoring, high technical requirements for experimenters, and high cost of mass spectrometry technology, and achieve low equipment configuration requirements, analysis and detection Low cost, good separation effect

Active Publication Date: 2018-06-19
GUANGZHOU HANFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results of literature survey show that there are few studies on triglycerides in fish oil under conventional liquid chromatography conditions. High, complicated operation, and high technical requirements for experimenters, it is difficult to realize online quality monitoring of products

Method used

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  • Method for detecting triglyceride ingredient in fish oil on line by high performance liquid chromatography
  • Method for detecting triglyceride ingredient in fish oil on line by high performance liquid chromatography
  • Method for detecting triglyceride ingredient in fish oil on line by high performance liquid chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: This example compares the literature chromatographic conditions with the patent chromatographic conditions

[0028] Literature (Hao Suli, Liu Jinxiu, Xu Kangsen. Determination of structured triglyceride content in structured fat emulsion injection by ELSD method [J]. Journal of Pharmaceutical Analysis, 2004,24(03):301-303.). Literature chromatographic conditions: chromatographic column is Kromasil100-5C 18(250mm×4.6mm, 5μm); the mobile phase is a mixed mobile phase system, in which mobile phase A is acetonitrile, mobile phase B is acetonitrile-acetone-isooctane (29.5:48.5:22.0), using gradient elution, the situation is as follows : 0-2min (A: 100%-60%, B: 0%-40%), 2-22min (A: 60%-35%, B: 40%-65%), 22-42min (A: 35%) %-25%, B: 65%-75%), 42-50min (A: 25%-10%, B: 75%-90%), 50-52min (A: 10%-100%, B: 90 %-0%), 52-60min (A: 100%, B: 0%), the flow rate of the mobile phase is 1.0mL / min, and the column temperature is 25°C; 2.6mL / min.

[0029] Chromatographic condit...

Embodiment 2

[0038] Example 2: Liquid Chromatographic Analysis of Triglyceride Components in Fish Oil Samples at Different Process Stages

[0039] Take deep-sea fish oil raw materials (tuna fish oil and anchovy fish oil), 0.1 g of refined fish oil intermediate product and refined fish oil finished product respectively, weigh them accurately, put them in a 10 mL measuring bottle, add n-hexane to dissolve and constant volume, and prepare the The test solution was filtered through a 0.45μm filter membrane for determination by high performance liquid chromatography.

[0040] Get above-mentioned each need testing solution 5 μ L, inject liquid chromatograph, measure by the discoloration condition of this patent in embodiment 1, record chromatogram.

[0041] From the chromatographic analysis results of two fish oil raw materials, refined fish oil intermediates and refined fish oil products, it can be known that figure 2 , 3 As shown, the triglyceride information of fish oil raw materials of di...

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Abstract

The invention discloses a method for detecting a triglyceride ingredient in fish oil on line by high performance liquid chromatography. The method comprises the following steps: (1) dissolving a fishoil sample, carrying out constant volume to a scale, and evenly shaking to obtain a sample solution for testing; (2) carrying out high performance liquid chromatography analysis on the fish oil samplein the step (1), adopting an evaporative light-scattering detector to measure ingredients of the triglyceride in the fish oil sample, carrying out qualitative analysis and segmented quantification onthe triglyceride ingredient in the fish oil, and comparing the content change difference of the triglyceride ingredient in fish oil samples of different production stages to carry out online qualitymonitoring on the production process of refined fish oil. The method has the advantages of being simple in preparation, economic, environmentally friendly and high in safety, and can be used as the online quality control auxiliary means of a refined fish oil product production technology.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for on-line detection of triglyceride components in fish oil by high performance liquid chromatography. Background technique [0002] Fish oil is the general term for all oily substances in fish, including body oil, liver oil and brain oil. Unlike other animal fats or vegetable oils, fish oil is rich in n-3 unsaturated fatty acids, among which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the most studied. The unsaturated fatty acid composition of human beings is also an essential nutrient for human growth and development. Studies have shown that fish oil has the effects of lowering blood lipids, softening blood vessels, supplementing brain nutrition, and assisting in improving memory. Therefore, the healthy dietary supplements, health care products and medicines, which use fish oil as raw material, have been processed and refined through ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74G01N30/88
CPCG01N30/02G01N30/06G01N30/74G01N30/88G01N2030/027G01N2030/062G01N2030/8813G01N2030/884
Inventor 王小妹康丹瑜王国财梁北梅唐顺之关伟键许文东袁诚
Owner GUANGZHOU HANFANG PHARMA
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