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A kind of inactivated vaccine for preventing and treating novel goose astrovirus and preparation method thereof

A technology of astroviruses and inactivated vaccines, applied in biochemical equipment and methods, viruses, antiviral agents, etc., can solve the problems of ineffective antiviral and antibacterial treatment methods, lower pass rate of goose slaughter, and economical problems of goose breeding industry. Loss and other issues, to achieve the effect of good commercial development prospects, good safety, and effective immune protection

Active Publication Date: 2020-06-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Severe urate deposition occurred in the body cavity and joints of the sick goose, which caused the gosling to grow slowly, increase the feed-to-meat ratio, and significantly reduce the pass rate of goose slaughter, causing serious damage to the goose breeding industry. Economic losses
[0004] Research has found that the outbreak of the disease is caused by a new type of goose star virus infection, and there is no vaccine to prevent it; conventional antiviral and antibacterial treatments are ineffective

Method used

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  • A kind of inactivated vaccine for preventing and treating novel goose astrovirus and preparation method thereof
  • A kind of inactivated vaccine for preventing and treating novel goose astrovirus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] In the preparation method of the above-mentioned inactivated vaccine, GKC cells are used to cultivate the virus. This cell line has very good susceptibility to the novel goose astrovirus of the present invention, can be continuously passed down, and can also be cultured in suspension. Virus multiplication and vaccine production.

[0036] Adjuvants refer to substances that can effectively promote, enhance or prolong the body's specific immune response to antigens after being added to vaccine preparations. The selection of adjuvants is the basis of the vaccine emulsification process, and a stable vaccine emulsion can effectively improve the ability of animals to resist diseases. Therefore, choosing the appropriate adjuvant and determining the optimum ratio of water phase and oil phase are of great significance to the stability of the vaccine.

Embodiment 1

[0039] Example 1: Discovery and Identification of Novel Goose Astrovirus

[0040] 1. Materials and methods

[0041] 1.1 Collection and processing of disease materials:

[0042] Take liver, spleen, kidney and other tissues of dead goslings, add sterile saline at a ratio of 1:4 for homogenization, place the homogenate in the refrigerator for 3 times, centrifuge at 6000r / min for 15min, and take the supernatant The solution was filtered through a 0.22 μm filter and stored at -20°C for later use.

[0043] 1.2 Main reagents:

[0044] Goose embryos were purchased from a goose farm in Liaocheng, Shandong, the RNA extraction kit was purchased from Beijing Kangwei Century Co., Ltd., the ordinary agarose gel recovery kit was purchased from Beijing Quanshijin Company, and the reverse transcription kit was purchased from Bao Biology (Dalian ) Co., Ltd., 2×EsTaq MasterMix was purchased from Beijing Kangwei Century Co., Ltd., and DL2000Marker was purchased from Bao Biological (Dalian) Co....

Embodiment 2

[0066] Embodiment 2: the preparation of inactivated vaccine

[0067] (1) Virus propagation and harvest:

[0068] The isolated and identified novel goose astrovirus was inoculated into well-growing GKC cells, and the virus was proliferated in large quantities to obtain sufficient virus liquid. The obtained sufficient amount of cell virus liquid was frozen at -20°C, and after two freezing and thawing, the cell virus liquid was collected.

[0069] (2) Purification of virus:

[0070] Use established detection methods such as PCR and RT-PCR to detect whether other common viruses are contained in the obtained virus liquid. The detection items include avian influenza virus (AIV), Newcastle disease virus (NDV), goose parvovirus (GPV), infectious bronchitis virus (IBV), infectious bursal virus (IBDV), etc., and through the use of corresponding antibodies and The virus liquid is neutralized and the seed virus is purified.

[0071] (3) Inactivation of virus liquid:

[0072] The viru...

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Abstract

The invention discloses an inactivated vaccine for preventing and treating novel goose astroviruses and a preparation method thereof. The inactivated vaccine is prepared from the effective dose of inactivated goose astrovirus strain virus liquid in prevention or treatment. A preservation number of the goose astrovirus strain is CCTCC NO: V201808. The prepared novel goose astrovirus inactivated vaccine is good in safety, any local or general adverse effects caused by the vaccine do not appear, and the detection of each index is stable and effective. After the inactivated vaccine is used for immunizing a young goose, the young goose can acquire the higher antibody level, and a persistent period is long. The infection and outbreak of the novel goose astroviruses can be prevented and treated specifically, and the effective immune protection is provided for a goose group. In addition, a production technology for the vaccine is simplified, and the large-scale production can be realized.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an inactivated vaccine for preventing and treating novel goose astrovirus and a preparation method thereof. Background technique [0002] Astrovirus (AStV) is a kind of non-encapsulated, spherical, single-stranded positive-sense RNA virus with a diameter of 28-30nm. The genome is infectious and 6.4-7.9kb long. The infection of this virus has a certain prevalence. It can cause enteritis and diarrhea in humans or animals, sometimes accompanied by vomiting and abdominal pain. Astroviridae can be divided into mammalian Astrovirus genus and avian Astrovirus genus according to the different infected hosts. Avian Astrovirus can cause a variety of diseases in poultry. vary with each other. Goose astrovirus (GAstV), which mainly affects young goslings, has been prevalent in many provinces, increasing the mortality rate of geese, and greatly endangering the healthy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/14A61P19/06
CPCA61K39/12A61K2039/5252A61P19/06A61P31/14C12N2770/12034
Inventor 刁有祥唐熠杨晶
Owner SHANDONG AGRICULTURAL UNIVERSITY
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