Bacillus megaterium strain ZT-P and application thereof

A technology of ZT-P and bacterial strains, which is applied in the field of agricultural microorganisms, can solve problems such as insufficient activity and poor adaptability, and achieve the effects of wide application range, strong adaptability, and improved phosphorus dissolution rate

Active Publication Date: 2018-10-19
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus-dissolving fungi: Aspergillus, Penicillium, but the existing phosphorus-dissolving bacteria have problems such as insufficient activity and poor adaptability

Method used

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  • Bacillus megaterium strain ZT-P and application thereof
  • Bacillus megaterium strain ZT-P and application thereof
  • Bacillus megaterium strain ZT-P and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening and identification of phosphate solubilizing bacteria ZT-P

[0033] Medium formula: LB Trptone 10g, Yeast extract 5g, NaCl 10g, solid medium add 15g-18g agar powder, distilled water to dilute to 1000mL. 121°C, 20min.

[0034] PVK medium: Glucose 10g, Ca 3 (PO 4 ) 2 5g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.2g, MgSO 4 0.1g, KCl 0.2g, yeast extract 0.5g, MnSO 4 0.002g, FeSO 4 0.002g, 0.4% bromophenol blue 6mL, distilled water dilute to 1000mL. 121°C, 20min.

[0035] Monkinna solution inorganic phosphorus medium: Glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, KCl 0.3g, NaCl 0.3g, MgSO 4 ·7H20 0.3g, FeS0 4 ·7H 2 O0.03g, MnS0 4 ·4H 2 O 0.03g, Ca 3 (PO 4 ) 2 (Inorganic Phosphorus Source) 10g, pH7.0~7.5 solid medium, add 15g~18g agar powder, distilled water to make the volume to 1000mL. 121°C, 20min. Monkinna solution organic phosphorus medium: Glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, KCl 0.3g, NaCl 0.3g, MgS0 4 ·7H 2 00.3g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 ·4H 2 O 0.03g, soft phospholi...

Embodiment 2

[0052] Example 2 Germination test of the supernatant of the phosphate solubilizing bacteria ZT-P strain shake flask

[0053] Surface disinfection of plant seeds: select plump rice seeds, rinse them thoroughly with tap water, and then rinse with distilled water; soak in 75% ethanol for 3 minutes, discard 75% ethanol, soak in 1.6% sodium hypochlorite for 3 minutes, and wash with sterile water for 5~ 10 times, until there is no smell of sodium hypochlorite.

[0054] The phosphate-dissolving bacteria were inserted into 100 / 250mL LB medium according to the inoculation amount of 1% and placed in a constant temperature shaker at 37°C and 180rpm for 3D culture; the obtained fermentation product was centrifuged at 10000rpm for 10min, and the supernatant was reserved for future use . The effect of fermentation products on the germination of rice seeds (germination rate, germination vigor, root length, bud length): There are 3 treatments in this experiment, and 3 replicates for each treatmen...

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Abstract

The invention discloses a Bacillus megaterium strain ZT-P and application thereof. The strain ZT-P is Bacillus megaterium, has the property of dissolving with organophosphorus and inorganic phosphorusbacteria, and was preserved in CGMCC on April, 2018, with a strain preservation number of CGMCC NO.15602. The strain ZT-P provided by the invention has high vitality and adaptability, and can be widely used for fertilizer and pesticide reducing treatment of microbial fertilizer. By tests, the phosphate solubilization capacity of the microbial fertilizer prepared from the strain ZT-P in soil during flask shaking at the 5th day reaches maximum 156.7 [mu]g / mL. Compared with the phosphate solubilization rate before mutagenesis, the phosphate solubilization rate of the strain is improved by 20 percent, and dissolution rates of organophosphorus and inorganic phosphorus in the soil are reduced. Therefore, according to the prepared microbial organic fertilizer, dissolution periods of the organophosphorus and the inorganic phosphorus are shortened, and dissolved quantities of the organophosphorus and the inorganic phosphorus are reduced.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a Bacillus megaterium strain ZT-P capable of dissolving inorganic organic phosphorus and its application in soil fertilizer. Background technique [0002] Modern agricultural production places great demands on the versatility and high efficiency of chemical fertilizers. With the continuous development of science and technology, the use of chemical fertilizers has increased year by year, and the impact on farming soil has also become greater. The use of chemical fertilizers has increased every year, and with it, the utilization and efficiency of chemical fertilizers have become lower and lower. The efficiency of crop yields has decreased year by year and the use of chemical fertilizers has increased year by year. Soil fertility has also decreased, and soil desertification has become more and more. Serious; the use of pesticides is also increasing year by year, causing environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/11
CPCC12N1/205C12R2001/11
Inventor 钟增涛曹亚君江高飞马东燕杨善忠韩福忠
Owner NANJING AGRICULTURAL UNIVERSITY
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