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Sulfydryl DNA-modified gold nano-rod, preparation method thereof, heavy metal mercury ion detection method and application of heavy metal mercury ion detection method

A technology of gold nanorods and detection methods, applied in the field of gold nanomaterials, can solve the problems of complicated process, expensive instruments, complicated experimental materials, etc.

Active Publication Date: 2018-09-14
ANHUI NORMAL UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Such as the use of upconversion nanoparticles NaYF 4 : Fluorescence resonance energy transfer between Yb, Tm and gold nanorods to detect mercury ions, but the process is more complicated
The literature (S.J.Wu, N.Duan, Z.Shi, C.C.Fang, Z.P.Wang, Talanta, 128(2014) 327-336) uses dual fluorescence resonance energy transfer to detect lead ions and mercury ions, but the experimental materials of this detection method are complicated, Expensive instruments, low detection sensitivity and selectivity

Method used

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  • Sulfydryl DNA-modified gold nano-rod, preparation method thereof, heavy metal mercury ion detection method and application of heavy metal mercury ion detection method
  • Sulfydryl DNA-modified gold nano-rod, preparation method thereof, heavy metal mercury ion detection method and application of heavy metal mercury ion detection method
  • Sulfydryl DNA-modified gold nano-rod, preparation method thereof, heavy metal mercury ion detection method and application of heavy metal mercury ion detection method

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preparation example Construction

[0029] The invention provides a method for preparing gold nanorods modified with thiol DNA, comprising:

[0030] 1) Mix cetyltrimethylammonium bromide CTAB and chloroauric acid, then add sodium borohydride for contact reaction until the system turns yellowish brown to obtain seed liquid;

[0031] 2) Mix cetyltrimethylammonium bromide CTAB, silver nitrate, and chloroauric acid, and then add ascorbic acid for contact reaction until the system becomes colorless to obtain a growth solution;

[0032] 3) performing a contact reaction with the seed liquid and the growth liquid to obtain a gold nanorod GNRs solution;

[0033] 4) Incubate and post-treat the thiol DNA with the gold nanorod GNRs solution to obtain the thiol DNA-modified gold nanorod GNRs-ssDNA-2.

[0034] In step 1) of the present invention, the amount of each material can be selected in a wide range, but in order to improve the yield of gold nanorods, the preparation rate and the performance of the prepared thiol DNA m...

Embodiment 1

[0061] 1) Preparation of seed solution: 5.00mL 60°C CTAB (0.200mol / L) solution and 5.00mLHAuCl 4 (5.00×10 -4 mol / L) solution was mixed under constant stirring, and then quickly added 0.600mL of NaBH at 0-4°C 4 (1.00×10 -2 mol / L) solution, the solution changed from colorless to yellowish brown, stirred rapidly for 2 minutes and stored in a 30°C water bath for later use. Among them, CTAB, HAuCl 4 and NaBH 4 The solvent of the solution is water.

[0062] 2) Preparation of growth solution: 80.0 μL of AgNO 3 (4.00×10 -3 mol / L) solution, 5.00mL HAuCl 4 (1.00×10 -3 mol / L) solutions were mixed sequentially, and then 70.0 μL ascorbic acid solution (7.88×10 -2 mol / L) with continuous stirring, the color of the solution changed from yellow to colorless, and it was also stored in a water bath at 30°C. Wherein, the solvent of CTAB, silver nitrate, chloroauric acid, ascorbic acid solution is water.

[0063] 3) After 0.5h, use a pipette to absorb 12.0μL seed solution and add it to ...

Embodiment 2

[0066] 1) Preparation of seed solution: mix 10mL 60°C CTAB (0.200mol / L) solution with 6mL HAuCl 4 (5.00×10 - 4 mol / L) solution was mixed under constant stirring, then quickly added 0.700mL NaBH at 0-4℃ 4 (1.00×10 -2 mol / L) solution, the solution changed from colorless to yellowish brown, stirred rapidly for 2 minutes and stored in a 30°C water bath for later use. Among them, CTAB, HAuCl 4 and NaBH 4 The solvent of the solution is water.

[0067] 2) Preparation of growth solution: 10 mL of CTAB (0.200 mol / L) solution at 60°C and 87 μL of AgNO 3 (4.00×10 - 3 mol / L) solution, 6mL HAuCl 4 (1.00×10 -3 mol / L) solutions were mixed sequentially, and then 71 μL of ascorbic acid solution (7.88×10 -2 mol / L) with continuous stirring, the color of the solution changed from yellow to colorless, and the solution was also stored in a 30°C water bath. Wherein, the solvent of CTAB, silver nitrate, chloroauric acid, ascorbic acid solution is water.

[0068] 3) After 0.5h, use a pipe...

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Abstract

The invention discloses a sulfydryl DNA-modified gold nano-rod, a preparation method of the sulfydryl DNA-modified gold nano-rod, a heavy metal mercury ion detection method and the application of theheavy metal mercury ion detection method. The preparation method of the sulfydryl DNA-modified gold nano-rod comprises the steps that (1) CTAB and chloroauric acid are mixed, then sodium borohydride is added to conduct a contact reaction until the system becomes tawny, and then a seed solution is obtained; (2) CTAB, silver nitrate and chloroauric acid are mixed, ascorbic acid is added to conduct acontact reaction until the system becomes colorless, and a growth solution is obtained; (3) the seed solution and the growth solution are made to conduct a contact reaction, so that a gold nano-rod GNRs solution is obtained; (4) incubation and post-treatment are conducted on sulfydryl DNA and the gold nano-rod GNRs solution so that the sulfydryl DNA-modified gold nano-rod GNRs-ssDNA-2 is obtained. The sulfydryl DNA-modified gold nano-rod and Hg ions can form a T-Hg2+-T sandwich type structure so that a Cy5 nucleotide-gold nano-rod fluorescence resonance energy transfer system can be established to achieve Hg2+ detection.

Description

technical field [0001] The invention relates to gold nanomaterials, in particular to a sulfhydryl DNA-modified gold nanorod, a preparation method thereof, a detection method and application of heavy metal mercury ions. Background technique [0002] At present, environmental pollution has become the focus of attention of people, the country and even the whole world. Today's industrial pollution is serious, especially heavy metal pollution. Trace amounts of heavy metals can cause physical diseases. Hg(II), as one of the heavy metal ions, can be converted into methylmercury through the methylation of microorganisms deposited in water. Mercury ions in the environment, water and food accumulate through the blood circulation. When the concentration of mercury ions in the body reaches 25nmol / L, it is enriched in the human body through the food chain and has varying degrees of toxicity to many organs such as the heart, kidney, brain, stomach and intestinal tract. . The U.S. Envir...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B82Y30/00B82Y40/00G01N21/31
CPCG01N21/31B82Y30/00B82Y40/00B22F9/24B22F1/102
Inventor 陈红旗许发功王丽
Owner ANHUI NORMAL UNIV
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