Three-dimensional mineralized collagen scaffold material and bone regeneration application thereof

A technology of mineralized collagen and scaffold materials, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of increased incidence of cancer and high cost, and promote new bone regeneration. The preparation process is simple and easy, and it is convenient for growth and differentiation effects

Inactive Publication Date: 2018-12-04
北京恒泽博泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Traditionally, biomaterials combined with osteoblasts and osteogenic proteins are usually used as a powerful method to repair bone defects. The disadvantages of this method are high cost and side effects caused by exogenous cells and growth factors. Such as respiratory effects, heterotopic bone formation, in situ bone resorption at the transplant site, and possible increased incidence of cancer, etc.

Method used

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  • Three-dimensional mineralized collagen scaffold material and bone regeneration application thereof
  • Three-dimensional mineralized collagen scaffold material and bone regeneration application thereof
  • Three-dimensional mineralized collagen scaffold material and bone regeneration application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043](1) Extraction of type I collagen: Weigh 1 kg of bovine tendon and chop it with a meat grinder and wash it, add a large amount of acetone to soak for 1 day to remove the surface fat tissue, dry it at room temperature, add 2L of 0.5M acetic acid solution at 4 Stir at ℃ for 1 day, add 100 g of pepsin to the system and continue to stir for 2 days, and continuously add new acetic acid solution until the total volume is 5 L. The mixture is coarsely filtered with gauze, and the obtained filtrate is collagen crude extract. NaCl was added to the mixture to a concentration of 2M for salting out, and the precipitate was separated by low-temperature centrifugation and then dissolved in 0.5M acetic acid solution. , Dialyzed in deionized water, and finally freeze-dried to obtain high-purity type I collagen;

[0044] (2) Preparation of nano-hydroxyapatite: the CaCl of 66mg 2 2H 2 The polyacrylic acid (MW=2000) of O and 25mg is dissolved in the TBS damping fluid of 50mL and obtains A...

Embodiment 2

[0049] (1) Extraction of type I collagen: Weigh 1 kg of bovine tendon and chop it with a meat grinder and wash it, add a large amount of acetone to soak for 1 day to remove the surface fat tissue, dry it at room temperature, add 2L of 0.5M acetic acid solution at 4 Stir at ℃ for 1 day, add 100 g of pepsin to the system and continue to stir for 2 days, and continuously add new acetic acid solution until the total volume is 5 L. The mixture is coarsely filtered with gauze, and the obtained filtrate is collagen crude extract. NaCl was added to the mixture to a concentration of 2M for salting out, and the precipitate was separated by low-temperature centrifugation and then dissolved in 0.5M acetic acid solution. , Dialyzed in deionized water, and finally freeze-dried to obtain high-purity type I collagen;

[0050] (2) Preparation of nano-hydroxyapatite: the CaCl of 66mg 2 2H 2 The polyacrylic acid (MW=2000) of O and 25mg is dissolved in the TBS damping fluid of 50mL and obtains ...

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Abstract

The invention discloses a three-dimensional mineralized collagen scaffold material and a bone regeneration application thereof. A method for preparing the three-dimensional mineralized collagen scaffold material having a strict grade structure by binary assembling comprises the following steps: preparing nano-hydroxyapatite; performing dialysis in the collagen mineralization process to make the nano-hydroxyapatite slowly enter and be deposited on the surface of collagen fibers; and controlling various parameters in vitro to complete the simulation of natural bone mineralized collagen fibers. The three-dimensional mineralized collagen scaffold prepared by completely duplicating chemical components and periodic grade structure of natural bones has a proper mechanical strength, and can recruit host cells to realize osteogenic differentiation. The three-dimensional mineralized collagen scaffold material having the strict grade structure has excellent angiogenesis ability, and can be individually used to realize bone defect repair in in vivo animal test without adding any biological preparation, such osteogenic factors or recombinant proteins.

Description

technical field [0001] The invention relates to the field of preparation and treatment of bone tissue repair materials, in particular to a three-dimensional mineralized collagen scaffold material and its bone regeneration application. Background technique [0002] Traditionally, biomaterials combined with osteoblasts and osteogenic proteins are usually used as a powerful means to repair bone defects. The disadvantages of this method are high cost and side effects caused by exogenous cells and growth factors. Such as respiratory effects, heterotopic bone formation, in situ bone resorption at the graft site, and possible increased incidence of cancer. Therefore, the development of osteoinductive biomaterials that utilize the body's autonomous regeneration ability to induce bone formation by recruiting host cells is still a clinical problem that needs to be solved urgently. The key to this technology is to develop a target-specific bioscaffold material to mimic the hierarchica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/24A61L27/12A61L27/56
CPCA61L27/12A61L27/24A61L27/50A61L27/56A61L2400/12A61L2430/02
Inventor 刘燕周彦恒罗聃
Owner 北京恒泽博泰生物科技有限公司
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