Check patentability & draft patents in minutes with Patsnap Eureka AI!

Micractinium sp. and applications thereof

A technology of Misantha, CCTCCM2016743, applied in the field of novel Misantha isolates, which can solve the problems of energy consumption, time-consuming, expensive, and increased concentration of algae

Active Publication Date: 2018-10-16
FOOD IND RES & DEV INST
View PDF13 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Taking the self-cultivation mode as an example, the self-cultivation of microalgae is mainly to suspend microalgae in the culture medium. However, the current culture technology is still unable to increase the concentration of algae in the culture medium to 10g / L (1 %)above
In addition, due to the low concentration of algae in the culture medium and the tiny cells of the algae, it takes complicated processes and equipment to separate the algae from the culture medium when the algae are recovered and dried, which is energy-consuming, time-consuming and expensive, making the algae The recycling cost of microalgae can account for 20-30% of the total production cost of microalgae biodiesel

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micractinium sp. and applications thereof
  • Micractinium sp. and applications thereof
  • Micractinium sp. and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] Materials and Methods

[0054] 1.C culture medium

[0055] Add Ca(NO 3 ) 2 4H 2 O 15mg, KNO 3 10mg, Disodium β-Glycerophosphate 5H 2 O 5mg, MgSO 4 ·7H 2 O 4mg, vitamin B12 0.01μg, biotin (Biotin) 0.01μg, thiamine (Thismine) HCl 1μg, PIV trace element solution 0.3mL and Tris 50mg, then the volume was replenished to 100mL, and the pH was adjusted to 7.5 before autoclaving bacteria. If it is a 1.5% (w / v) agaric solid medium, 15 g of agaric gum needs to be added for sterilization together.

[0056] The preparation of PIV trace element solution is to add Na in sequence 2 EDTA·2H 2 O 100mg, FeCl 3 ·6H 2 O 19.6mg, MnCl 2 4H 2 O 3.6mg, ZnCl 2 1.04mg, CoCl 2 ·6H 2 O 0.4 μg with Na 2 MoO 4 2H 2O 0.25 μg, followed by rehydration to 100 mL volume and autoclaving.

[0057] 2 Algae sample collection, isolation and cultivation

[0058] Take about 10 g of soil samples from the Alishan mountainous area in Chiayi, Taiwan, and place them in a 50 mL centrifuge tube,...

example 1

[0123] Example 1. Identification of algae strains

[0124] The 74C algae strain was isolated and purified from soil samples in the Alishan mountainous area of ​​Chiayi, Taiwan. Observing its morphology with a microscope (1,000X), it was found that the 74C algae strain existed as a single non-clustered algal body, in which there were no bristle-like protrusions outside the cell wall, and the cells were round. Depending on the number of days of algae culture, the cell diameter was about 3~6μm( figure 1 A). After staining with Nile Red, a large number of obvious and yellow oil droplets were observed inside the algae with a fluorescence microscope, indicating that oil droplets could accumulate in the algae ( figure 1 B).

[0125]DNA sequencing was performed on the 18S rDNA and ITS regions of the 74C strain, and a 1,744bp 18S rDNA sequence (SEQ ID NO:1) and a 674bp ITS sequence (SEQ ID NO:2) were obtained, respectively. After comparing the 18S rDNA and ITS sequences with the nr...

example 2 74

[0139] The culture condition of example two, 74C algae strain

[0140] (1) Cultivation temperature (heat resistance temperature) test

[0141] Figure 4 The results show that the 74C algae strain can grow sustainably and significantly on the C medium plate at a culture temperature of 30°C to 40°C. Accordingly, the 74C strain can grow in a temperature range of about 20°C to about 40°C, and can be thermotolerant in a temperature range of about 30°C to about 40°C.

[0142] (2) Medium pH value test

[0143] Figure 5 The results showed that the 74C strain could grow sustainably and significantly in the liquid C medium at pH 4 to pH 9.5. Accordingly, the 74C strain can grow in an environment of about pH 4 to about pH 9.5.

[0144] (3) Medium salinity test

[0145] Image 6 The results showed that the 74C algae strain had continuous and obvious growth in the liquid C medium with a salinity of 0% (w / v) to 4.0% (w / v). Thus, the 74C strain can grow in salinity environments from...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Calorific valueaaaaaaaaaa
Login to View More

Abstract

The invention relates to micractinium sp. and applications of the micractinium sp. The micractinium sp. contains the 18S rDNA sequence of the nucleotide sequence as shown in SEQ ID NO:1 and the ITS area sequence of the nucleotide sequence as shown in SEQ ID NO:2. The invention relates to the separated micractinium sp. capable of growing on different materials for forming the biological membrane and producing high amount of triglyceride. The invention further relates to the applications of the separated micractinium sp. in producing biomass fuel and edible oil.

Description

technical field [0001] The present invention relates to novel isolates of the genus Micractinium sp., which can grow and form biofilms on different materials, and can be cultivated in a biofilm culture mode to reduce the impact on water resources and land. demand, and simplify the harvesting process and reduce harvesting costs. The isolate can produce a high amount of triacylglycerol, can absorb carbon dioxide during cultivation, and can be used for carbon fixation, and has characteristics such as high calorific value, low ash content and low sulfur content, and can be used as a production plant. source of high-quality diesel and cooking oil. Background technique [0002] Since the Industrial Revolution in the 18th century, human demand for petrochemical energy has been increasing day by day. However, the natural reserves of petrochemical energy are limited, so it is gradually necessary to seek new alternative energy sources. The first generation of biomass energy uses foo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12P7/64C10G3/00C10L5/44C12R1/89
CPCC10G3/00C10L5/44C12P7/6409C12P7/6445C12P7/649C12N1/125C12R2001/89Y02E50/10Y02E50/30Y02P30/20
Inventor 俞铭诚涂景瑜廖丽玲
Owner FOOD IND RES & DEV INST
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com