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Method for obtaining eremochloa ophiuroide mutant

A technology for the cultivation of Pseudomonas spp. and mutants is applied in the field of obtaining Pseudomonas spp. mutants, which can solve the problems of somatic cell mutation breeding of plants treated with low-temperature plasma, and achieve the advantages of shortening the breeding cycle, low cost, and speeding up the breeding process. Effect

Active Publication Date: 2018-11-23
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

However, so far, there has been no research report on the somatic mutation breeding of plants treated with low-temperature plasma, nor has there been any research report on the mutagenesis breeding of turfgrass (including Pseudomonas spp.

Method used

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  • Method for obtaining eremochloa ophiuroide mutant
  • Method for obtaining eremochloa ophiuroide mutant
  • Method for obtaining eremochloa ophiuroide mutant

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preparation example Construction

[0034] The method for obtaining the mutant of C. chinensis of the present invention comprises taking the callus of C. sylvestris and performing low-temperature plasma mutagenesis to obtain the mutagenized callus. The present invention has no special limitation on the type and source of the sage grass, and the conventional sage grass in the field can be used. The method for preparing the callus of P. chinensis according to the present invention preferably comprises: taking the sterilized young ears of P. spp. ; The induction medium uses MS medium as the basic medium, and also includes 1-5 mg / L of 2,4-dichlorophenoxyacetic acid, 0.04-0.2 mg / L of 6-benzylaminopurine, and 20-40 g / L of sucrose And plant gel (fixed support callus) 1 ~ 5g / L. The young ears of C. chinensis in the present invention are preferably the young ears of C. chinensis after the leaf sheaths have been removed. The method of the removal is not particularly limited in the present invention, and conventional meth...

Embodiment 1

[0049] Take the fresh young ears of Pseudomonas chinensis strain 'Common', peel off the leaf sheaths, disinfect and sterilize with 75% alcohol, and then inoculate them into the induction medium (the medium formula is: MS medium, add 2,4-dichlorophenoxy Acetic acid 3mg / L and 6-benzylaminopurine 0.1mg / L, additional sucrose 30g / L, phytogel 3g / L, pH 5.8), cultivated in dark at 25±1°C for 3 weeks, to obtain light yellow, granular embryogenic callus.

[0050] Put 20 embryogenic calli with a diameter of about 0.5 cm in a glass petri dish, seal it with a parafilm, place it in a low-temperature plasma processor, and accept low-temperature plasma treatment generated by glow discharge. The processing parameters are as follows: the processing power is 300W, the processing time is 90s, and the medium is a mixed gas of helium and air (He:air=8:1).

[0051] The embryogenic callus after the low-temperature plasma treatment was inoculated into the differentiation medium (the formula of the me...

Embodiment 2

[0056] Take the fresh young ears of Tifblair variety 'Tifblair', peel off the leaf sheaths, disinfect and sterilize with 75% alcohol, and then inoculate them into the induction medium (the formula of the medium is: MS medium, add 2,4-dichlorophenoxy Acetic acid 3mg / L and 6-benzylaminopurine 0.1mg / L, additional sucrose 30g / L, phytogel 3g / L, pH 5.8), cultivated in dark at 25±1°C for 3 weeks, to obtain light yellow, granular embryogenic callus.

[0057] Put 20 embryogenic calli with a diameter of about 0.5 cm in a glass petri dish, seal it with a parafilm, place it in a low-temperature plasma processor, and accept low-temperature plasma treatment generated by glow discharge. The processing parameters are as follows: the processing power is 400W, the processing time is 90s, and the medium is a mixed gas of helium and air (He:air=8:1).

[0058]The embryogenic callus after the low-temperature plasma treatment was inoculated into the differentiation medium (the formula of the medium...

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Abstract

The invention provides a method for obtaining an eremochloa ophiuroide mutant, and belongs to the technical field of turfgrass biology. The method comprises the following steps of (1) obtaining mutated callus tissues; (2) performing differentiation culture on the mutated callus tissues to obtain induction seedlings; (3) inoculating the induction seedlings for performing rooting culture to obtain complete plants; (4) screening the complete plants to obtain variant plants; (5) performing cutting breeding on the variant plants; authenticating the mutant character; obtaining a stable and genetic eremochloa ophiuroide mutant. When the method is used, through once mutagenesis, 23 to 35 complete plants can be obtained; 5 to 10 complete plants generate variation on one or various characters; the character mutation rate is 21.73 to 28.57 percent; 2 to 3 plants generate DNA variation; the variation rate is 5.7 to 8.7 percent.

Description

technical field [0001] The invention belongs to the field of turfgrass biotechnology, and in particular relates to a method for obtaining a mutant of turfgrass. Background technique [0002] Eremochloa ophiuroides (Munro.) Hack.) is an excellent warm-season turfgrass and forage grass originated in my country. It has the advantages of beautiful leaf shape, long green period, resistance to barrenness, less pests and diseases, and low maintenance level. Known as "the best warm-season lawn grass in China", it is widely used in the establishment of garden lawns, rest lawns and slope protection lawns, especially suitable for water and soil conservation and large-scale landscape construction. With the depletion of global water resources and energy and the increasing importance of ecological civilization construction, the demand for chrysanthemum grasses, especially those with good quality and excellent traits, is increasing. However, there is a single variety of the existing false ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06
CPCA01H1/06A01H4/001A01H4/008
Inventor 李玲刘建秀宗俊勤李建建汪毅郭海林陈静波李丹丹郭爱桂
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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