Animal collagen polypeptide extraction method

A technology of collagen polypeptide and extraction method, which is applied in the field of food chemical industry, can solve problems such as deep color, affecting digestibility, strong fishy smell, etc., and achieve the effect of simple process, excellent product quality and good chemical properties

Inactive Publication Date: 2018-06-26
刘明明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1896262A discloses "technology for producing animal high-calcium powder, chondroitin, and collagen by compound enzyme method". There are the following disadvantages in protein powder or further made into collagen polypeptide: (1) has a strong fishy smell; (2) the color is dark and dark yellow; (3) the salt content is as high as more than 10%; (4) due to the molecular weight distribution is not Uniform, affects digestibility and often has poor organoleptic properties

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Shark cartilage collagen powder is first prepared with distilled water and is 20% aqueous solution by weight and is placed in the extraction tank, after the solution is clarified, n-hexane is added according to the weight percentage of 50% of the solution, stirred for 1 hour, and allowed to stand Extract for 6-8 hours; pass the lower layer solution through Sephadex G-10 gel chromatography column, elute with distilled water and collect the eluate; The weight percentage ratio of mixed enzymes is added, and the weight ratio of the mixed enzymes is collagenase: Bacillus subtilis 1.398 neutral protease: Aspergillus terreus 3.942 protease = 1:2:1; then enzyme digestion under constant stirring for 2-4 hours, After enzymatic digestion, add powdered activated carbon according to the weight percentage of 6% cartilage collagen powder, stir and decolorize at 45-51°C for 0.5 hours, filter, vacuum concentrate, and then spray dry to obtain colorless, odorless, fat-free ,...

Embodiment 2

[0014] Embodiment 2: Collagen powder obtained after extracting chondroitin sulfate from pig nasal bone is used as raw material, the steps and method are the same as in Example 1, and the organic solvent is selected as petroleum ether to obtain a product of the same quality.

Embodiment 3

[0015] Embodiment 3: Collagen powder obtained after extracting chondroitin sulfate from bovine cartilage is used as raw material, adopts the process and conditions of embodiment 1, and selects the organic solvent as diethyl ether to obtain a product of the same quality.

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PUM

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Abstract

The invention relates to a process method for preparing a collagen polypeptide through a high-value deep processing technology by using collagen powder obtained through extraction of chondroitin sulfate from animal cartilage as a raw material. The process method comprises: preparing an aqueous solution from animal cartilage collagen powder, clarifying, stirring in an organic solvent, carrying outstanding extraction to remove lipid components and odor, eluting the salt components of the lower layer solution by using a gel chromatographic column filtration method, collecting the eluent, heatingthe eluent to a temperature of 40-55 DEG C, carrying out directed digestion on the collagen powder under a stirring condition by selecting mixing enzyme, carrying out stirring decolorization with active carbon under a temperature control condition, filtering, concentrating, and drying to obtain hydrolyzed collagen polypeptide. According to the present invention, the method has characteristics ofsimple process, easily available raw materials, high production efficiency, excellent product quality, pure product odor, low cost and good chemical performance of the product, and is suitable for large industrial production.

Description

Technical field: [0001] The invention belongs to the technical field of food chemical industry, and relates to a process for preparing collagen polypeptides through high-value deep processing technology using collagen powder obtained after extracting chondroitin sulfate from animal cartilage as a raw material, in particular to a method for extracting animal collagen polypeptides. Background technique: [0002] At present, the technology of deepening the preparation of high-quality collagen peptides with collagen powder as raw material is arousing widespread interest. Collagen is the most abundant and widely distributed protein with immunological properties in animals. It widely exists in animal skin, bones, teeth, tendons, ligaments and blood vessels, accounting for 30% of the total protein in mammals. It is widely used in the pharmaceutical industry, biomedical raw materials and food industry, imaging industry, etc. The earliest collagen products produced using leather scr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
CPCC12P21/06C07K14/78
Inventor 尤世元孙亮
Owner 刘明明
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