Method of treating petroleum-polluted clay foundation by using multiple microflora

A microbial composite bacteria, oil pollution technology, applied in the direction of microorganism-based methods, restoration of contaminated soil, biochemical equipment and methods, etc., can solve the problems of microorganisms that cannot migrate freely, achieve low noise, reduce soil toxicity, and energy The effect of low consumption

Active Publication Date: 2018-06-29
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Technical problem: Due to the interaction between the electric double layer characteristics of clay mineral particles and microorganisms, as well as the compatibility of soil particle size, pore throat size and microbial individual size, microorganisms cannot freely migrate between the pores of cl

Method used

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  • Method of treating petroleum-polluted clay foundation by using multiple microflora
  • Method of treating petroleum-polluted clay foundation by using multiple microflora

Examples

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Embodiment 1

[0023] In this example, a method for treating petroleum-contaminated clay foundations by a microbial complex is used to treat petroleum-contaminated clay foundations in a certain area. The plane size of the site is: 160m long, 120m wide, and the thickness of the soil layer to be treated is 3.1m to 3.5m , the method includes the following steps:

[0024] a. Extract soil pore fluid from the site to be treated and put it into liquid medium, and inoculate engineering bacteria in it to obtain a domesticated composite flora, which consists of Bacillus pasteurii, Bacillus subtilis (Bacillus subtilis) and Bacillus pumilus (Bacillus pumilus), the biomass ratio of the three is 2:2:1, 1L liquid medium contains 5.0g soybean peptone, 15.0g tryptone, 5.0g chloride Sodium chloride and 10g of urea, the pH of the medium is controlled at 7.3, the domesticated flora together with the liquid medium and sterile soybean protein powder are mixed in a mass-volume ratio of 1:1, and left standing at 30...

Embodiment 2

[0031] In this example, a method for treating petroleum-contaminated clay foundations with a microbial complex is used to treat petroleum-contaminated silty clay foundations in a certain area. The plane size of the site is: 192m long, 156m wide, and the thickness of the soil layer to be treated is 4.2m~ 4.6m, the method includes the following steps:

[0032] a. Extract soil pore fluid from the site to be treated and put it into liquid medium, and inoculate engineering bacteria in it to obtain a domesticated composite flora, which consists of Bacillus pasteurii, Bacillus subtilis (Bacillus subtilis) and Bacillus pumilus (Bacillus pumilus), the biomass ratio of the three is 2:2:1, 1L liquid medium contains 5.0g soybean peptone, 15.0g tryptone, 5.0g chloride Sodium chloride and 10g of urea, the pH of the medium is controlled at 7.3, the domesticated flora together with the liquid medium and sterile soybean protein powder are mixed in a mass-volume ratio of 1:1, and left standing ...

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Abstract

A method of treating petroleum-polluted clay foundation by using multiple microflora is capable of achieving integration of soil recovery and soil reinforcing construction. The method includes the steps of: (a) preparing a microbial agent (1); (b) uniformly stirring the microbial agent (1) with soil (3) in to-be-processed stratum with a deep stirring machine (2) according to the mass ratio of 1:100, and then leveling the field; (c) arranging injection pipes (4) in a row in the field according to hole distance of 0.8 m; (d) injecting a nutrient solution (5) into the to-be-processed stratum through the injection pipes (4) every three days; (e) arranging detection holes in the field according to density of one hole per 200 m<2>, and withdrawing soil pore liquid from the detection holes everysix days and performing oil content measurement, when the oil content of the soil reduces by more than 80%, alternately injecting the nutrient solution (5) and a cementing solution (6) into the to-be-processed stratum through the injection pipes (4); and (f) when preset injection times is reached, detecting foundation strength and heavy metal concentration of the soil, if the indices satisfy requirements, removing the injection pipes (4), or if not, repeating the step (e) until the requirements are satisfied.

Description

technical field [0001] The invention relates to a method for treating petroleum-contaminated clay foundations with microbial compound flora, and belongs to the technical field of foundation treatment. Background technique [0002] The leakage of crude oil in the process of mining, production, and transportation has caused increasing concern about soil pollution, and its pollutants are mainly petroleum hydrocarbons and polycyclic aromatic hydrocarbons. Petroleum hydrocarbons are difficult to degrade in the environment. When they enter the soil, they will change the nutrient composition of the soil, affect the permeability of the soil, and cause crop death. The vast majority of aromatic hydrocarbons and their derivatives are carcinogenic and mutagenic, which seriously endanger human health. In addition, petroleum-contaminated soil also contains a variety of harmful heavy metals, which also pollute soil and groundwater. With the acceleration of urbanization in my country, the...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/07C12R1/125
CPCB09C1/10C12N1/20
Inventor 黄容聘邵光辉刘鹏
Owner NANJING FORESTRY UNIV
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