Method used for preparing 6'-acetyl ginsenoside F1

Abstract

The invention discloses a method used for preparing 6'-acetyl ginsenoside F1. The method comprises following steps: 1, ginseng buds are collected, smashed, and are subjected to heat backflow extraction with 95% ethanol, and concentration into paste is carried out so as to obtain a total extracted product; 2, the total extracted product is prepared into a solution with a concentration of 0.2g crudedrug / ml with 25% ethanol, filtering is carried out, and column chromatography is carried out, wherein sample injection amount is 0.8BV, LX-68 macroporous adsorption resin column column bed diameter height ratio is controlled to be 1:9, 3BV of 40% ethanol is adopted for impurity removing, 9BV of 75% ethanol is adopted for elution, 8 to 9 BV of 75% ethanol eluate is collected, pressure reduced concentration is carried out until no ethanol is detected, and then freeze-drying is carried out so as to obtain a crude freeze-dried powder; and 3, high speed counter current separation is carried out, wherein n-butanol / ethyl acetate / methanol / water / chloroform solvent system ratio is controlled to be 1:18:1:18:0.5, the elution fractions corresponding to 6'-acetyl ginsenoside F1 are collected based ona chromatogram, and concentration is carried out so as to obtain 6'-acetyl ginsenoside F1.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a method for preparing ginsenoside F1 derivatives. The ginsenoside F1 derivatives include 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1 and 6'-malonylformyl Ginsenoside F1. Background technique [0002] Previous studies have found that 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 have excellent tyrosinase inhibitory activity and can be used to prepare whitening cosmetics. From the chemical structure point of view, 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 are derivatives of ginsenoside F1, but ginsenoside F1 is a derivative of tyrosine The inhibitory activity of the enzyme is extremely weak, far less than that of its derivatives. [0003] At present, the separation and preparation of 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 must rely on re...

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Problems solved by technology

From the chemical structure point of view, 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 are derivatives of ginsenoside F1, but ginsenoside F1 is a derivative of tyrosine Enzyme inhibitory activity is extremely weak, far inferior to its derivatives

[0003] At present, the separation and preparation of 3-acetyl ginsenoside F1, 6'-acetyl ginsenoside F1, and 6'-malonylformyl ginsenoside F1 must rely on repeated silica gel column chromatography, however, repeated silica gel column chromatography dead adsorption Strong, large loss, not suitable for large-scale preparation at all

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