Preparation method of cellulose porous medical stent dressing enhanced by nanometer silk protein powder

A nano-cellulose and nano-silk fibroin technology, which is applied in the field of medical textile materials, can solve the problems of limited concentration, difficulty in meeting the requirements for the use of large-area medical composite materials, and limited solid content and size of medical composite materials, and achieves improved crystallization. degree, excellent biocompatibility, and the effect of improving mechanical properties

Inactive Publication Date: 2018-11-23
DONGGUAN LIANZHOU INTPROP OPERATION MANAGEMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen from the above prior art that the combination of cellulose material and silk protein can be used to prepare medical composite materials with excellent performance, but at present, cellulose and silk protein are mostly mixed in the form of solution, and the concentration is limited, and then the medical composite material prepared Solid content or size is limited, it is difficult to meet the use requirements of large-area medical composite materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) The cellulose cotton pulp is vibrated by an ultrasonic cell pulverizer to obtain a nanocellulose slurry, which is transferred to a sheet abrasive tool and freeze-dried to obtain a sheet-shaped cellulose porous scaffold layer, wherein the sheet-shaped cellulose porous scaffold The thickness of the layer is 1 μm, the pore size is 30 μm, and the porosity is not less than 20%.

[0026] (2) Add silkworm cocoons to sodium carbonate aqueous solution, boil and degumming for 30 minutes, repeat twice, after drying, dissolve in lithium bromide aqueous solution at 60°C, dialyze and centrifuge to obtain silk fibroin aqueous solution, according to silk fibroin and neutral protease The mass ratio of silk fibroin is 10:1, add neutral protease to the silk fibroin aqueous solution, adjust the pH value to 6, stir and hydrolyze at 35°C, and vacuum freeze-dry to obtain nano-scale silk fibroin powder with a particle size of 300-1000nm .

[0027] (3) Spray nano-scale silk fibroin powder ...

Embodiment 2

[0030] (1) The cellulose cotton pulp is vibrated by an ultrasonic cell pulverizer to obtain a nanocellulose slurry, which is transferred to a sheet abrasive tool and freeze-dried to obtain a sheet-shaped cellulose porous scaffold layer, wherein the sheet-shaped cellulose porous scaffold The thickness of the layer is 3 μm, the pore size is 80 μm, and the porosity is not less than 20%.

[0031](2) Add silkworm cocoons to sodium carbonate aqueous solution, boil and degumming for 30 minutes, repeat 3 times, after drying, dissolve in lithium bromide aqueous solution at 60°C, dialyze and centrifuge to obtain silk fibroin aqueous solution, according to silk fibroin and neutral protease The mass ratio of silk fibroin is 15:1, add neutral protease to the silk fibroin aqueous solution, adjust the pH value to 7, stir and hydrolyze at 45°C, and vacuum freeze-dry to obtain nano-scale silk fibroin powder with a particle size of 300-1000nm .

[0032] (3) Spray nano-scale silk fibroin powder...

Embodiment 3

[0035] (1) The cellulose cotton pulp is vibrated by an ultrasonic cell pulverizer to obtain a nanocellulose slurry, which is transferred to a sheet abrasive tool and freeze-dried to obtain a sheet-shaped cellulose porous scaffold layer, wherein the sheet-shaped cellulose porous scaffold The thickness of the layer is 2 μm, the pore size is 50 μm, and the porosity is not less than 20%.

[0036] (2) Add silkworm cocoons to sodium carbonate aqueous solution, boil and degumming for 30 minutes, repeat twice, after drying, dissolve in lithium bromide aqueous solution at 60°C, dialyze and centrifuge to obtain silk fibroin aqueous solution, according to silk fibroin and neutral protease The mass ratio of silk fibroin is 13:1, add neutral protease to the silk fibroin aqueous solution, adjust the pH value to 6.5, stir and hydrolyze at 40°C, and vacuum freeze-dry to obtain nano-scale silk fibroin powder with a particle size of 300-1000nm .

[0037] (3) Spray nano-scale silk fibroin powde...

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Abstract

The invention provides a preparation method of a cellulose porous medical stent dressing enhanced by nanometer silk protein powder. The preparation method comprises the following steps of oscillatinga cellulose cotton slurry by an ultrasonic cell crushing machine, so as to obtain a nanometer cellulose slurry; transferring the slurry into a flaky grinding device, and performing freeze-drying, so as to obtain a flaky cellulose porous stent layer; adding neutral protease into an aqueous solution of silk fibroin to hydrolyze, and performing freeze-drying under the vacuum condition, so as to obtain a nanometer silk fibroin powder; coating a position between the flaky porous layered materials with the nanometer silk fibroin powder in a sandwich way, and oscillating to disperse the nanometer silk fibroin powder into the flaky porous layered material, so as to obtain a nanometer silk fibroin powder / cellulose porous stent blank; removing the packaging material of the porous stent blank, soaking into a deionized aqueous solution, fully swelling, then heating, pressurizing, curing, and drying, so as to obtain the cellulose porous medical stent dressing enhanced by the nanometer silk proteinpowder.

Description

technical field [0001] The invention belongs to the technical field of medical textile materials, and in particular relates to a preparation method of a cellulose porous medical support dressing reinforced by nano silk protein powder. Background technique [0002] Biomedical materials refer to new high-tech materials that can be used in organisms for diagnosis, treatment, repair or replacement of their diseased tissues and organs, and can act on living systems or enhance their functions. Biomaterials should have good biocompatibility, and it should not cause inflammation and immune rejection. Due to its good biocompatibility, biodegradability and low inflammatory response in the body, silk fibroin can be slowly degraded into amino acids and polypeptides in the body, and gradually metabolized and absorbed by the body. It has a wide range of applications in the field of biomedical materials prospect. Cellulose is a linear polymer composed of cellobiose repeating units bonded...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/56A61L27/48A61L15/42A61L15/28A61L15/32D06M15/15D06M101/06
CPCA61L27/50A61L15/28A61L15/32A61L15/42A61L15/425A61L27/48A61L27/56D06M15/15D06M2101/06C08L1/02C08L89/00
Inventor 徐亚维
Owner DONGGUAN LIANZHOU INTPROP OPERATION MANAGEMENT CO LTD
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