A kind of method of preparing caffeoyl tartaric acid standard substance from thorn grape juice

Abstract

The invention discloses a method for separating and preparing a caftaric acid standard product in a vitis davidii juice by using high-speed countercurrent chromatography. The method comprises the following steps: enriching the vitis davidii juice by using macroporous adsorption resin, eluting the material with ethanol, performing freeze-drying to obtain a crude sample, and performing high-speed countercurrent separation on the crude sample through solvent system water. The invention establishes the method for separating a polyphenol monomer in the vitis davidii juice by the high-speed countercurrent chromatography, and the method identifies the seperated monomer as caftaric acid by the methods such as HPLC-MS and nuclear magnetic resonance. The caftaric acid has an important pharmacological function, and its standard product is expensive, and the caftaric acid prepared by the method of the present invention has a purity of 95.8% and a calculated yield of 61.99 mg / L. The method of the high-purity standard product prepared can satisfy the large-scale preparation of caftaric acid of vitis davidii.

Description

technical field [0001] The invention relates to a method for preparing a standard product of caffeoyl tartaric acid, in particular to a method for preparing a standard product of caffeoyl tartaric acid from thorn grapes. Background technique [0002] Thorn grapes ( Vitis davidii Foex) belongs to the East Asian species of Vitis genus, and has a wide range of planting areas in Shaanxi, Gansu, Central China, South China and Southwest China. Among them, Hunan is one of the provinces where thorny grapes are concentrated and has a cultivation history of more than 200 years, with the most in western and southern Hunan [Shi Xuehui, Wang Yizhi, Chen Zuyu, etc. Preliminary report on research on botanical characters and disease resistance of Hunan thorny grapes[ J]. Chinese and Foreign Grapes and Wine, 2002 (02): 22-24]. A number of studies have shown that thorny grapes have high yield, strong resistance, good fruit flavor, rich juice, high sugar-acid ratio, high content of natural ...

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Problems solved by technology

Hu Xiaodan et al. used high-speed countercurrent chromatography to separate perilla leaf anthocyanins and malonyl perilla ning monomers from the crude extract of perilla leaf anthocyanins [Hu Xiaodan, Sun Aidong, Zhang Dequan. Study on the separation of perilla leaf anthocyanins by high-speed countercurrent chromatography ( English) [J]. Chinese Materia Medica, 2010, 33 (10): 1586-1588.] At present, there are few reports on the extraction of caffeoyl tartaric acid from grape thorns, and there are also efficient preparation methods for standard products suitable for liquid chromatography detection. not yet announced

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