Unlock instant, AI-driven research and patent intelligence for your innovation.

Corn anti-oxidization active peptide and preparing method thereof

A technology of antioxidant activity and antioxidant peptides, applied in the field of food biology, can solve the problems of protein difficulty, low content of lysine and tryptophan, complex composition, etc., and achieve the effect of high antioxidant activity

Active Publication Date: 2018-08-28
QIQIHAR UNIVERSITY
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although corn flour is a natural plant protein raw material with abundant resources and low cost, it is obviously a non-complete nutritional protein because the content of lysine and tryptophan in it is not high.
In addition, the protein contained in corn yellow powder is difficult to dissolve in water, the composition is relatively complex, and the taste is very rough. Therefore, no matter in the food field or in the feed industry, few people care about it.
Even in many places, it is discharged naturally together with other by-products without recycling. This is not only a great waste of food resources, but also causes a certain degree of pollution to the ecological environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Corn anti-oxidization active peptide and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Preparation of enzymatic hydrolyzate of corn protein

[0035]Take a certain amount of maize yellow powder that has been extruded and expanded to remove starch, add a certain proportion of water to prepare a suspension with a substrate concentration of 10% (m / V), and first use alkaline protease Alcalase to carry out enzymolysis. The enzymolysis conditions are : Add 2% enzyme, enzymolysis temperature 50°C, pH7.7, time 75min; then add flavor protease Flavourzyme for synergistic enzymolysis, enzymolysis conditions are: add enzyme amount 5%, enzymolysis temperature 53°C, pH6.4 , time 50min. After hydrolysis, the enzymatic solution was heated at 100°C for 10 minutes to inactivate protease activity. The enzymolyzate was centrifuged at 4000r / min for 10min to discard the precipitate, and the obtained supernatant was a mixture of antioxidant active peptides.

[0036] 2. Ultrafiltration of corn protein hydrolyzate

[0037] The corn protein enzymatic hydrolyzate was subjected...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of food biology, and particularly relates to a corn peptide and a preparing method thereof. The corn peptide has the amino acid sequence of KSCAPLAS. The corn peptide has high anti-oxidization activity; it is detected that the IC50 value for removing DPPH free radicals by the corn peptide is 0.13 mg / mL and the IC50 value for removing superoxide anion free radicals by the corn peptide is 0.41 mg / mL. The invention further provides the preparing method of the corn peptide. The method includes the steps of obtaining a polypeptide mixture with anti-oxidization activity with extruded corn yellow powder without starch as the raw material through a protease collaborative enzymolysis method, collecting high-activity components with the molecular weight of 600-1400 Da stage by stage through ultrafiltration, ion-exchange column chromatography, gel chromatography and reversion phase chromatography, obtaining the amino acid sequences of six corn peptidesthrough mass spectrometry sequencing, and evaluating in-vitro anti-oxidization activity of the corn peptide after chemical synthesis to obtain the corn peptide with the amino acid sequence of KSCAPLAS.

Description

[0001] This application is a divisional application based on the Chinese patent application with the application number "CN201510565561.0" and the invention title "Corn Antioxidant Active Peptide and Its Preparation Method". The entire content of this application is derived from the records of the application documents of the parent case. technical field [0002] The invention relates to three new corn peptides with high antioxidant activity and a preparation method, belonging to the field of food biotechnology. Background technique [0003] Oxidation reactions play an important role in the generation of diseases, such as cancer, aging, arteriosclerosis, cardiovascular disease, diabetes, Alzheimer's disease, etc. These negative health conditions are attributed to oxidative damage to cellular components such as cell membranes, lipoproteins, enzymes, and nucleic acids. In food, oxidation reactions can also directly affect food quality, often associated with changes in the fla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/02C07K1/36C07K1/34C07K1/20C07K1/18
Inventor 刘晓兰郑喜群王晓杰金杜欣
Owner QIQIHAR UNIVERSITY