Method for rapidly detecting broiler cecal proteolytic bacteria

Abstract

The invention relates to a method for rapidly detecting broiler cecal proteolytic bacteria. The method comprises the following steps: step (1) of collecting a sample; step (2) of diluting the sample;step (3) of eluting cells; step (4) of collecting filtrate; step (5) of collecting precipitate; step (6) of calibrating broiler cecal proteolytic bacteria; step (7) of performing return identificationof the broiler cecal proteolytic bacteria; and step (8) of performing abundance determination and statistical analysis of the broiler cecal proteolytic bacteria. The method greatly reduces the laborintensity, the cost is low, the operation process is simple and convenient, experimental results are accurate, relevant information and data can be quickly provided for feeding workers, the process ofscreening a high quality feed formula is shortened, and information and data on the broiler cecal protein degradation bacteria can be quickly provided for the feeding workers, to determine the extentto which a particular feed formula affects the performance and health of broilers under special feeding conditions.

Description

technical field [0001] The invention relates to a method for rapidly detecting proteolytic bacteria, in particular to a method for rapidly detecting proteolytic bacteria in caecum of broilers. Background technique [0002] Intestinal health of broilers is one of the necessary conditions for broiler growth. The balance of gut flora plays an important role in maintaining a healthy gut. Disturbances in gut homeostasis can affect gut function and health, leading to physiological disturbances that compromise broiler health and lead to reduced growth performance. Studies on sterile broilers have shown that intestinal microbes can promote intestinal maturation, improve body immunity, inhibit pathogenic bacteria, promote broiler growth, degrade indigestible food, and produce volatile fatty acids and vitamins. important role in development and function. [0003] In recent years, as the broiler chicken feeding cycle has decreased year by year, the current broiler chicken can reach ...

AI Technical Summary

Problems solved by technology

[0011] So far, there is no effective, simple, and easy-to-operate method that can be used to identify and quantify broiler cecal proteolytic bacteria in situ, and the identification and quantification of broiler cecal proteolytic bacteria can be used indirectly to monitor The level of broiler cecal protein fermentation, in order to speculate the impact of broiler cecal protein fermentation on the health and performance of broiler chickens, especially for further optimizing the impact of different dietary formulas on the health and performance of broiler chickens has far-reaching significance

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