Preservation method of lactic acid bacteria strain
A technology for preservation of lactic acid bacteria and strains, applied in the direction of bacteria, preservation of microorganisms, and methods based on microorganisms, etc., can solve the problems of inability to preserve for a long time again, and achieve the effects of simple and easy preservation methods, simple recovery, and simple use methods
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Embodiment 1
[0020] Preservation medium was prepared, and the composition of Lactobacillus preservation medium was 10 g of peptone, 8 g of beef extract powder, 2 g of yeast extract powder, 20 g of glucose, 2 g of dipotassium hydrogen phosphate, 2 g of diammonium hydrogen citrate, and sodium acetate. 5 g, 0.2 g magnesium sulfate, 0.04 g manganese sulfate, 20 g calcium carbonate, 2 g Tween-80, and 1000 g water, pH 7.0, sterilized by pressurized steam at 121°C for 15 minutes, and set aside.
[0021] Lactobacillus delbrueckii subsp. bulgaricus ( Lactobacillus delbrueckii subsp. bulgaricus ) culture preparation, insert Lactobacillus delbrueckii subsp. bulgaricus into Lactobacillus preservation medium, when the pH value of the culture solution is lower than 5.0, adjust the pH to 6.5 with sodium hydroxide solution, and cultivate at 37°C for 24 h , to terminate the cultivation. Centrifuge the culture liquid of Lactobacillus delbrueckii subsp. bulgaricus to collect the bacterial cells. Set the t...
Embodiment 2
[0023] Preservation medium was prepared, and the composition of lactococcus preservation medium was 5 g of soybean peptone, 5 g of peptone, 2.5 g of casein peptone, 3 g of yeast extract powder, 3 g of beef extract powder, 10 g of lactose, 0.5 g of sodium ascorbate, and 0.5 g of glycerol phosphate Sodium 20g, magnesium sulfate 0.3g, calcium carbonate 20g and water 1000g, pH value 7.0, sterilized by pressurized steam at 121°C for 15 minutes, set aside.
[0024] Streptococcus thermophilus culture ( Streptococcus thermophilus) preparation, the Streptococcus thermophilus species were inserted into the Lactococcus preservation medium, when the pH value of the culture solution was lower than 5.0, the pH was adjusted to 6.5 with sodium hydroxide solution, and the culture was terminated after 20 h at 42°C. Centrifuge the culture liquid of Streptococcus thermophilus to collect the bacteria, set the temperature of the centrifuge to 4°C, the centrifugation speed to 6000 r / min, and the ce...
Embodiment 3
[0026] Preservation medium was prepared, and the composition of Lactobacillus preservation medium was 5 g of peptone, 3 g of beef extract powder, 6 g of yeast extract powder, 5 g of glucose, 1 g of dipotassium hydrogen phosphate, 1 g of diammonium hydrogen citrate, and 3 g of sodium acetate. g, 0.1 g magnesium sulfate, 0.02 g manganese sulfate, 10 g calcium carbonate, 1 g Tween-80 and 1000 g water, pH 6.0, sterilized by pressurized steam at 118°C for 15 minutes, and set aside. Lactobacillus acidophilus ( Lactobacillus acidophilus ) culture preparation, the Lactobacillus acidophilus strains were inserted into the Lactobacillus preservation medium, cultured at 30°C for 36 h, and then the culture was terminated. Centrifuge the culture of Lactobacillus acidophilus to collect the bacteria, set the temperature of the centrifuge to 0°C, the centrifugal speed to 9000 r / min, and the centrifugation time to 10 minutes. After the centrifugation, remove the supernatant and collect the pre...
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