Phosphorus-accumulating bacteria immobilized small balls and application thereof

A technique of immobilizing small balls and phosphorus-accumulating bacteria, which is applied in the fields of immobilized on/in organic carriers, chemical instruments and methods, water pollutants, etc. Large loss and other problems, to achieve the effect of good mechanical strength, large adsorption capacity and high viscosity

Active Publication Date: 2018-08-03
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of boric acid cross-linking agents in the preparation of polyvinyl alcohol gels is toxic to microbial cells, and the polyvinyl alcohol carrier is prone to adhesion during use, resulting in embedded particles that are easily broken, large mass transfer resistance, and gas production. Defects such as floating and large loss of activity
The preparation of sodium alginate immobilized beads requires calcium ion cross-linking to form a gel, which can effectively embed microbial cells and has high microbial activity, but the stability of calcified sodium alginate gel is poor, and it is easy to react with multiple ions or When calcium ions diffuse and potassium and sodium ions gather in large quantities, it becomes soft, and the phenomenon of "leakage of enzyme" appears

Method used

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  • Phosphorus-accumulating bacteria immobilized small balls and application thereof
  • Phosphorus-accumulating bacteria immobilized small balls and application thereof
  • Phosphorus-accumulating bacteria immobilized small balls and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of immobilized pellet of phosphorus-accumulating bacteria is prepared by the following steps:

[0034] 1) Preparation of bacterial suspension: Inoculate phosphorus accumulating bacteria into enrichment medium, culture on a shaking table, obtain bacterial cells in logarithmic growth phase, centrifuge, discard supernatant, and inoculate bacterial cells with 0.9% normal saline Wash, centrifuge repeatedly 4-5 times, add the same volume of normal saline as the bacterial solution to dilute, and obtain the bacterial suspension;

[0035] The number of viable bacteria in the bacterial suspension prepared by the present invention is 1×10 6 -1×10 8 CFU / mL.

[0036] 2) Activated carbon adsorption: the bacterial suspension and activated carbon were mixed and adsorbed at room temperature for 30 minutes to obtain the activated carbon adsorbed bacterial suspension mixture;

[0037] 3) Preparation of γ-polyglutamic acid / oligochitosan hydrogel: Add γ-polyglutamic acid, hyaluron...

Embodiment 2

[0051] A kind of immobilized pellet of phosphorus-accumulating bacteria is prepared by the following steps:

[0052] 1) Preparation of bacterial suspension: Inoculate phosphorus accumulating bacteria into enrichment medium, culture on a shaking table, obtain bacterial cells in logarithmic growth phase, centrifuge, discard supernatant, and inoculate bacterial cells with 0.9% normal saline Wash, centrifuge repeatedly 4-5 times, add physiological saline equal to the volume of the bacterial solution to dilute to obtain a bacterial suspension;

[0053] The number of viable bacteria in the bacterial suspension prepared by the present invention is 1×10 6 -1×10 8 CFU / mL.

[0054] 2) Activated carbon adsorption: Mix the bacterial suspension with activated carbon at room temperature for 30 minutes to obtain the activated carbon-adsorbed bacterial suspension mixture;

[0055] 3) Preparation of γ-polyglutamic acid / oligochitosan hydrogel: add γ-polyglutamic acid, hyaluronic acid and oli...

Embodiment 3

[0069] A kind of immobilized pellet of phosphorus-accumulating bacteria is prepared by the following steps:

[0070] 1) Preparation of bacterial suspension: Inoculate phosphorus accumulating bacteria into enrichment medium, culture on a shaking table, obtain bacterial cells in logarithmic growth phase, centrifuge, discard supernatant, and inoculate bacterial cells with 0.9% normal saline Wash, centrifuge repeatedly 4-5 times, add physiological saline equal to the volume of the bacterial solution to dilute to obtain a bacterial suspension;

[0071] The number of viable bacteria in the bacterial suspension prepared by the present invention is 1×10 6 -1×10 8 CFU / mL.

[0072] 2) Activated carbon adsorption: Mix the bacterial suspension with activated carbon at room temperature for 30 minutes to obtain the activated carbon-adsorbed bacterial suspension mixture;

[0073] 3) Preparation of γ-polyglutamic acid / oligochitosan hydrogel: Add γ-polyglutamic acid, hyaluronic acid and oli...

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Abstract

The invention discloses phosphorus-accumulating bacteria immobilized small balls prepared by the following steps: preparation of a bacterial suspension; adsorption with activated carbon; preparation of a gamma-polyglutamic acid / chitosan oligosaccharide hydrogel; preparation of a composite embedded carrier aqueous solution; and preparation of the phosphorus-accumulating bacteria immobilized small balls. The invention also discloses an application of the phosphorus-accumulating bacteria immobilized small balls in treatment of phosphorus-rich waste water. The prepared phosphorus-accumulating bacteria immobilized small balls have the advantages of high activity, large adsorption capacity and good mechanical strength. With activated carbon as an adsorbent, the biosorption capacity of the phosphorus-accumulating bacteria immobilized small balls is significantly increased, the skeleton supporting role can also be played, and the mechanical strength of the phosphorus-accumulating bacteria immobilized small balls is enhanced; the gamma-polyglutamic acid / chitosan oligosaccharide hydrogel has the advantages of good biocompatibility, high viscosity, no toxicity, and good environmental friendliness, and significantly promotes the activity and adsorption capacity of the phosphorus-accumulating bacteria immobilized small balls, can also improve the mechanical strength and water content of thesmall balls, and enlarges the pore size of scaffolds in the phosphorus-accumulating bacteria immobilized small balls.

Description

technical field [0001] The invention belongs to the technical field of microorganism immobilization, and in particular relates to an immobilized pellet of phosphorus-accumulating bacteria and an application thereof. Background technique [0002] In recent years, under the double influence of natural factors and human factors, the phenomenon of eutrophication in my country's surface water has become increasingly serious. Phosphorus is the decisive factor in the problem of eutrophication in water bodies. Excessive phosphorus is the main cause of polluted and odorous water bodies, eutrophication of lakes and red tides in bays. The key to chemical control research. Phosphorus accumulating bacteria are a kind of special facultative bacteria that widely exist in eutrophic lake sediments. They release phosphorus anaerobically and absorb excessive phosphorus under aerobic conditions. They convert dissolved phosphorus in water into insoluble polyphosphate. Stored in the cells, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/10C02F3/34
CPCC02F3/34C02F2101/105C12N11/08C12N11/10
Inventor 杨革安笑迟车程川刘金锋巩志金梁鑫鑫
Owner QUFU NORMAL UNIV
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