A kind of janus particle with chemotherapeutic/photothermal synergistic effect and preparation method thereof

Abstract

A Janus particle with chemotherapy / photothermal synergy and its preparation method, belonging to the field of biomedicine technology, can solve the technical problem that other materials are not easy to achieve multiple methods of combined treatment of cancer. The particle is gold-silica / mesoporous Silica Janus nanostructure, gold nanoparticles are in situ reduced on the surface of aminated mesoporous silica hemisphere by Pickering emulsion method, and different drugs are loaded on both ends. Aminated mesoporous silica Janus particles modified by gold nanoparticles are prepared by the Pickering emulsion method. The Janus particles of the present invention can simultaneously load two kinds of drugs, and can precisely control the ratio of the two kinds of drugs. The two kinds of drugs can pass pH, oxidation Reduction and other external signal stimuli respond to drug release, which solves the technical problem of drug burst release caused by existing drug nanocarriers.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a Janus particle with chemotherapy / photothermal synergistic effect and a preparation method thereof. Background technique

[0002] Chemotherapy is the main treatment for most cancers, but the current traditional chemotherapy drugs still have the problems of poor specificity and severe side effects. In recent years, drug delivery systems based on nanomaterials have attracted much attention due to their good efficacy and low toxicity. However, in order to further improve the treatment efficiency in actual treatment, multi-drug synergistic treatment is usually required. However, it is difficult for isotropic particles to simultaneously load and release drugs with different physical and chemical properties, and it is not easy to synergize with multiple diagnosis and treatment methods.

[0003] The surfaces of Janus particles have different morphology or chemical fun...

Examples

Embodiment 1

[0028] The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, centrifuge, remove the supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution at 80°C Reflux for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-aminopropanol Triethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, and the bottom precipitate was washed and dried in vacuum to obtain aminated mesoporous silica nanoparticles;

[0029] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: disperse 20 mg of ...

Embodiment 2

[0033]The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, centrifuge, remove the supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution at 80°C Reflux for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-aminopropanol Triethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, and the bottom precipitate was washed and dried in vacuum to obtain aminated mesoporous silica nanoparticles;

[0034] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: disperse 50 mg of a...

Embodiment 3

[0038] The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, then centrifuge, remove the last supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution for 80 Reflux at ℃ for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-ammonia Propyltriethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, the bottom precipitate was washed, and then vacuum-dried to obtain aminated mesoporous silica nanoparticles;

[0039] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: 50 mg...

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a Janus particle with chemotherapy / photothermal synergistic effect and a preparation method thereof. Background technique

[0002] Chemotherapy is the main treatment for most cancers, but the current traditional chemotherapy drugs still have the problems of poor specificity and severe side effects. In recent years, drug delivery systems based on nanomaterials have attracted much attention due to their good efficacy and low toxicity. However, in order to further improve the treatment efficiency in actual treatment, multi-drug synergistic treatment is usually required. However, it is difficult for isotropic particles to simultaneously load and release drugs with different physical and chemical properties, and it is not easy to synergize with multiple diagnosis and treatment methods.

[0003] The surfaces of Janus particles have different morphology or chemical fun...

Examples

Embodiment 1

[0028] The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, centrifuge, remove the supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution at 80°C Reflux for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-aminopropanol Triethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, and the bottom precipitate was washed and dried in vacuum to obtain aminated mesoporous silica nanoparticles;

[0029] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: disperse 20 mg of ...

Embodiment 2

[0033]The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, centrifuge, remove the supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution at 80°C Reflux for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-aminopropanol Triethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, and the bottom precipitate was washed and dried in vacuum to obtain aminated mesoporous silica nanoparticles;

[0034] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: disperse 50 mg of a...

Embodiment 3

[0038] The first step is to prepare aminated mesoporous silica nanoparticles: add 0.25 g cetyltrimethylammonium bromide (CTAB) to 120 mL water, raise the temperature to 80 °C, add 0.875 mL 2 mol / L hydroxide Sodium solution, add 1.25 mL of tetraethyl orthosilicate (TEOS) to the above solution, stir at 80°C for 2 h, then centrifuge, remove the last supernatant, and redisperse the bottom precipitate in 6.0% hydrochloric acid-methanol solution for 80 Reflux at ℃ for 8 hours to remove CTAB, and vacuum-dry the bottom precipitate after centrifugation to obtain dry mesoporous silica nanoparticles. Disperse 1.0 g of dry mesoporous silica nanoparticles in 50 mL of methanol, add 1.0 mL of 3-ammonia Propyltriethoxysilane (APTES) was refluxed at 80°C for 8 h, centrifuged, the bottom precipitate was washed, and then vacuum-dried to obtain aminated mesoporous silica nanoparticles;

[0039] The second step is to prepare the Pickering emulsion of aminated mesoporous silica nanoparticles: 50 mg...

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