Supercharge Your Innovation With Domain-Expert AI Agents!

fe 3+ Molecular fluorescence test agent and preparation method thereof

A testing agent, fluorescence technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as small Stokes shift, achieve high quantum yield, good test accuracy and sensitivity, good The effect of light stabilization

Active Publication Date: 2019-07-02
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to provide a kind of Fe 3+ Molecular fluorescence test agent and preparation method thereof, to solve the Fe in the prior art 3+ The problem of too small Stokes shift of molecular fluorescence testing reagent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • fe <sup>3+</sup> Molecular fluorescence test agent and preparation method thereof
  • fe <sup>3+</sup> Molecular fluorescence test agent and preparation method thereof
  • fe <sup>3+</sup> Molecular fluorescence test agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0038] According to another aspect of the present invention, there is also provided a Fe 3+ The preparation method of molecular fluorescence testing agent, it comprises the following steps:

[0039] In step S1, compound A and compound B are condensed to form compound C; the structures of compound A, compound B and compound C are as follows, wherein X in compound A is a halogen:

[0040]

[0041] Step S2, subjecting compound C to a hydrolysis reaction to obtain Fe 3+ Molecular fluorescence test reagent:

[0042]

[0043] As mentioned above, the Fe prepared by the modified preparation method of the present invention 3+ Molecular fluorescent test reagents have both better test accuracy and sensitivity. And in this preparation method, compound C is prepared by condensation reaction of compound A and compound B first, and then prepared by hydrolysis of compound C to obtain Fe 3+ Molecular fluorescence testing reagent, the reaction route is simple, the cost is low, and the...

Embodiment 1

[0068] Synthesis of compound 1

[0069]

[0070] 4-cyanobenzoic acid (80 g, 544 mmol) was dissolved in anhydrous CH 2 Cl 2 (1000mL). Add oxalyl chloride (104 mL, 816 mmol) and 10 mL of dimethylformamide (DMF). The resulting reaction mixture was stirred at room temperature for 1 hour until gas evolution ceased. Solvent was removed. The resulting residue was treated with 600 mL of a pyridine / tert-butanol mixture (1:1) and stirred at room temperature for 6 hours. The solvent was evaporated under reduced pressure, and the green residue was suspended in H 2 O middle. The aqueous suspension was extracted with ethyl acetate (3 x 500 mL). The combined organic layers were washed with 10% KHSO 4 (2×500mL), H 2 O(500mL)NaHCO 3 (500mL), H 2 O (500 mL) and brine (500 mL) washed. Solvent with Na 2 SO 4 Dry and evaporate. The crude product was purified by column chromatography using ethyl acetate / petroleum ether (1:4) to give white solid 58g (yield 52%).

[0071] Synthesis...

Embodiment 2

[0091] The technique of each step in this embodiment is the same as Example 1, and the only difference is: in the synthetic process of compound 1, adjust the consumption of oxalyl chloride, make the mol ratio of 4-cyanobenzoic acid and oxalyl chloride be 2:3, The volume ratio between pyridine and tert-butanol is 1.1:1. The yield of the final compound 1 was 49%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a Fe<3+> molecular fluorescence testing agent and a preparation method thereof. The Fe<3+> molecular fluorescence testing agent has a structure represented by a formula (I). Theprovided Fe<3+> molecular fluorescence testing agent takes 1,8-naphthalene imide as the fluorescence group, thus the light stability of the testing agent is good, the exciting light and emitting light are both long-wavelength visible light; the Stokes shift is large, and the quantum yield is high. At the same time, a group (represented in the description) of the testing agent is highly sensitiveto Fe<3+>; nitrogen of pyridine rings and 4-nitrogen of amino group can carry out complexing with Fe<3+> to form ion complex, electron transferring or energy transferring can be triggered, and thus the testing agent generates different fluorescence responses at different Fe<3+> concentrations. The provided Fe<3+> molecular fluorescence testing agent thus has better accuracy and sensitivity in tests.

Description

technical field [0001] The invention relates to the technical field of organic synthesis and elemental analysis, in particular to a Fe 3+ Molecular fluorescence test agent and its preparation method. Background technique [0002] Iron is one of the most abundant trace elements in the body. It is an important component of hemoglobin, myoglobin and various enzymes, and participates in processes such as oxygen uptake, oxygen metabolism, and electron transfer. If the body lacks iron, it can affect the synthesis of hemoglobin, and reduce the activity of enzymes such as cytochrome c, ribonucleotide reductase, and succinate dehydrogenase, resulting in serious body dysfunction. Fe in human body 3+ Changes in content are associated with many diseases, such as iron deficiency can lead to anemia, cancer, diabetes and organ dysfunction, etc., while iron excess can induce Alzheimer's disease, Huntington's disease and Parkinson's disease through the generation of reactive oxygen species...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D401/12C09K11/06G01N21/64
CPCC07D401/12C09K11/06C09K2211/1029G01N21/6428G01N2021/6432
Inventor 戴宇赵建永张文昕
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com