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Extraction and purification method of dendrobium officinale leaf total flavone

A technology of dendrobium candidum and purification method, which is applied in food extraction, medical formula, and the function of food ingredients, etc., can solve the problems of less research on flavonoid carbon glycosides, and achieve clear effects, small doses, and good biological activity.

Inactive Publication Date: 2018-08-17
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The 8 flavonoid carbon glycosides that have been identified in the leaves of Dendrobium officinale are all flavonoid dicarbon glycosides with apigenin as the aglycone. relatively little research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] An extract from total flavonoids of Dendrobium officinale leaves is obtained through the following steps:

[0016] (1) According to the feeding ratio, weigh the dried leaves of Dendrobium officinale, and the ratio of material to liquid (that is, the ratio of the weight of Dendrobium candidum leaves to the volume of ethanol solvent, the same below) is 1:8, add 75% ethanol solvent, and extract under reflux at 80°C 2 times, the filtrate obtained twice was combined, concentrated under reduced pressure, and ethanol was removed to obtain the ethanol extract of Dendrobium officinale leaves;

[0017] (2) Add petroleum ether solvent to the ethanol extract of Dendrobium officinale leaves at an extraction ratio (volume ratio of ethanol extract of Dendrobium officinale leaves to extractant, the same below) 1:0.5, extract in an extraction tank, and discard the petroleum ether phase, keep the water phase, repeat the operation 2 times, add n-butanol solution to the water phase at an e...

Embodiment 2

[0022] An extract from total flavonoids of Dendrobium officinale leaves is obtained through the following steps:

[0023] (1) According to the feeding ratio, weigh the dried leaves of Dendrobium candidum, add 80% ethanol solvent at a material-to-liquid ratio of 1:12, reflux extraction at 60°C for 3 times, combine the filtrate obtained twice, concentrate under reduced pressure, and remove ethanol to obtain Alcoholic extract of Dendrobium officinale leaves.

[0024] (2) Add petroleum ether solvent to the ethanol extract of Dendrobium officinale leaves at an extraction ratio of 1:1, extract in an extraction tank, discard the petroleum ether phase, and keep the water phase. The extraction ratio is 1:1, add n-butanol solution, extract in the extraction tank, discard the water phase, keep the n-butanol phase, and repeat the operation twice to obtain the n-butanol phase, concentrate under reduced pressure, and remove n-butanol The solvent and the extract are redissolved with water t...

Embodiment 3

[0029] An extract from total flavonoids of Dendrobium officinale leaves is obtained through the following steps:

[0030] (1) According to the feeding ratio, weigh the dried leaves of Dendrobium candidum, add 75% ethanol solvent at a material-to-liquid ratio of 1:10, reflux extraction at 80°C for 3 times, combine the filtrate obtained twice, concentrate under reduced pressure, and remove ethanol to obtain Alcoholic extract of Dendrobium officinale leaves.

[0031] (2) Add petroleum ether solvent to the ethanol extract of Dendrobium officinale leaves at an extraction ratio of 1:2, extract in an extraction tank, discard the petroleum ether phase, and keep the water phase. The extraction ratio is 1:2, add n-butanol solution, extract in the extraction tank, discard the water phase, keep the n-butanol phase, and repeat the operation 3 times to obtain the n-butanol phase, concentrate under reduced pressure, and remove n-butanol The solvent and the extract are redissolved with water...

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PUM

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Abstract

The invention discloses an extraction and purification method of dendrobium officinale leaf total flavone. The method includes following steps: mixing dendrobium officinale leaves with an alcohol water solution, performing backflow extraction, filtering, evaporating and concentrating filtrate, using petroleum ether and normal butanol to extract obtained concentrate, concentrating obtained extract,preparing a deionized water solution, using macroporous resin for adsorption and enrichment, using the alcohol water solution for elution, and concentrating eluate to obtain a dendrobium officinale leaf total flavone product. The total flavone after extraction and purification does not contain harmful impurities and is high bioactivity. The macroporous resin is renewable and reusable, alcohol obtained by evaporation and concentration can be reutilized, and little effluent pollution is caused in the process.

Description

technical field [0001] The invention belongs to the technical fields of natural product separation, health food and medicine, and in particular relates to a method for extracting and purifying total flavonoids from dendrobium officinale leaves. Background technique [0002] Modern studies have shown that total flavonoids have various biological activities such as lowering blood pressure, lowering blood fat, immune regulation, and scavenging free radicals, especially for the significant inhibition of systolic and diastolic blood pressure. In addition, total flavonoids have low toxicity or even no toxicity to normal cells of the human body, so total flavonoids have broad application prospects in human health care, nutrition, and disease prevention and treatment. [0003] Dendrobium officinale Kimuraet Migo is a rare Chinese herbal medicine, the dried stem of the orchid plant Dendrobium officinale Kimuraet Migo, and it is the first of the nine immortal grasses. It has traditio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A23L33/105A61K127/00
CPCA23L33/105A23V2002/00A61K36/8984A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23V2200/30A23V2250/2116A23V2300/14
Inventor 陈素红吕圭源娄晓晶陆婷婷占靓卉李波王昱植贺行理商
Owner ZHEJIANG UNIV OF TECH
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