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Labidura japonica de haan antimicrobial peptide DEI and application thereof in duck feed

An antimicrobial peptide and earwig technology, applied in the field of bioengineering, can solve problems such as duck production problems and epidemic diseases, and achieve the effect of strong killing effect and broad-spectrum antibacterial activity

Inactive Publication Date: 2018-09-07
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the process of raising ducks, epidemic diseases often appear, which brings great troubles to duck production

Method used

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  • Labidura japonica de haan antimicrobial peptide DEI and application thereof in duck feed
  • Labidura japonica de haan antimicrobial peptide DEI and application thereof in duck feed
  • Labidura japonica de haan antimicrobial peptide DEI and application thereof in duck feed

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Embodiment 1

[0028] In this embodiment, the Japanese earwig antimicrobial peptide DEI is extracted according to the following steps: Take 500 g of the adult body of the Japanese earwig, homogenize it in a high-speed tissue masher with 3% acetic acid aqueous solution for 5 minutes, place it in a refrigerator at 4°C overnight, centrifuge, and collect The supernatant; the precipitate was treated in the same way with 3% acetic acid aqueous solution. The two supernatants were combined, heated at 100°C for 1 h, left to stand in a refrigerator at 4°C, and filtered under reduced pressure. The filtrate was concentrated under reduced pressure and freeze-dried to obtain the extract of Japanese earwig. The Japanese earwig extract adopts chromatographic technology, uses D101 macroporous adsorption resin chromatographic column, and successively elutes with water and 55% ethanol, collects 55% ethanol eluate, recovers ethanol under reduced pressure, concentrates, and freeze-dries to obtain Japanese earwig...

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Abstract

The invention discloses a labidura japonica de haan antimicrobial peptide DEI and application thereof in duck feed, and belongs to the technical field of bioengineering. The labidura japonica de haanantimicrobial peptide DEI is prepared by acquiring an antimicrobial peptide DEI of labidura japonica de haan, testing an amino acid sequence of the antimicrobial peptide DEI by using an automatic protein sequencing instrument, further by means of genetic engineering, designing a cDNA (complementary deoxyribonucleic acid) fragment of the amino acid sequence, establishing cloning plasmid pUC57-TM, converting escherichia coli DH 5alpha, performing PCR (polymerase chain reaction) and sequencing to carry out further identification, establishing a recombinant expression vector pPICZaC-TM, performinglinearization on the recombinant expression vector, performing electric conversion into pichia pastoris SMD1168, screening to obtain a high-copy recombinant yeast conversion factor, and culturing a single colony of the recombinant yeast conversion factor, thereby obtaining the labidura japonica de haan antimicrobial peptide DEI. The labidura japonica de haan antimicrobial peptide DEI can be applied to duck feed, then the immunity of ducks can be improved, the morbidity of the ducks can be greatly reduced, the survival rate of the ducks can be increased, and thus the feeding cost of duck farmers can be reduced.

Description

technical field [0001] The invention relates to a Japanese earwig antimicrobial peptide DEI and its application in duck feed, belonging to the technical field of bioengineering. Background technique [0002] Antimicrobial peptides are a class of small molecular polypeptide substances with antibacterial activity produced by organisms, generally composed of 20-60 amino acid residues, with a molecular weight of about 2000-7000. Most of these active peptides have the characteristics of strong alkalinity, thermal stability and broad-spectrum antibacterial properties. The first antimicrobial peptide discovered in the world was a polypeptide with antibacterial activity produced by the chrysalis of the silkworm chrysalis, named Cercropins, in 1980 by Swedish scientist G. Boman et al. by injecting Bacillus cloacae and Escherichia coli. Initially, when people were studying the immune mechanism of the North American cerevisiae, they found that after the diapause pupae were induced by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435A23K20/147A23K50/75
CPCC07K14/43563A23K20/147A23K50/75
Inventor 冯兴军李仲玉毕重朋刘瑞奇张婷婷
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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