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Method for extracting collagen peptides from fish scales

A collagen polypeptide and fish scale collagen technology, applied in the field of bioengineering, can solve the problems of complex preparation process and low yield, and achieve the effects of high extraction efficiency, good biological activity and low cost

Inactive Publication Date: 2018-10-26
兰溪市沉默生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that the preparation process is complicated and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for extracting collagen polypeptides from fish scales comprises pretreatment of fish scales, extraction of fish scale collagens, preparation of fermentation broth, and preparation of fish scale collagen polypeptides. The specific steps are as follows:

[0024] 1) Fish scale pretreatment: wash the sticky matter and other impurities on the surface of the fish scales, dry them, and then soak the fish scales in a 3% hydrochloric acid solution for 20 hours to remove the calcium on the fish scales, and then put the decalcified Soak the fish scales in saturated sodium bicarbonate solution for 12 hours to remove the fat and impurities in the fish scales, and finally dry the treated fish scales at 40°C for later use. Pretreatment of the fish scales can remove the fat wrapped around the collagen fibers And globulin, keratin and other non-collagenous proteins and calcium in fish scales are removed, which is convenient for the subsequent extraction and separation of colla...

Embodiment 2

[0033] The method for extracting collagen polypeptides from fish scales comprises pretreatment of fish scales, extraction of fish scale collagens, preparation of fermentation broth, and preparation of fish scale collagen polypeptides. The specific steps are as follows:

[0034] 1) Fish scale pretreatment: wash the sticky matter and other impurities on the surface of the fish scales, dry them, and then soak the fish scales in a 5% hydrochloric acid solution for 24 hours to remove the calcium on the fish scales, and then put the decalcified Soak the fish scales in a saturated sodium bicarbonate solution for 16 hours to remove fat and impurities in the fish scales, and finally dry the treated fish scales at 40°C for later use;

[0035] 2) Extraction of fish scale collagen: Swell the pretreated fish scale and acidic soaking agent according to the weight ratio of 1:6 for 50 minutes, wash it repeatedly with water until neutral, drain the water and crush it, then add fish scale to the...

Embodiment 3

[0042] The method for extracting collagen polypeptides from fish scales comprises pretreatment of fish scales, extraction of fish scale collagens, preparation of fermentation broth, and preparation of fish scale collagen polypeptides. The specific steps are as follows:

[0043] 1) Fish scale pretreatment: wash the sticky matter and other impurities on the surface of the fish scales, dry them, then soak the fish scales in a 4% hydrochloric acid solution for 20 hours, and then put the decalcified fish scales into saturated sodium bicarbonate Soak in the solution for 16 hours, and finally dry the treated fish scales at 40°C for later use;

[0044] 2) Extraction of fish scale collagen: Swell the pretreated fish scale and acid soaking agent according to the weight ratio of 1:5 for 50 minutes, wash it repeatedly with water until neutral, drain the water and crush it, then add fish scale to the fish scale powder Distilled water 10 times the weight of the powder, extracted in a water ...

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PUM

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Abstract

The invention discloses a method for extracting collagen peptides from fish scales: first, cleaned fish scales are soaked in a hydrochloric acid solution and a saturated bicarbonate solution, and dried for use after soaking, then the pre-treated fish scales are swollen with an acidic soaking agent and washed with water until neutral, and crushed after draining, and then distilled water is added for hot water extraction, after which vacuum filtration is performed to remove impurities, and fish scale collagen powder is obtained after freeze drying, and finally the activated and cultured lactic acid bacteria are inoculated into the fermentation broth containing fish scale collagen powder for cultivation and fermentation, after which the filtration and centrifugation are carried out, and the supernatant is evaporated and concentrated to obtain the scale collagen polypeptide. The preparation method has the advantage of being simple and easy in operation, mild in reaction conditions, high inextraction efficiency, low in cost, and convenient in commercialization. The scale collagen polypeptide prepared has the advantage of high yield and purity, small molecular weight, good biological activity, and strong hydrophilicity. The scale collagen polypeptide is capable of being widely used in cosmetics, health food and pharmaceutical products and other fields.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for extracting collagen polypeptide from fish scales. Background technique [0002] Collagen is a natural biopolymer synthesized by animal cells. As an important component of the extracellular matrix, it exists in almost all tissues, accounting for about 30% of the total animal protein. Collagen has a high ability to resist extension, making skin and other connective tissue Tissues can maintain a certain structure and have special mechanical properties to support and protect cells, tissues and body functions, which is critical to the structure and performance of cells and tissues. [0003] Fish collagen peptide is a functional food or health product raw material. Many studies have shown that there are a large number of biologically active components in fish collagen peptide, including anti-oxidation, inhibiting ACE activity, anti-ulcer, inhibiting arthritis, impro...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P21/00C07K14/78C12R1/225C12R1/46
CPCC12P39/00C07K14/78C12P21/00
Inventor 周建波
Owner 兰溪市沉默生物科技有限公司