Preparation method of feed containing chitin-oligosaccharides

A technology for chitosan oligosaccharide and feed, which is applied in the field of preparing feed containing chitosan oligosaccharide, can solve the problems of dense chitin structure, unfavorable biological decomposition, high energy consumption, etc., and achieves the effects of saving water consumption and reducing production costs.

Inactive Publication Date: 2018-12-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation of chitooligosaccharides by fermentation still has problems such as the compact structure of chitin, which is not conducive to biodegradation, and the energy consumption in the liquid fermentation process is too large. It is necessary to provide a more effective method.

Method used

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  • Preparation method of feed containing chitin-oligosaccharides
  • Preparation method of feed containing chitin-oligosaccharides
  • Preparation method of feed containing chitin-oligosaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Thaw 100g of frozen prawn heads at room temperature, add 500mL of deionized water, homogenize with a high-speed homogenizer, collect the homogenate of shrimp heads, put it into a steel reaction vessel, and pass in CO 2 Until the container pressure reaches 1.0MPa, the oil bath heating temperature is 200°C, and the heating time is 60min. After heat treatment, concentrate at 60°C and 20mBar until the moisture content of the shrimp head raw material is 30%, add 100g of bran, and sterilize the shrimp head and bran mixture at 121°C for 20 minutes before use.

[0022] Get Bacillus subtilis bacterial classification, inoculate in seed culture medium (25g / L glucose, 25g / L yeast powder, 1g / LKH 2 PO 4 , 0.5g / L NaCl, 0.5g / L MgSO 4 ·7H 2 (0) in a shaker at 37° C. and 200 rpm for 24 hours to obtain a seed culture solution. Inoculate into the sterilized shrimp head bran mixture according to 10% (v / w) inoculum amount, and add appropriate amount of sterile water to adjust the water c...

Embodiment 2

[0025] Thaw 100g of frozen prawn heads at room temperature, add 500mL of deionized water, homogenize with a high-speed homogenizer, collect the homogenate of shrimp heads, put it into a steel reaction vessel, and pass in CO 2 Until the container pressure reaches 1.0MPa, the oil bath heating temperature is 200°C, and the heating time is 60min. After heat treatment, concentrate at 60°C and 20mBar until the moisture content of the shrimp head raw material is 30%, add 100g of bran, and sterilize the shrimp head and bran mixture at 121°C for 20 minutes before use.

[0026] Get Bacillus subtilis bacterial classification, inoculate in seed culture medium (25g / L glucose, 25g / L yeast powder, 1g / LKH 2 PO 4 , 0.5g / L NaCl, 0.5g / L MgSO 4 ·7H 2 (0) in a shaker at 37° C. and 200 rpm for 24 hours to obtain a seed culture solution. Inoculate into the sterilized shrimp head bran mixture according to 10% (v / w) inoculum amount, and add appropriate amount of sterile water to adjust the water c...

Embodiment 3

[0029] Thaw 100g of frozen prawn heads at room temperature, add 500mL of deionized water, homogenize with a high-speed homogenizer, collect the homogenate of shrimp heads, put it into a steel reaction vessel, and pass in CO 2 Until the container pressure reaches 1.5MPa, the oil bath heating temperature is 180°C, and the heating time is 60min. After heat treatment, concentrate at 60°C and 20mBar until the moisture content of the shrimp head raw material is 30%, add 100g of bran, and sterilize the shrimp head and bran mixture at 121°C for 20 minutes before use.

[0030] Get Bacillus licheniformis bacterial classification, inoculate in seed culture medium (25g / L glucose, 25g / L yeast powder, 1g / LKH 2 PO 4 , 0.5g / L NaCl, 0.5g / L MgSO 4 ·7H 2 (0) in a shaker at 37° C. and 200 rpm for 24 hours to obtain a seed culture solution. Inoculate into the sterilized shrimp head bran mixture according to 10% (v / w) inoculum amount, and add appropriate amount of sterile water to adjust the wa...

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Abstract

The present invention discloses a preparation method of a feed containing chitin-oligosaccharides. The preparation method comprises the following steps: firstly, drying and crushing shrimp heads, adding deionized water, conducting uniform mixing to obtain homogenate, and placing the homogenate in a steel reactor; introducing CO2 into the steel reactor, conducting sealing, and placing the steel reactor in an oil bath for heating; taking out the heated shrimp head homogenate, and putting the homogenate into a vacuum concentration instrument for concentration; sterilizing the concentrated shrimphead raw material, and inoculating the raw material with microorganisms for fermentation; and adding deionized water after fermentation, placing the obtained mixture in a stirrer for heat insulation with stirring, and drying and crushing a sample after the stirring to obtain the sample containing chitin-oligosaccharides. The method avoids the defects that a large amount of acid and alkali are usedin the process for producing chitin-oligosaccharides by traditional chemical methods so as to generate industrial wastewater which pollutes the environment. Meanwhile, compared with enzymatic methods, the method does not need to be additionally added with the chitin enzyme, and greatly reduces the production cost.

Description

technical field [0001] The invention relates to the technical field of preparing chitooligosaccharide-containing feed by utilizing microbial solid-state fermentation technology and using shrimp heads as raw materials. Background technique [0002] Chitooligosaccharides are linear oligosaccharides linked by 2 to 10 acetylglucosamines, and are usually water-soluble oligosaccharides formed by degrading chitin. Chitooligosaccharides have biological functions such as anti-oxidation, growth-promoting, improving immunity, and regulating intestinal health, and have the characteristics of being pure natural, degradable, and high in biological safety. Therefore, chitosan can be used as a new type of green feed additive, which has the functions of improving animal disease resistance, promoting growth, and improving meat quality. It has broad application prospects and can partially replace the use of traditional chemical drugs and antibiotics. Sustained and healthy development. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23K10/12A23K10/26
CPCA23K10/12A23K10/26A23K20/163
Inventor 毛相朝孙建安赵甜宇薛长湖
Owner OCEAN UNIV OF CHINA
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