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Use of Isochrysis polypeptide IZP-2 in preparation of drugs and health products for preventing and treating alcoholic liver injury

A technology of alcoholic liver injury and IZP-2, applied in the field of peptides

Active Publication Date: 2018-11-27
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]At present, there is no research on the protective effect and mechanism of Isochrysis zhanjiang polypeptide on alcohol-induced cell damage

Method used

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  • Use of Isochrysis polypeptide IZP-2 in preparation of drugs and health products for preventing and treating alcoholic liver injury
  • Use of Isochrysis polypeptide IZP-2 in preparation of drugs and health products for preventing and treating alcoholic liver injury
  • Use of Isochrysis polypeptide IZP-2 in preparation of drugs and health products for preventing and treating alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the mensuration of DPPH free radical scavenging capacity

[0035] Experimental principle: DPPH (1,1-diphenyl-2-picrylhydrazyl), that is, 1,1-diphenyl-2-trinitrophenylhydrazine free radical. There are π bonds of the benzene ring and multiple -NO in the molecule 2 , so that nitrogen free radicals can exist stably. Its organic alcohol solution is purple, with maximum light absorption at 517nm wavelength. When the free radical scavenger provides an electron to pair with the lone pair of DPPH free radicals, the purple itself turns yellow, and DPPH is removed. At this time, the absorbance A value at the wavelength of 517nm becomes smaller, and the degree of change is linearly related to the degree of free radical scavenging, that is, the stronger the scavenging ability of the free radical scavenger, the smaller the absorbance. The detection of provides a simple and ideal model.

[0036]Experimental process: Weigh 0.1972g of DPPH reagent, dissolve it with 95%...

Embodiment 2

[0041] Example 2: Effect of polypeptide IZP-2 on the relative viability of HepG2 cells

[0042] 2.1 Determination of the conditions of the alcohol-damaged cell model and the determination of the peptide concentration (MTT method)

[0043] Experimental principle: The MTT method is based on the living cell metabolite reducing agent MTT {3-(4,5)-dimethylthiahiazo(-z-yl)-3,5-di-phenyltetrazoliumromide}thiazolium blue. It is a yellow compound dye that accepts hydrogen ions. It can act on the respiratory chain in the mitochondria of living cells. Under the action of succinate dehydrogenase, it produces blue-purple formazan (formazan) crystals. The amount of formazan crystals produced It is only proportional to the number of living cells (because succinate dehydrogenase disappears in dead cells and has no reducing function). Formazan crystals produced by reduction can be dissolved with dimethyl sulfoxide solution (DMSO). The color of the solution is proportional to its content. Use ...

Embodiment 3

[0060] Embodiment 3: Agarose gel electrophoresis

[0061] Experimental principle: Agarose gel electrophoresis is widely used in nucleic acid analysis and is an indispensable and important analysis method in nucleic acid research. Nucleic acid molecules are amphoteric dissociation molecules. In the electrophoresis buffer higher than its isoelectric point, the phosphate groups are all dissociated, but the bases are not dissociated. Therefore, the nucleic acid molecules are negatively charged and migrate to the positive pole during electrophoresis. Agarose is a polymer chain linear molecule, which is mainly extracted from marine plant agar and modified by glycosylation. Using agarose gel as the electrophoresis support medium, it functions as a molecular sieve, making nucleic acid molecules with different conformations and sizes There is a large difference in mobility to achieve the purpose of separation.

[0062] 3.1 DNA extraction

[0063] Take HepG2 cells in the logarithmic g...

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PUM

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Abstract

The invention relates to use of isochrysis polypeptide IZP-2 in the preparation of drugs, health products or diet replenishers for preventing and treating alcoholic liver injury. The amino acid sequence of the Isochrysis polypeptide IZP-2 is Phe-Glu-Ile-His-Cys-Cys-Gly. A polypeptide IZP-2 derived from Isochrysis is capable of eliminating free radicals, inhibiting the cellular damage induced by alcohol and the breakage of DNA in the oxidation reaction and reducing the content of ROS in cells and has no toxicity to cells.

Description

technical field [0001] The invention relates to the field of polypeptides, in particular to the use of Isochrysis polypeptide IZP-2 in the preparation of medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Background technique [0002] Zhanjiang Isochrysis (Isochrysis zhangjiangensis Hu 1992), is a widely distributed microscopic marine planktonic unicellular algae, belonging to Chrysophyta, Chrysophyceae, Isochrysidales, Isochrysidaceae. Because of its small size, rapid reproduction, rich nutrition, fat content of 26.35%, and no cellulose in the cell wall, it is easy to be caught, swallowed, digested and absorbed by shellfish larvae. It is a good basic bait for bivalve larvae in the marine economy. However, at present, the development and research on the biological activity of Isoflagellates Zhanjiang are less. [0003] Alcoholic liver disease (ALD) is a liver damage disease caused by long-term heavy drinking. Studies have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P1/16A23L33/18
CPCA61K38/08A61P1/16A23L33/18A23V2002/00A23V2200/334A23V2250/55
Inventor 千忠吉梁鹏李承勇周春霞洪鹏志孙省利
Owner GUANGDONG OCEAN UNIVERSITY
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