Preparation method of D-pantoic acid inner ester
A pantothenolactone and pantothenic acid technology, applied in the field of biosynthesis, can solve the problems of high VOC emissions, difficult wastewater treatment, raw material toxicity, etc., and achieve the effects of low cost, easy conversion process, and good product quality
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[0012] The embodiment of the present invention provides a preparation method of D-pantolactone, the preparation method at least includes the following steps:
[0013] (1) Under the action of catalase and α-ketoisovalerate reductase, valine is subjected to enzymatic conversion to obtain α-ketoisovalerate;
[0014] (2) reacting the α-ketoisovaleric acid and tetrahydrofolate under the action of hydroxymethyltransferase and magnesium chloride to prepare ketopantoic acid;
[0015] (3) performing enzymatic conversion of the ketopantoate under the action of α-ketoisovalerate reductase to obtain pantoate;
[0016] (4) preparing the pantoate to obtain the pantoate lactone.
[0017] Compared with the prior art, in the preparation method of D-pantolactone provided by the present invention, cheap and easy-to-obtain valine is catalyzed and converted by a biological enzymatic method to prepare pantoic acid. The invention has simple operation process, mild conversion conditions, high produ...
Embodiment 1
[0036] This implementation provides a kind of preparation method of D-pantolactone, and described preparation method comprises the following steps:
[0037] (1) Put 58.8g of valine into 850mL of water, control the temperature within the range of 25-40°C, use dilute sulfuric acid and sodium hydroxide to dissolve and adjust the pH value to 4.0-9.0, and simultaneously put 2.5g of valine into the feed liquid Catalase and 3g of α-ketoisovalerate reductase, carry out enzymatic conversion reaction, the pH value is 4.0-9.0 during the reaction, transform 2 hours and take samples to detect the generation amount of α-ketoisovalerate and the content of valine Residual amount, when the remaining valine is less than 3%, the reaction is considered to be complete, the reaction is terminated, and the α-ketoisovaleric acid feed liquid is obtained by filtration;
[0038] (2) The α-ketoisovaleric acid feed solution obtained in step (1) is warmed up to 60-75° C., and after being incubated for 30 m...
Embodiment 2
[0042] This implementation provides a kind of preparation method of D-pantolactone, and described preparation method comprises the following steps:
[0043](1) Ketopantoic acid can also be prepared by a chemical method, which is prepared by reacting isobutyraldehyde, formaldehyde and diethyl oxalate: in sodium methoxide solution, add diethyl oxalate and isobutyraldehyde under stirring at room temperature Butyraldehyde, after stirring and reacting for 2 hours, heat up to 25-35°C, add sodium hydroxide or triethylamine solution dropwise, continue to react for 4-5 hours, then control the temperature at 30-40°C, add dropwise an equimolar amount of sodium methoxide 37wt% formaldehyde solution, stirred and reacted for 6-15 hours, cooled to 10°C, acidified with sulfuric acid, kept at temperature above 30°C for no less than 2 hours, removed methanol and generated salt, adjusted pH with ammonia water or sodium hydroxide , the residue is extracted with ethyl acetate, the ethyl acetate is...
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