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Fraction B of Sargassum Heinzii Ethyl Acetate Extract and Its Preparation Method and Application in Biocontrol of Marine Fouling

A technology of ethyl acetate extraction and Sargassum Heinz, applied in the directions of botanical equipment and methods, chemicals for biological control, applications, etc., to achieve the effects of great promotion potential, high safety and wide sources

Inactive Publication Date: 2020-12-08
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on whether Sargassum henzii contains active ingredients against larvae attachment and spore germination of marine fouling organisms and whether it can be applied to the field of marine fouling biocontrol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Sargassum heinzii ethyl acetate extract fraction B is extracted from Sargassum heinzii, and its separation and extraction steps include:

[0023] (1) Sargassum henslowianum C.Agardh was extracted three times with a 95% ethanol aqueous solution by volume fraction, and the extracts were combined to obtain an ethanol extract, concentrated by vacuum rotary evaporation at 45° C., and ethanol was removed to obtain a paste extract;

[0024] (2) The paste extract was resuspended in water at a ratio of 6 ml per gram, extracted 3 times with an equal volume of petroleum ether, discarded the petroleum ether extraction components, and extracted the remaining water layer with an equal volume of ethyl acetate for 3 times, the combined ethyl acetate extract was concentrated and dried to obtain the ethyl acetate extract;

[0025] (3) Carry out chromatographic separation of the ethyl acetate extract in an atmospheric silica gel column (silica gel 8000g, column length 180cm, column diamet...

Embodiment 2

[0028]Experimental group: Dissolve component B of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of fraction B of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.

[0029] Control group: add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), and distribute it evenly on the bottom of the petri dish, and then add 13ml of seawater after volatilization is complete.

[0030] Blank group: add 13ml ...

Embodiment 3

[0036] Experimental group: Dissolve component B of the ethyl acetate extract of Sargassum henzii in a mixed solvent of chloroform and methanol (the mixed solvent of chloroform and methanol is formed by mixing chloroform and methanol at a volume ratio of 3:1), and the prepared concentration is 282.6 μg / ml The solution. Add 1 ml of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the dose of Fraction B of the ethyl acetate extract of Sargassum Heinzii coated on the bottom of the petri dish is 10 μg / cm 2 . Then 13ml of seawater was added to the Petri dish.

[0037] Control group: Add 1ml of chloroform-methanol mixed solvent (chloroform-methanol mixed solvent is a mixture of chloroform and methanol at a volume ratio of 3:1), so that the solvent is evenly distributed on the bottom of the petri dish, and after the solvent is completely evaporated, add 13ml of seawater.

[0038]...

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PUM

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Abstract

The invention discloses an ethyl acetate extract separated component B of Sargassum henslouianum and its preparation method and application in marine fouling biological prevention and removal. The ethyl acetate extract separated component B of Sargassum henslouianum in a low dosage coats the surface of the solid and has an obvious effect of inhibiting the attachment of the barnacle and the mussellarvae, and thus the ethyl acetate extract separated component B can be used for preparation of a marine fouling biological prevention and removal agent. The ethyl acetate extract separation componentB of Sargassum henslouianum is selected from Sargassum henslouianum, is a natural organic compound, does not pollute the water environment, is transported through the food chain to cause its enrichment in the organism, is environmentally friendly and safe, effectively inhibits the attachment of marine organisms, does not contain heavy metal elements such as copper and tin, has good social benefits from the perspective of environmental protection, and has a wide range of sources, is easy to separate and prepare, is suitable for large scale production, has a large promotion potential and has agood application prospect in marine fouling biological prevention and removal.

Description

Technical field: [0001] The invention belongs to the field of natural products, and in particular relates to the separation component B of ethyl acetate extract of Sargassum henzii and its preparation method and application in marine fouling biological control. Background technique: [0002] Marine fouling organisms refer to all kinds of organisms that fix or inhabit underwater parts of ships and artificial facilities and have adverse effects on human economic activities. The metal corrosion process causes corrosion and perforation; increases the dynamic load effect, causing man-made facilities to drift, unbalance or even overturn; competes with breeding objects for attachment substrates and bait, hinders their growth and development, and reduces the quality of aquatic products. Therefore, in the process of developing the marine economic industry, marine fouling biological control is always an indispensable and important part. [0003] Marine fouling organisms are composed ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/03A01P9/00C09D5/16
CPCA01N65/00A01N65/03C09D5/16
Inventor 章超桦严涛吴慧曹文浩卢虹玉韩帅帅谢恩义
Owner GUANGDONG OCEAN UNIVERSITY
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