Bionic bone scaffold and preparation method thereof

A bionic bone and porous scaffold technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of low practicability, imperfection, and lack of rigid structure of the scaffold, so as to avoid the "stress shielding" effect and enhance the induction , to meet the effect of clinical application

Inactive Publication Date: 2018-06-29
LOGISTICS UNIV OF CAPF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Second, most of the research is only on the surface of the film or the surface of a very thin 3D structure to simulate ECM modification. Due to the lack of rigid structural factors, the practicability of such scaffolds is not high.
[0006] The third is that the pure chemical environment is not perfect in promoting tissue regeneration, and at the same time restores the mechanical environment of the defective tissue, and the physiological environment plays an important role in inducing tissue regeneration

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method based on ECM bionic bone support, comprising the steps of:

[0019] (1) Select polycaprolactone (PCL) with a viscosity-average molecular weight of 43,200-50,000 as raw material, and prepare a bone scaffold by melting 3D printing. The fiber diameter is 0.3 mm; the fiber spacing is 0.3 mm; The spacing between the layers is 0.3mm, 3D printed into a cube porous scaffold of 50×50×3mm, cut into a cylindrical scaffold with a diameter of 6mm and a thickness of 3mm with a mold, and it is ready for use after disinfection.

[0020] (2) Prepare α-MEM medium containing 10% FBS by volume and 1% double antibody by volume, and prepare osteoblast cell line at 12000 / cm 2 Appropriate density inoculation in the culture flask, in the volume concentration of 5% CO 2 , cultured in a 37°C cell culture incubator. When the cells reached 80% confluency, trypsinization was performed with 10 7 / cm 3 Density seeded on bone scaffolds. After 4 hours, the culture medium was ad...

Embodiment 2

[0023] (1) Polycaprolactone (PCL) with a viscosity-average molecular weight of 43,200-50,000 was selected as the raw material, and the bone scaffold was prepared by melting 3D printing. The fiber diameter was 0.1 mm; the fiber spacing was 0.1 mm; The space between the layers is 0.1mm, 3D printed into a cube porous scaffold of 50×50×3mm, cut into a cylindrical scaffold with a diameter of 6mm and a thickness of 3mm with a mold, and it is ready for use after disinfection.

[0024] (2) Prepare α-MEM medium containing 10% FBS by volume and 1% double antibody by volume, and prepare osteoblast cell line at 12000 / cm 2 Appropriate density inoculation in the culture flask, in the volume concentration of 5% CO 2 , cultured in a 37°C cell culture incubator. When the cells reached 80% confluency, trypsinization was performed with 10 4 / cm 3 Density seeded on bone scaffolds. After 4 hours, the culture medium was added and cultured in a cell culture incubator.

[0025] (3) Apply a mecha...

Embodiment 3

[0027] (1) Select polycaprolactone (PCL) with a viscosity-average molecular weight of 43,200-50,000 as the raw material, and prepare the bone scaffold by melting method 3D printing. The fiber diameter is 0.3 mm; the fiber spacing is 0.7 mm; The distance between the layers is 0.3mm, 3D printed into a cube porous scaffold of 50×50×3mm, cut into a cylindrical scaffold with a diameter of 6mm and a thickness of 3mm with a mold, and it is ready for use after disinfection.

[0028] (2) Prepare α-MEM medium containing 10% FBS by volume and 1% double antibody by volume, and prepare osteoblast cell line at 12000 / cm 2 Appropriate density inoculation in the culture flask, in the volume concentration of 5% CO 2 , cultured in a 37°C cell culture incubator. When the cells reached 80% confluency, trypsinization was performed with 10 5 / cm 3 Density seeded on bone scaffolds. After 4 hours, the culture medium was added and cultured in a cell culture incubator.

[0029] (3) Apply a mechanic...

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Abstract

The invention discloses a bionic bone scaffold and a preparation method thereof. The bionic bone scaffold is a porous support by three-dimensionally printing polycaprolactone into a different structure through a melting method, an osteoblast extracellular matrix is adhered to a support fiber as a coating, an ECM coating acts osteoblasts by periodic pressure stress under different physiological stresses, and the bionic bone scaffold is obtained after cell removal treatment. The bionic bone scaffold provides structural characteristics and mechanical characteristics meeting cancellous bone, the ECM coating provides bioactive ingredients to induce osteogenesis, the quality and quantity of active ingredients are strengthened through mechanical stimulation, PCL has biodegradability, a synergistic effect can be played effectively through structural and mechanical properties and biological induced activity there-dimensional simulation, so that the bone scaffold has practicability and functionality and meets clinic application.

Description

technical field [0001] The invention relates to a bionic bone support and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] The repair of large bone defects and the functional reconstruction of bones with specific shapes are major problems in orthopedic clinical treatment. Simply using autologous bone grafting or allogeneic bone grafting, or using various artificial bone substitute materials such as metals, ceramics, and polymers in biological It is difficult to achieve satisfactory results in terms of physical and mechanical functions. Constructing tissue-engineered bone with vital activity and inducible tissue regeneration has become a frontier topic in the repair of bone defects. Bioactivity-inducing materials have become a fundamental and important factor in the field of tissue engineering because they adopt the concept of bionics and construct a good material environment conducive to cell regeneration by simulatin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/18A61L27/28A61L27/50A61L27/36
CPCA61L27/56A61L27/18A61L27/28A61L27/3633A61L27/365A61L27/3687A61L27/50A61L2430/02C08L67/04
Inventor 刘璐
Owner LOGISTICS UNIV OF CAPF
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