Method for separating, identifying and preliminary screening of probiotic yak-derived lactic acid bacteria

Abstract

The invention belongs to the field of biotechnology, and discloses a method for separating, identifying and preliminary screening of probiotic yak-derived lactic acid bacteria. The separation steps comprise: taking about 1 g of the middle portion of a manure sample, adding into a sterilized and refrigerated peptone buffer solution, sucking 1 mL of the manure supernatant under a sterile condition,inoculating into a MRS liquid culture medium, respectively carrying out aerobic culture and anaerobic culture for 24 h at a temperature of 37 DEG C, taking 200 [mu]l of the bacterial suspension, coating a MRS plate with the bacterial suspension, respectively carrying out aerobic culture and anaerobic culture for 24 h, selecting single suspected colony, inoculating into a corresponding plate culture medium, carrying out streak culture, respectively carrying out aerobic culture and anaerobic culture for 24 h at a temperature of 37 DEG C, re-selecting single colony, inoculating into a corresponding plate culture medium, respectively carrying out aerobic culture and anaerobic culture at a temperature of 37 DEG C, purifying twice, selecting single colony, inoculating into a corresponding slopeculture medium, respectively carrying out aerobic culture and anaerobic culture for 24 h at a temperature of 37 DEG C, and storing in a 4 DEG C a refrigerator so as to be spare. According to the present invention, the probiotic yak-derived lactic acid bacteria can protect the intestinal mucosa so as to reduce intestinal epithelium inflammation and diarrhea.

Description

technical field [0001] The invention specifically relates to a method for the isolation, identification and preliminary screening of probiotic yak-derived lactic acid bacteria, which belongs to the field of biotechnology. Background technique [0002] Calf diarrhea disease has always been an important disease affecting the yak breeding industry in Tibet. Its causes are complex and mostly occur in the alternate seasons of winter and spring. It occurs in all counties of Tibet. According to the survey, in Bangor County, among the calves that died in spring Diarrhea killed up to 70%. Due to the irrational use of antibiotics in the treatment of the disease, there are serious multi-drug resistances in many pathogenic bacteria in yaks. Therefore, there is an urgent need to find effective and low-risk methods for the treatment and prevention of diarrheal disease in calves. [0003] Probiotics are live microorganisms that are beneficial to the health of the host when ingested in su...

AI Technical Summary

Problems solved by technology

Due to the irrational use of antibiotics in the treatment of the disease, there are serious multi-drug resistances in various pathogenic bacteria in yaks

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