Preparation method of electrochemical alanine biosensor based on boron-doped diamond microelectrode and application thereof

A biosensor, boron-doped technology, applied in the field of electrochemical biosensors, can solve the problems of poor selectivity and sensitivity, difficult surface reconstruction, difficult mass production, etc., achieve good sensitivity and detection range, avoid low stability, The effect of reduced steps

Active Publication Date: 2018-08-03
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at the same time, boron-doped diamond electrodes also have disadvantages such as low electrocatalytic activity, difficult surface reconstruction, poor selectivity and sensitivity, and difficulty in large-scale production, which limits its application.

Method used

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  • Preparation method of electrochemical alanine biosensor based on boron-doped diamond microelectrode and application thereof
  • Preparation method of electrochemical alanine biosensor based on boron-doped diamond microelectrode and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for manufacturing an electrochemical alanine biomicrosensor based on a boron-doped diamond microelectrode, the specific steps are as follows:

[0030] (1) Put the P-type single crystal silicon wafer as the substrate into an acetone solution mixed with diamond micropowder for ultrasonic treatment for 5 minutes, and put it into the acetone solution for ultrasonic cleaning after uniform and dense micro scratches are formed on the surface of the substrate 3min. This process is called pretreatment of the substrate.

[0031] (2) Put the processed P-type single crystal silicon wafer into the hot wire chemical vapor deposition system. The temperature of the hot wire is controlled at 2000-3000°C, the substrate temperature is controlled at 800°C, and the distance between the filament and the substrate is maintained at 4mm. in CH 4 and H 2 nucleation in atmosphere, CH 4 The concentration is 7% (volume fraction), the air pressure is kept at 2kPa, and the nucleation tim...

Embodiment 2

[0038] A method for manufacturing an electrochemical alanine biomicrosensor based on a boron-doped diamond microelectrode, the specific steps are as follows:

[0039] (1) Put the N-type single crystal silicon wafer as the substrate into an acetone solution mixed with diamond powder for ultrasonic treatment for 10 minutes, and after uniform and dense micro scratches are formed on the surface of the substrate, put it into the acetone solution for ultrasonic cleaning for 5 minutes .

[0040] (2) Put the processed N-type single crystal silicon wafer into a microwave plasma chemical vapor deposition system, and control the substrate temperature at 900°C. in CH 4 and H2 nucleation in atmosphere, CH 4 The concentration is 6%, the air pressure is kept at 1kPa, and the nucleation time is controlled at 8min. During the growth process, methane was used as the carbon source and diborane was used as the boron source (B / C=1000ppm), the growth pressure was maintained at 2kPa, and the depo...

Embodiment 3

[0047] A method for manufacturing an electrochemical alanine biomicrosensor based on a boron-doped diamond microelectrode, the specific steps are as follows:

[0048] (1) Put the quartz wafer used as the substrate into an acetone solution mixed with diamond micropowder for ultrasonic treatment for 10 minutes, and after uniform and dense micro-scratches are formed on the surface of the substrate, put it into the acetone solution for ultrasonic cleaning for 4 minutes.

[0049] (2) Put the processed quartz sheet into the DC plasma jet chemical vapor deposition system, the gas ratio is H 2 :Ar:CH 4 =2000:1500:50, the unit is sccm (mL / min). Boron oxide was put into a vacuum chamber for volatilization as a boron source (B / C=4300ppm), and the air pressure was kept at 5kPa. The substrate temperature was 900°C, and the growth time was 3h. After the reaction, a quartz plate with a layer of boron-doped diamond film deposited on the surface was obtained, wherein the thickness of the bo...

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Abstract

The invention relates to a preparation method of an electrochemical alanine biosensor based on a boron-doped diamond microelectrode and an application of the preparation method of the biosensor in alanine detection. The method comprises the following steps: employing a vapour deposition method to prepare a boron-doped diamond film at a substrate; employing a physical vapour deposition method for depositing a layer of film coating of metal and/or metal oxide on the surface of the boron-doped diamond film; employing a lithography technology to prepare an electrode pattern on the film coating, and after pickling, completing the preparation of a mask film; employing an etching technology for etching the surface of the boron-doped diamond to prepare a boron-doped diamond microelectrode; and performing nickel deposition modification at the boron-doped diamond microelectrode. The electrochemical alanine biosensor can greatly improve the electrode performance, increases the electrode sensitivity, and has considerable application prospect while being used for detecting the alanine concentration.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical biosensor based on a boron-doped diamond microelectrode, which can be used to detect the concentration of alanine. Background technique [0002] Alanine is an important nutrient in the human body. According to the current clinical diagnosis, if there is a lack of alanine in the blood, it will lead to ketogenic hypoglycemia, Sheehan's syndrome and hypopituitarism. Therefore, accurate detection of alanine concentration is of great significance in clinical diagnosis and biopharmaceuticals. [0003] Some current alanine detection methods mainly include: flow injection method, chromatographic analysis method, atomic absorption method, and colorimetric method. However, these methods have problems such as high cost, long time, and unsuitability for routine detection. The new electrochemical sensor analysis method not only solves the above problems, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 许青波王传新王涛
Owner WUHAN INSTITUTE OF TECHNOLOGY
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