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Method for measuring chitosan oligosaccharide content by ultraviolet visible spectrophotometry using water-soluble aniline blue as probe

A spectrophotometry, chitosan oligosaccharide technology, applied in the measurement of color/spectral characteristics, etc., can solve the problem of immature chitosan oligosaccharide content determination method, and achieve the effects of cheap reagents, good reproducibility and high sensitivity

Inactive Publication Date: 2018-06-22
GUANGDONG PHARMA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2014, the Health and Family Planning Commission listed oligochitosan as a food additive, and fertilizers and health products with oligochitosan as the main component were introduced continuously. However, the determination method of oligochitosan content is not yet mature. Therefore, a simple and effective determination New method for chitosan oligosaccharide content has important implications
At present, at home and abroad, there are few related studies on the direct determination of chitosan oligosaccharide content by UV-visible spectrophotometry.

Method used

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  • Method for measuring chitosan oligosaccharide content by ultraviolet visible spectrophotometry using water-soluble aniline blue as probe
  • Method for measuring chitosan oligosaccharide content by ultraviolet visible spectrophotometry using water-soluble aniline blue as probe
  • Method for measuring chitosan oligosaccharide content by ultraviolet visible spectrophotometry using water-soluble aniline blue as probe

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Embodiment

[0062] 1. a kind of method taking water-soluble aniline blue as the ultraviolet-visible spectrophotometry of probe to measure chitosan oligosaccharide content, it may further comprise the steps:

[0063] 1) draw the standard curve of chitosan oligosaccharide (COS)

[0064] Add 2.00 mL of citric acid-hydrochloric acid-sodium hydroxide buffer solution of a certain pH 2.0, water-soluble aniline blue (1.0×10 -4 mol / L) 1.50mL, a certain concentration volume gradient COS standard solution (20.00μg / mL) was made to volume with distilled water and shaken well to prepare a standard solution with a gradient concentration, which was heated in a water bath at 80°C for 10 minutes, and the reaction After cooling for 30 minutes, measure the absorbance ABS at a wavelength of 590nm with water as a reference on a UV spectrophotometer; where the reagent blank is marked as A 0 , the solution containing chitosan oligosaccharide is recorded as A, and calculates △A=A–A 0 , to establish a standard c...

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Abstract

The invention relates to a method for measuring chitosan oligosaccharide content by ultraviolet visible spectrophotometry using water-soluble aniline blue as a probe, and belongs to the field of macromolecular detection. According to the invention, chitosan oligosaccharide is found to be associated with water-soluble aniline blue in a citric acid-hydrochloric acid-sodium hydroxide buffer solution;in a certain concentration range, the absorbance value of the chitosan oligosaccharide decreases with the increase of the chitosan oligosaccharide content; in a certain concentration range, the decreased value of the absorbance and the concentration of chitosan oligosaccharide have a linear relationship; and a quantitative analysis method of the chitosan oligosaccharide is established by taking the linear relationship as a quantitative basis of the chitosan oligosaccharide. The method needs to consider whether the working curve of an actual sample is similar to the working curve of a standardproduct, has the advantages of cheap reagent, high sensitivity, good reproducibility, simple and convenient operation and the like, and is suitable for popularization and use in practical application.

Description

technical field [0001] The invention relates to a method for measuring chitosan oligosaccharide content by ultraviolet-visible spectrophotometry using water-soluble aniline blue as a probe, belonging to the field of macromolecular detection. Background technique [0002] Chitosan oligosaccharide (COS) is extracted from the chitin of the biological shell of shrimps and crabs or obtained by enzymatic hydrolysis reaction with chitosan oligosaccharide as raw material. The low-molecular-weight glucose formed by linking has good water solubility, is easily absorbed by the human body and has high biological activity. Chitosan oligosaccharides retain the functions of chitosan oligosaccharides to lower blood pressure, lower cholesterol, treat burns, discharge toxic and harmful substances in the body, resist oxidation, inhibit bacteria, resist tumors, enhance immunity, and strengthen the functions of liver and other organs, which has attracted the attention of medical researchers. Cl...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 白研苏政权
Owner GUANGDONG PHARMA UNIV
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