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Culture method for needle mushroom liquid strains

A technology of liquid strains and culture methods, which is applied in the field of cultivation of Flammulina velutipes liquid strains, can solve problems such as less consideration of the fineness of liquid strains, and achieve the effects of strong activity, high biological efficiency, and excellent performance

Active Publication Date: 2018-06-22
陕西众兴高科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Liquid culture has many advantages such as short cycle, high output, less floor space, easy manipulation, stable production conditions, etc. Although there are some reports on the liquid culture technology of Flammulina velutipes strains, most of them are investigated around the growth state of mycelium. Less consideration is given to the excellent performance of the obtained liquid strains, and there are fewer researches on improving the overall production performance of Flammulina velutipes through liquid strain cultivation

Method used

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  • Culture method for needle mushroom liquid strains
  • Culture method for needle mushroom liquid strains
  • Culture method for needle mushroom liquid strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Provide the cultivation method of Flammulina velutipes liquid strain, following steps:

[0028] (1) For the cultivation of strains in shake flasks, inoculate the mother species after activation on the slant into liquid medium, place them in a constant temperature incubator at 26°C for shaking culture, the speed of the shaker is 160r / min, and the ventilation rate is: 1mL of liquid medium is fed with 0.2mL of gas, and cultivated for 4d to obtain the shake flask bacterial classification; After culturing for 1 day, add the first additive of 0.05 times the total weight of the liquid medium added in the shake flask to the liquid medium; After 2 days, add the second additive of 0.10 times the total weight of the liquid medium added in the shake flask to the liquid medium;

[0029] (2) fermentor cultivation, the shake flask bacterial classification prepared by step (1) is inoculated to the fermentor that liquid medium is housed according to the volume ratio inoculum of 15% and ...

Embodiment 2

[0036] Provide the cultivation method of Flammulina velutipes liquid strain, following steps:

[0037] (1) For the cultivation of strains in shake flasks, inoculate the mother species after activation on the slant into liquid medium, and place them in a constant temperature incubator at 24°C for shaking culture. 1mL of liquid medium is fed with 0.3mL of gas, and cultivated for 3d to obtain the shake flask bacterial classification; After culturing for 1 day, add the first additive of 0.08 times the total weight of the liquid medium added in the shake flask to the liquid medium; After 2 days, add the second additive of 0.17 times the total weight of the liquid medium added in the shake flask to the liquid medium;

[0038](2) fermentor culture, get the shake flask strain that step (1) prepares to inoculate to the fermentor that liquid medium is housed according to 10% volume ratio inoculum and carry out fermentation culture, guarantee that Ph value is 4.5-7.5, culture temperature...

Embodiment 3

[0046] Provide the culture method of Flammulina velutipes liquid strain, differ from Example 2 in that, further limit, the preparation method of described peanut shell enzymatic hydrolysis solution is as follows: take peanut shell and wash and dry, add after crushing to peanut shell weight 20 times the wolfberry In the aqueous solution of sodium hydrochloride, heat up to 80°C and stir for 1 hour, then cool down to 45°C to obtain a mixed solution, add an equal weight of cellulase and trypsin mixture to the mixed solution, stir at 45°C for 12 hours, and then centrifuge to collect the supernatant, namely Obtain the peanut shell enzymatic hydrolysis solution.

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Abstract

The invention provides a culture method for needle mushroom liquid strains. The method comprises the steps of shake flask strain culture and fermentation tank culture. An additive is reasonably addedinto a liquid culture medium in the culture process, the obtained liquid strains are good in quality and high in yield, nutrient substances can be better absorbed during solid culture, and the obtained needle mushroom sporocarp is high in protein content, good in nutritive value and high in economic benefits.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, in particular to a method for cultivating liquid strains of Flammulina velutipes. Background technique [0002] Flammulina velutipes is not only a delicious food, but also a good health food. It is rich in B vitamins, vitamin C, carbohydrates, minerals, carotene, various amino acids, phytohemagglutinin, polysaccharides, taurine, mushrooms Purine, ophiopogon sterol, cytolytic toxin, mushroom cytotoxin, etc. have a good effect on enhancing intelligence, especially children's height and intellectual development, and are called "wisdom-enhancing mushrooms" abroad. Flammulina velutipes has high medicinal value. Regular use of Flammulina velutipes can enhance the body’s resistance to cancer cells. Regular consumption of Flammulina velutipes can also lower cholesterol, prevent liver diseases and gastrointestinal ulcers, enhance the body’s righteousness, and prevent diseases and keep fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40
Inventor 肖林刚李银娟杨香院李坤
Owner 陕西众兴高科生物科技有限公司