Fraction B of Sargassum Heinzii Ethyl Acetate Extract and Its Preparation Method and Application in Biocontrol of Marine Fouling
A technology of ethyl acetate extraction and Sargassum Heinz, applied in the directions of botanical equipment and methods, chemicals for biological control, applications, etc., to achieve the effects of great promotion potential, high safety and wide sources
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Embodiment 1
[0022] Sargassum heinzii ethyl acetate extract fraction B is extracted from Sargassum heinzii, and its separation and extraction steps include:
[0023] (1) Sargassum henslowianum C.Agardh was extracted three times with a 95% ethanol aqueous solution by volume fraction, and the extracts were combined to obtain an ethanol extract, concentrated by vacuum rotary evaporation at 45° C., and ethanol was removed to obtain a paste extract;
[0024] (2) The paste extract was resuspended in water at a ratio of 6 ml per gram, extracted 3 times with an equal volume of petroleum ether, discarded the petroleum ether extraction components, and extracted the remaining water layer with an equal volume of ethyl acetate for 3 times, the combined ethyl acetate extract was concentrated and dried to obtain the ethyl acetate extract;
[0025] (3) Carry out chromatographic separation of the ethyl acetate extract in an atmospheric silica gel column (silica gel 8000g, column length 180cm, column diamet...
Embodiment 2
[0028]Experimental group: Dissolve component B of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction B of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.
[0029] Control group: add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), and distribute it evenly on the bottom of the petri dish, and then add 13ml of seawater after volatilization is complete.
[0030] Blank group: add 13ml ...
Embodiment 3
[0036] Experimental group: Dissolve component B of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of Fraction B of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.
[0037] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), so that the solvent is evenly distributed on the bottom of the petri dish, and after the solvent is completely evaporated, add 13ml of seawater.
[0038]...
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