Paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of complex microbial agent thereof

A technology of bacillus and compound bacteria agent, applied in the direction of application, fungicide, bacteria, etc., can solve the problems of no high efficiency, low toxicity and no environmental pollution

Active Publication Date: 2018-10-12
DEZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no biological method with high efficiency, low toxicity and no environmental pollution for the control of pear brown spot disease

Method used

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  • Paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of complex microbial agent thereof
  • Paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of complex microbial agent thereof
  • Paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of complex microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Screening and Identification of Paenibacillus Lzh-N1 and Streptomyces circulans Lzh-48

[0030] Both the strain Lzh-N1 and the strain Lzh-48 were isolated from the rhizosphere soil of pear trees infected with brown spot disease in the orchard of the Institute of Fruit Trees, Shandong Academy of Agricultural Sciences, Tai'an City, Shandong Province. The specific method is as follows:

[0031] Screening of antagonistic microorganisms from plant rhizosphere soil by plate confrontation method. Take rhizosphere soil from the roots of infected plants and send it to the laboratory as soon as possible under low temperature conditions. Put the rhizosphere soil into sterile water for gradient dilution, and then spread it on LB solid medium and Gaoshi No. 1 solid medium, and pick a single colony after culturing at 28°C for 24 hours. Inoculate the obtained single colony on the plant pathogenic bacteria to see if it produces a zone of inhibition and whether it has antagon...

Embodiment 2

[0038] Antagonistic effect of Paenibacillus Lzh-N1 and Streptomyces circulans Lzh-48 in embodiment 2 of the present invention

[0039] The antagonism of Paenibacillus Lzh-N1 and Streptomyces circulans LZH-48 was detected by plate confrontation method. The pear tree brown spot pathogen Mycosphaerella sentina (Fr) Schrorter was inoculated on PDA solid medium (200g of peeled potatoes, 20g of sucrose, 20g of agar, 1000mL of distilled water, natural pH), cultured at 30°C for 48hrs and then set aside. Paenibacillus Lzh-N1 was inoculated onto beef extract peptone solid medium (beef extract (3.0g), peptone (10.0g), NaCl (5.0g), agar 20g, distilled water 1000mL), and Streptomyces circulans LZH-48 Inoculate into Gao's solid medium (soluble starch (20g), KNO 3 (1g), K 2 HPO 4 (0.5g), MgSO 4 ·7H 2 O (0.5g), NaCl (0.5g), FeSO 4 ·7H 2 O (0.01g), 20g of agar, 1000mL of distilled water, pH=7.4-7.6), cultured at 30°C for 48hrs, then inoculated to both sides of the pathogenic bacteria of...

Embodiment 3

[0040] Embodiment 3 contains Paenibacillus Lzh-N1 microbial fertilizer and preparation method thereof

[0041] Specific steps are as follows:

[0042] (1) Preparation of fermentation broth

[0043] The seed solution of Paenibacillus Lzh-N1 was inoculated in beef extract peptone liquid medium with a pH of 7.0, the inoculation amount was 6% (v / v), and fermented at 28°C and 200rpm for 48h to obtain Paenibacillus The fermentation broth of Lzh-N1;

[0044] (2) Preparation of microbial fertilizer

[0045] Mix the fermented liquid obtained in step (1) with the organic fertilizer in a volume-to-mass ratio of 1:12. After mixing evenly, carry out solid fermentation. The fermentation temperature does not exceed 50°C. - After 8 days, when the content of Paenibacillus Lzh-N1 reaches 1×10 8 When the cfu / g is above, the microbial fertilizer containing Paenibacillus Lzh-N1 can be obtained.

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Abstract

The invention relates to paenibacillus Lzh-N1 capable of preventing and treating pear brown spots and application of a complex microbial agent thereof. The paenibacillus Lzh-N1 is preserved in the common microbial center of the China microbial culture preservation management committee with the preservation number of CGMCC No.13455, the preservation date is December 15, 2016, and the address of thepreservation institution is No.3, Yard 1, Beichenxi Road, Chaoyang District, Beijing. The invention further discloses application of the paenibacillus Lzh-N1 and microbial fertilizer of a complex microbial agent of the paenibacillus Lzh-N1 and streptomyces globosus to prevention and treatment of pear brown spots. The paenibacillus Lzh-N1 can effectively prevent pear brown spots and improve the quality and output of pears, and has the effects of nontoxicity, high efficiency and non-environment pollution.

Description

technical field [0001] The present invention relates to a strain of Paenibacillus Lzh-N1 capable of preventing and treating brown spot disease of pear trees and the application of its compound bacterial agent, in particular to the application of Paenibacillus Lzh-N1 in the prevention and treatment of brown spot disease of pear trees, including Paenibacillus Lzh-N1. The application of the mixed bacterial agent of Bacillus Lzh-N1 and Streptomyces roundosa Lzh-48 in the prevention and treatment of pear tree brown spot belongs to the field of environmental microorganisms. Background technique [0002] my country's pear production accounts for about 2 / 3 of the world's total output, and its export volume accounts for about 1 / 6 of the world's export volume. Chinese pears play a pivotal role in the development of the world's pear industry. However, pear tree brown spot caused by the fungus Mycosphaerella sentina (Fr) Schrorter occurs in large numbers every year in major pear produci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C05G3/00A01N63/00A01N63/02A01P3/00C12R1/01
CPCA01N63/00A01N63/10C05F3/00C05F17/00C05G3/00C12N1/205C12R2001/01C05F11/08Y02W30/40
Inventor 李正华曾强成宋峰王吉华周耀旗于家峰冉坤沈亮
Owner DEZHOU UNIV
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