Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Distant hybridization method of common head cabbage and cabbage type rape

A technology of Brassica napus and distant hybridization, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of inability to obtain hybrid plants, abortion of immature embryos, etc.

Inactive Publication Date: 2018-08-28
NORTHWEST A & F UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the object of the present invention is to provide a method for distant hybridization between head cabbage and Brassica napus, which overcomes the problem that when cabbage and Brassica napus cultivars are distantly hybridized, abortion of immature embryos is likely to occur and hybrid plants cannot be obtained , transfer the excellent restorer gene of rapeseed into the cytoplasmic male sterile cultivar of cabbage to restore the fertility of cabbage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Distant hybridization method of common head cabbage and cabbage type rape
  • Distant hybridization method of common head cabbage and cabbage type rape
  • Distant hybridization method of common head cabbage and cabbage type rape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The female parent was selected from the cabbage Ogura cytoplasmic male sterile line cultivar Qingan 58 (2n=2x=18), and the male parent was selected from the rapeseed excellent restorer cultivar (2n=4x=38); selected disease-free and plump parents The seeds are prepared for planting and routinely managed;

[0052] Remove the flowers that have opened on the female parent's inflorescence 3 days before the hybridization at the initial flowering stage, tag the unopened flowers and bag them for isolation, take the anthers on the flowering day of the bagged male parent, and peel off the 4mm flower buds on the female parent plant for pollination ;

[0053] Cut the ovary 16 days after pollination, soak it in 70% ethanol for 30s, and then wash it with 0.1% HgCl 2 Disinfect for 15 minutes, then rinse with sterile water 4 times, 5 minutes each time;

[0054] The sterilized ovaries were peeled off the immature embryos on sterile filter paper under aseptic conditions and inoculated ...

Embodiment 2

[0059] The female parent was selected from the cabbage Ogura cytoplasmic male sterile line cultivar Qingan 68 (2n=2x=18), and the male parent was selected from the excellent restorer line of rapeseed cultivar RFO-46 (2n=4x=38); selected disease-free Full parental seeds ready for planting, routine management;

[0060] Remove the bloomed flowers on the female parent's inflorescence 2 days before hybridization at the initial flowering stage, tag the unopened flowers and bag them for isolation, take the anthers on the flowering day of the bagged male parent, and peel off the 4mm flower buds on the female parent plant for pollination ;

[0061]Cut the ovary 15-18 days after pollination, soak it in 70% ethanol for 30s, and then wash it with 0.1% HgCl 2 Disinfect for 15 minutes, then rinse with sterile water 4 times, 5 minutes each time;

[0062] The sterilized ovaries were peeled off the immature embryos on sterile filter paper under aseptic conditions and inoculated on the ovule ...

experiment example

[0067] The 55 hybrid plants that embodiment 1 and embodiment 2 obtain altogether carry out hybrid verification:

[0068] (1) Cytological identification: Sysmex CyFlow Cube8 flow cytometer was used to identify the ploidy of the hybrid offspring.

[0069] Prepare the solution: solution I is 4.2g citric acid, 0.5% Tween 20, dilute to 200mL, store at 4°C for later use; solution II is 28.65g sodium dihydrogen phosphate, PI20mg, dilute to 200mL.

[0070] Take 20mg of parent and hybrid F 1 The new leaves of the generation seedlings were placed in a plastic petri dish, and the leaves were chopped with a double-sided blade, and the cut samples were sucked up with a 1mL pipette gun and filtered into a 1.5mL Eppendorf tube through a 350-mesh nylon screen, and fresh I Suspend the sediment in 400 μL of the solution, add 800 μL of solution II to the sample when measuring the sample with a flow cytometer, and judge the hybrid F according to the abscissa position of the fluorescence intensit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a distant hybridization method of common head cabbage and cabbage type rape and belongs to the field of biotechnological breeding. The method comprises the following steps: (1)selecting cytoplasmic male sterile line cultivated species of common head cabbage as female parents, taking restorer line cultivated species of cabbage type rape as male parents, carrying out artificial pollination and then isolating the female parents and the male parents; (2) cutting ovaries which are pollinated before 15-18 days, and disinfecting the ovaries; (3) peeling ovules of the disinfected ovaries and carrying out induction culture until the ovules are germinated; (4) carrying out differentiation culture of the germinated ovules to obtain an F1 generation of seedlings; and (5) separating lateral buds of the F1 generation of seedlings and inoculating a rooting culture medium with the lateral buds to obtain complete hybridized seedlings. According to the distant hybridization method, an embryo rescue technique is used, the problem that the hybrid plants cannot be obtained because of high possibility of abortion of young embryos when the cultivated species of common head cabbageand cabbage type rape are distantly hybridized can be overcome; excellent restoration genes of the rape are transferred into cytoplasmic male sterile line cultivated species of common head cabbage, so that the fertility of the cabbage can be restored; the technical basis is provided for innovation and application of genetic resources of the cabbage.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, in particular to a method for distant hybridization of head cabbage and Brassica napus. Background technique [0002] Heading cabbage (Brassica oleracea L.var.capitata L., 2n=18), referred to as cabbage, belongs to Brassicaceae Brassica cabbage species. It is an important vegetable crop in China and is cultivated all over the country. The annual cultivation area reaches 9 ×10 5 hm 2 about. The heterosis of cabbage is more obvious. The materials of Ogura cytoplasmic male sterile line show maternal inheritance, pollen abortion and normal pistil, and are easy to restore fertility by the dominant nuclear restorer gene of the restorer material, but cannot carry out normal self-segregation and further innovation of germplasm resources . [0003] Both head cabbage and Brassica napus are important cultivated crops in the Brassicaceae. The rapeseed material is also a restorer line material. The ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008
Inventor 许忠民王杏利张恩慧姜娇李升娟郭佳马青山石汶汶吴晓凤董艺
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com