Preparation method of a natural polymer-based 3D porous composite scaffold with controllable pore size

A technology of natural macromolecules and composite scaffolds, applied in the field of preparation of natural macromolecular-based 3D porous composite scaffolds, can solve the problems of limited amino group content of active groups, etc., and achieves the enhancement of hydrophobicity, the expansion of emulsion concentration range, and the saving of raw materials. Effect

Active Publication Date: 2020-11-17
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, gelatin is insoluble in cold water, and forms a thermally reversible gel when the temperature is below 35 °C, and the reactive active group amino group content of gelatin is limited

Method used

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  • Preparation method of a natural polymer-based 3D porous composite scaffold with controllable pore size
  • Preparation method of a natural polymer-based 3D porous composite scaffold with controllable pore size
  • Preparation method of a natural polymer-based 3D porous composite scaffold with controllable pore size

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Preparation of aminated gelatin: Dissolve 2 g of B-type gelatin in 50 ml, pH5.0, 0.1 M phosphate buffer solution, then add 6.4 g of ethylenediamine and 1.222 g of EDC, and adjust the pH to 5.0 and dilute to 100 ml with phosphate buffer solution, stir and react at 37°C for 1 h, then pour it into a dialysis bag for dialysis for 3 days, change the water 2 to 3 times a day, and finally freeze-dry in a low-temperature refrigerator to obtain a white product;

[0035] (2) Preparation of aminated gelatin nanoparticles: Dissolve 1.25 g of modified gelatin in 25 ml of distilled water, then add 25 ml of desolvating reagent acetone, redissolve the upper white suspension in 25 ml of distilled water and adjust the pH When the value is 3, add acetone dropwise until a white precipitate is formed, then add 150 μl glutaraldehyde for cross-linking reaction for 3 h. Finally, use a centrifuge to centrifuge the reacted mixed solution at 12500 g for 30 min, redissolve the centrifuged lowe...

Embodiment 2

[0039] (1) Preparation of aminated gelatin: Dissolve 5 g of type B gelatin in 125 ml, pH 5.0, 0.1 M phosphate buffer solution, then add 16 g of ethylenediamine and 3.055 g of EDC, and adjust the pH to 5.0 and dilute to 250 ml with phosphate buffer solution, stir and react at 37°C for 1 h, then pour it into a dialysis bag for dialysis for 3 days, change the water 2 to 3 times a day, and finally freeze-dry in a low-temperature refrigerator to obtain a white product;

[0040] (2) Preparation of aminated gelatin nanoparticles: Dissolve 2.5 g of aminated gelatin in 50 ml of distilled water, then add 50 ml of desolvating reagent acetone, redissolve the upper white suspension in 50 ml of distilled water and adjust the pH When the value is 3, add acetone dropwise until a white precipitate is formed, and then add 300 μl glutaraldehyde for cross-linking reaction for 3 h. Finally, use a centrifuge to centrifuge the reacted mixed solution at 12500 g for 30 min, redissolve the centrifuged ...

Embodiment 3

[0044] (1) Preparation of aminated gelatin: Dissolve 10 g of type B gelatin in 250 ml, pH5.0, 0.1 M phosphate buffer solution, then add 32 g of ethylenediamine and 6.11 g of EDC, and adjust the pH to 5.0 and dilute to 500 ml with phosphate buffer solution, stir and react at 37°C for 1 h, then pour into a dialysis bag for dialysis for 3 days, change the water 2 to 3 times a day, and finally freeze-dry in a low-temperature refrigerator to obtain a white product;

[0045] (2) Preparation of aminated gelatin nanoparticles: Dissolve 2.5 g of aminated gelatin in 50 ml of distilled water, then add 50 ml of desolvating reagent acetone, redissolve the upper white suspension in 50 ml of distilled water and adjust the pH When the value is 3, add acetone dropwise until a white precipitate is formed, and then add 300 μl glutaraldehyde for cross-linking reaction for 3 h. Finally, use a centrifuge to centrifuge the reacted mixed solution at 12500 g for 30 min, redissolve the centrifuged lowe...

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Abstract

The invention provides a method for preparing a natural polymer-based 3D porous composite scaffold with a controlled pore size. The method includes first preparing aminated gelatin, preparing aminatedgelatin nanoparticles by combination with a twice desolvation method, using the particles as a stabilizer for Pickering high internal phase emulsion, dissolving the aminated gelatin and dialdehyde starch into deionized water to form a continuous phase of an emulsion, taking an organic solvent as a dispersed phase, performing homogeneous emulsification treatment to form an oil-in-water Pickering high internal phase emulsion, and then through the intermolecular covalent crosslinking reaction; fixing the continuous phase of the emulsion, and finally removing the dispersed phase through solvent evaporation to obtain the 3D porous composite scaffold with the controlled pore size. According to the method, a Pickering emulsion template method is adopted, the aminated gelatin nanoparticles are taken as the stabilizer, a natural polymer is taken as a base material, and the problems that at present, the porous scaffold is uncontrollable, the material is difficult to degrade, and a degradation product poisons tissue cells are solved. The porous scaffold prepared by the method has good application prospects in the field of tissue engineering.

Description

technical field [0001] The invention relates to the field of biomedical tissue engineering composite materials, in particular to a method for preparing a natural polymer-based 3D porous composite scaffold with controllable aperture. Background technique [0002] The proposal and development of regenerative medicine and tissue engineering have brought new hope for the repair and treatment of human damaged tissues clinically. Due to the fact that damaged tissue cells do not have the ability to grow independently in three-dimensional space, it is difficult to form specific tissues. Therefore, it is necessary to use tissue engineering scaffolds as a temporary support framework system in damaged parts of the human body to maintain cell adhesion, migration and differentiation. In practical clinical applications, ideal cell scaffold materials should have good biocompatibility, have no toxicity to organisms, and will not cause inflammation or teratogenic reactions. The material sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/48A61L27/56A61L27/58C08B31/18C08H1/00
CPCA61L27/48A61L27/56A61L27/58A61L2400/12C08B31/18C08H1/00C08L89/00C08L3/10
Inventor 林炜薛万波王春华谷新林
Owner SICHUAN UNIV
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