Method of treating petroleum-polluted clay foundation by using multiple microflora
A microbial composite bacteria, oil pollution technology, applied in the direction of microorganism-based methods, restoration of contaminated soil, biochemical equipment and methods, etc., can solve the problems of microorganisms that cannot migrate freely, achieve low noise, reduce soil toxicity, and energy The effect of low consumption
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Embodiment 1
[0023] In this example, a method for treating petroleum-contaminated clay foundations by a microbial complex is used to treat petroleum-contaminated clay foundations in a certain area. The plane size of the site is: 160m long, 120m wide, and the thickness of the soil layer to be treated is 3.1m to 3.5m , the method includes the following steps:
[0024] a. Extract soil pore fluid from the site to be treated and put it into liquid medium, and inoculate engineering bacteria in it to obtain a domesticated composite flora, which consists of Bacillus pasteurii, Bacillus subtilis (Bacillus subtilis) and Bacillus pumilus (Bacillus pumilus), the biomass ratio of the three is 2:2:1, 1L liquid medium contains 5.0g soybean peptone, 15.0g tryptone, 5.0g chloride Sodium chloride and 10g of urea, the pH of the medium is controlled at 7.3, the domesticated flora together with the liquid medium and sterile soybean protein powder are mixed in a mass-volume ratio of 1:1, and left standing at 30...
Embodiment 2
[0031] In this example, a method for treating petroleum-contaminated clay foundations with a microbial complex is used to treat petroleum-contaminated silty clay foundations in a certain area. The plane size of the site is: 192m long, 156m wide, and the thickness of the soil layer to be treated is 4.2m~ 4.6m, the method includes the following steps:
[0032] a. Extract soil pore fluid from the site to be treated and put it into liquid medium, and inoculate engineering bacteria in it to obtain a domesticated composite flora, which consists of Bacillus pasteurii, Bacillus subtilis (Bacillus subtilis) and Bacillus pumilus (Bacillus pumilus), the biomass ratio of the three is 2:2:1, 1L liquid medium contains 5.0g soybean peptone, 15.0g tryptone, 5.0g chloride Sodium chloride and 10g of urea, the pH of the medium is controlled at 7.3, the domesticated flora together with the liquid medium and sterile soybean protein powder are mixed in a mass-volume ratio of 1:1, and left standing ...
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