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Detection method for micro plastic in aquatic products

A detection method and technology for aquatic food, which are applied in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of difficulty in microplastics, increased difficulty in detection, lack of pretreatment methods, etc., and achieve the effect of easy counting

Inactive Publication Date: 2018-10-02
中国人民解放军火箭军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As a new type of pollutant, most people do not have a clear understanding of microplastics. Therefore, the current detection and analysis of microplastics mainly focuses on the detection of seawater and seafood, and less detection of freshwater aquatic products. It is mainly microplastics with small particle size and irregular shape. It is more difficult to label irregular microplastics with fluorescent light, which makes the detection more difficult. There is a lack of an efficient pretreatment method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Preparation 1 of fluorescent material

[0025] 20 parts by weight of fluorescent powder, 4 parts by weight of methylcellulose, 3 parts by weight of benzoic acid, 1 part by weight of polyacrylamide, 8 parts by weight of konjac glucomannan, the particle diameter of the fluorescent powder is 0.3mm;

[0026] Mix fluorescent powder and methylcellulose, dissolve in pure water and heat to 60°C in a water bath, stir for 10 minutes, add benzoic acid and mix well, continue to heat in a water bath to 90°C, add polypropylene at a stirring speed of 300r / min Amide, adjust the pH of the solution to 8, add konjac glucomannan, stir at 2500r / min for 30min, dry and grind it into powder, and screen the powder with a particle size of 0.4-0.7mm to obtain the fluorescent material.

Embodiment 2

[0027] Embodiment 2: Preparation 2 of fluorescent material

[0028] 25 parts by weight of fluorescent powder, 6 parts by weight of methylcellulose, 4 parts by weight of benzoic acid, 2 parts by weight of polyacrylamide, 12 parts by weight of konjac glucomannan, the particle diameter of the fluorescent powder is 0.5mm;

[0029] Mix the fluorescent powder and methyl cellulose, dissolve in pure water and heat in a water bath to 50°C, stir for 15 minutes, add benzoic acid and mix well, continue to heat in a water bath to 70°C, add polypropylene at a stirring speed of 300r / min Amide, adjust the pH of the solution to 9, add konjac glucomannan, stir at 3500r / min for 45min, dry and grind it into powder, and screen the powder with a particle size of 0.4-0.7mm to obtain the fluorescent material.

Embodiment 3

[0030] Embodiment three: to the detection of freshwater fish carp

[0031] Fluorescent material is selected from embodiment one

[0032] Carp sample collection: Carp samples were loaded with stainless steel fishing nets and glass bottles washed three times and dried;

[0033] Fluorescent labeling of carp samples: culture the collected carp samples in the culture solution containing fluorescent materials, the culture solution is prepared according to the ratio of fluorescent material volume: pure water volume = 1:1000, no feeding is carried out during the period, after 2 days of cultivation, use pure Wash the surface three times with water, and collect the culture fluid and cleaning fluid as sample 1 to be tested;

[0034] Carp sample processing: Dissect the cleaned carp samples and separate them into viscera, gills and fins, scales, tail, head, and skin; fish meat and bones are processed separately; viscera, gills, fins, scales, tail, head, Dry the skin at 50°C for 5 hours a...

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PUM

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Abstract

The invention relates to a detection method for micro plastic in aquatic products. The detection method comprises the steps as follows: caught aquatic products are placed in a culture solution containing a fluorescent material for culture, dissection is performed, obtained internal organs and epidermis are subjected to enzymolysis, obtained trunks are digested, then, the fluorescent material is used for fluorescence labeling, and detection, counting and calculation are performed. The fluorescent material is prepared from raw materials in parts by weight as follows: 20-25 parts of phosphor withthe particle size of 0.3-0.5 mm, 4-6 parts of methylcellulose, 3-4 parts of benzoic acid, 1-2 parts of polyacrylamide and 8-12 parts of konjac glucomannan. The method is mainly used for detecting micro plastic in freshwater fishes and freshwater shrimps, the pretreatment of samples is added, micro plastic screening and analysis calculation are performed on different parts, the fluorescence labeling capacity for the micro plastic is enhanced, and observation is easy.

Description

technical field [0001] The invention relates to the technical field of food detection methods, in particular to a detection method for microplastics in aquatic foods. Background technique [0002] Plastic products are widely used in today's society, and its annual output worldwide has exceeded 3×10 8 t, and is taking 0.2×10 8 t·a -1 speed growth. Due to mass production and use, plastic products will inevitably enter the water environment. It is estimated that there are more than 2.5×10 5 tPlastic waste. After physical degradation, photodegradation and biodegradation, these plastic wastes will be further decomposed to form microplastics with a particle size of less than 5mm. There are many types of microplastics, which are divided by material. The microplastics detected in the environment mainly include polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), polyester (PEst ) and polyethylene terephthalate (PET); in terms of shape, it is divi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 屈明玥宋振耀陈钢邱洪杰刘全斌廖远祥
Owner 中国人民解放军火箭军特色医学中心
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