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Preparation technology of lysine fermentation medium

A lysine fermentation and culture medium technology, which is applied in the field of amino acid fermentation, can solve the problems of low lysine yield and high cost of fermentation medium, and achieve the effect of easy enzymolysis, low cost and reduced enterprise cost

Active Publication Date: 2020-11-10
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects of high cost of fermentation medium and low yield of lysine in the prior art, and provide a preparation process for lysine fermentation medium

Method used

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  • Preparation technology of lysine fermentation medium
  • Preparation technology of lysine fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A lysine fermentation medium, which comprises the following components:

[0028] Corn husk treatment 300g / L, corn steep liquor 10g / L, potassium dihydrogen phosphate 2g / L, dipotassium hydrogen phosphate 2g / L, ferrous sulfate heptahydrate 0.2g / L, magnesium sulfate heptahydrate 0.2g / L, acetic acid Calcium 200mg / L, Mannitol 50mg / L, Biotin 10mg / L, Vitamin B 1 2mg / L, the solvent is water;

[0029] The preparation process is as follows: adding various raw materials into water, adjusting the pH to 6.8, sterilizing at 121° C. for 20 minutes, and cooling to 32° C. to obtain the product.

[0030] The fermentation process using the above-mentioned fermentation medium includes the following steps: placing the fermentation medium in a fermenter, then inserting the strain seed liquid according to the inoculation amount of 12%, controlling the temperature at 32°C, the tank pressure at 0.05MPa, and the air volume at 600L / h, the rotation speed is 350rpm, the pH is controlled at 6.8, t...

Embodiment 2

[0036]A lysine fermentation medium, which comprises the following components:

[0037] Corn husk treatment 200g / L, corn steep liquor 20g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, ferrous sulfate heptahydrate 0.1g / L, magnesium sulfate heptahydrate 0.1g / L, acetic acid Calcium 100mg / L, Mannitol 100mg / L, Biotin 5mg / L, Vitamin B 1 1mg / L, the solvent is water;

[0038] The preparation process is as follows: adding various raw materials into water, adjusting the pH to 6.8, sterilizing at 121° C. for 20 minutes, and cooling to 32° C. to obtain the product.

[0039] The fermentation process using the above-mentioned fermentation medium includes the following steps: placing the fermentation medium in a fermenter, then inserting the strain seed liquid according to the inoculum amount of 10%, controlling the temperature at 32°C, the tank pressure at 0.05MPa, and the air volume at 600L / h, the rotation speed is 350rpm, the pH is controlled at 6.8, the d...

Embodiment 3

[0054] Using Corynebacterium glutamicum ATCC14997 as the fermentation strain, the amount of lysine produced by fermentation in Example 1 and Comparative Examples 1-3 was studied. The fermentation process was referred to in Example 1. The specific acid production by fermentation is shown in Table 1:

[0055] Table 1

[0056] group Example 1 Comparative example 1 Comparative example 2 Comparative example 3 Lysine production g / L 173.5 147.8 162.9 151.7

[0057] As shown in Table 1, the amount of acid production in Example 1 is the highest, which is 17.4% higher than that of Comparative Example 1, 6.5% higher than that of Comparative Example 2, and 14.3% higher than that of Comparative Example 3.

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Abstract

The invention belongs to the technical field of lysine fermentation, and discloses a preparation process of a lysine fermentation culture medium. According to the process, corn bran treatment substances, corn steep liquor, monopotassium phosphate, dipotassium phosphate, iron vitriol, calcium acetate, mannitol, biotin and vitamin are sequentially added into water; uniform stirring is performed; the pH is regulated to 6.8; heat insulation is performed at 121 DEG C for 20min for sterilization; cooling is performed; the lysine fermentation culture medium is obtained. The preparation process is simple; the implementation is easy; the corn bran is used as a major raw material; the prepared culture medium is low in cost.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation, and in particular relates to a preparation process of a lysine fermentation medium. Background technique [0002] Lysine is an amino acid needed by the human body, an indispensable nutrient. Protein is the main component of human cells. After entering the human body, protein in food is first decomposed into amino acids after digestion, and then the human body uses these amino acids to synthesize new human proteins, such as immune antibodies, digestive enzymes, plasma proteins, growth hormones, etc. It is a synthetic human protein. Among the various amino acids that synthesize proteins, L-lysine is the most important one. Without lysine, other amino acids are limited or cannot be used. Scientists call it the first essential amino acid for the human body. Scientists have also discovered that L-lysine is the most important and essential component of somatostatin, an important subst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/15
CPCC12P13/08
Inventor 董力青王铮王峰唐永强汲广习李航聂庆胜
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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