A kind of recombinant Artemisia annua class 1 allergen protein and its application

A technology of allergen and Artemisia annua, applied in the direction of allergen antigen composition, application, recombinant DNA technology, etc., can solve the problems of difficult expression, diagnostic specificity, unsatisfactory curative effect, pollution, etc.
CN108265065BActive Publication Date: 2021-04-27ZONHON BIOPHARMA INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZONHON BIOPHARMA INST
Publication Date
2021-04-27

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Abstract

The invention relates to Art a1, the main allergenic protein of Artemisia annua expressed in mammalian cells, encoding gene, expression method, purification process, detection method and application of recombinant protein. The Artemisia annua allergen protein itself is a plant protein, and it is difficult to express it in the existing prokaryotic or eukaryotic expression systems, and it has not been disclosed in relevant literature; however, traditional extraction methods often have complex components, difficult quality control, Vulnerable to contamination by exogenous toxic substances, pathogenic microorganisms, etc. For this reason, the present invention provides the coding gene of the recombinant Art a1 shown in SEQ ID NO: 1, and expression, purification, recombinant protein detection method and application specially designed for this purpose, compared with the prior art, the present invention Recombinant Art a1 has a relatively high expression level and activity, and also establishes a foundation for cell line development, process optimization, preclinical and clinical research, and industrial production in the drug development process.
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Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a recombinant Artemisia annua class 1 allergen protein, its coding gene and an expression and purification method. Background technique

[0002] In recent years, the incidence of allergic hay fever has been increasing year by year, and the incidence in Europe has risen from 1% in the early 20th century to 20%, and it is expected to reach 35% in the next 20 years. There are more than 10 million hay fever patients in my country, the incidence rate of urban residents is about 0.9%, and it can reach 5% in endemic areas. With more and more varieties of trees and flowers in urban areas, the number of hay fever patients is increasing.

[0003] Pollen is the male reproductive cell of higher plants. It is spread in the air under the action of wind or insects. Some people will have allergic reactions after inhaling pollen during breathing. The dispersal of pollen ha...

Claims

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