Application of AHL molecule to prevention and control of citrus bacterial canker as antibacterial synergist of chemical pesticide

A technology of citrus canker and chemical agents, which is applied in the field of xonopodispv.citri (Xa), can solve problems such as threats to human and animal health, environmental pollution, and soil compaction, and achieve the effects of promoting green agricultural development, environmental friendliness, and environmental protection

Active Publication Date: 2018-09-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Long-term single use of pesticides induces significant drug resistance of bacteria, making the amount of pesticides used in citrus production areas in my country far higher than the international average level. Excessive use of pest...

Method used

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  • Application of AHL molecule to prevention and control of citrus bacterial canker as antibacterial synergist of chemical pesticide
  • Application of AHL molecule to prevention and control of citrus bacterial canker as antibacterial synergist of chemical pesticide
  • Application of AHL molecule to prevention and control of citrus bacterial canker as antibacterial synergist of chemical pesticide

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Preparation of AHL Small Molecules

[0026] The seed solution of Pseudomonas aeruginosa cultured overnight was inserted into YEB medium, 28 o C. After culturing at 200 rpm for 24 hours, the culture supernatant was collected by centrifugation. The obtained supernatant is used for extraction of small molecules. Among them, the formula of YEB medium is: 0.5 g / LMgSO 4 •7H 2 O, 5 g / L Sucrose, 5 g / L Yeast Extract, 10 g / L Trypton, 5 g / L NaCl, pH 7.0.

[0027] The present invention adopts the conventional compound separation and extraction method to separate and extract the active substances in the fermentation broth. After collecting the supernatant of the culture medium by centrifugation, add an equal volume of ethyl acetate to the supernatant, place it at 4°C for 2 hours, then shake it at 20°C for 1 hour, extract it with a separatory funnel, and collect the upper layer. Then, the collected upper layer was separated and evaporated with a rotary evaporator, and ...

Embodiment 2

[0028] Example 2 The bactericidal and synergistic effect of small-molecule AHL mixed with 1% shenzimycin on Xac of citrus canker

[0029] Colony counting statistical method (CFU method) was used to measure the bactericidal synergistic effect of different concentrations of small molecule AHL extracts on Shenzimycin: the small molecule AHL was prepared and diluted to a certain concentration with methanol as a solvent, and cultured overnight to OD 600 =1.0 of Xac Bacterial solution, add 1% Shenzimycin diluted 200 times 100mg / ml, set a treatment without small molecule AHL as a positive control, and then set a series of small molecules with concentration gradients (40μM, 80μM, 160μM, 320μM, 500μM) Treatment of Molecular AHL Mixed with Shenzimycin. All processed in 28 o C. After culturing at 200rpm for 24 hours, dilute 10 4 times, take 200 μL of the bacterial solution to smear the plate (the medium is LB), and place it in a constant temperature incubator at 28°C for cultivation...

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Abstract

The invention discloses application of a micromolecular compound generated from bacteria as a synergist of a chemical pesticidal bactericide. The synergist is 3-oxydodecamoyl homoserine lactone (AHL)produced from PA (pseudomonas aeruginosa) and can be extracted from fermentation broth of the PA or artificially chemically synthesized. A trace amount of the micromolecular compound (AHL) is mixed with various chemical pesticidal bactericides to achieve an obvious synergistic effect on Xac (xanthomonas axonopodis pv.citri) killing. According to the invention, antibacterial effects of various chemical pesticidal bactericides can be remarkably improved, the using amount and residues of chemical pesticides are greatly reduced, the cost can be reduced, the environment can be protected, the problem of increasingly serious chemical resistance of pathogenic bacteria is effectively alleviated and achievement of a chemical reduction index and green development of agriculture are facilitated.

Description

technical field [0001] The present invention relates to Pseudomonas aeruginosa Pseudomonas aeruginosa, PA AHL-produced small molecule as a novel synergist against various chemical pesticide fungicides in X. citri Xanthomonas axonopodis pv. citri ( Xac ), which belongs to the field of plant protection of biological control technology. Background technique [0002] Citrus canker is an important bacterial disease that endangers the world's citrus industry. It has occurred to varying degrees in more than 30 countries and regions. The disease is easy to spread and difficult to control. Xanthomonas axonopodis pv.citri is the main type of Xanthomonas citrus canker in China, and it is also the subject of plant quarantine at home and abroad. Its hosts include more than ten species of Rutaceae plants, which harm leaves, branches, fruits, etc. Bacterial pus is easily spread and re-infected by rainwater or agricultural operations, often causing a large-scale epidemic outbreak ...

Claims

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Application Information

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IPC IPC(8): A01N43/08A01N43/60A01P1/00C12P17/04C12R1/385
CPCA01N43/08A01N43/60C12P17/04
Inventor 常长青廖金星邓嘉茹徐领会刘冉淋章婉贤
Owner SOUTH CHINA AGRI UNIV
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