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Kit for determining four antifungal drugs in dry blood spot at same time as well as application thereof

A technology of antifungal drugs and kits, which is applied in the field of clinical testing, can solve the problems of no immunosuppressant detection methods in dried blood slices, and achieve reduced biosafety risks, strong detection specificity, high precision and accuracy Effect

Inactive Publication Date: 2018-12-18
易达精准(杭州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Dried blood filter paper sample collection technology has incomparable advantages over whole blood samples, including better stability at room temperature, minimal invasiveness, lower cost, easy storage and transportation, and reduced potential risk of pathogen infection. Methods for the detection of immunosuppressants

Method used

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  • Kit for determining four antifungal drugs in dry blood spot at same time as well as application thereof
  • Kit for determining four antifungal drugs in dry blood spot at same time as well as application thereof
  • Kit for determining four antifungal drugs in dry blood spot at same time as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] In the kit: the diameter of the dried blood spot of the standard sample and the quality control sample is 3mm; the extraction solution is composed of deuterated internal standard, water, and methanol, the content of each deuterated internal standard is 1ng / mL, water 10mL, methanol 90mL; diluted The reagent is composed of methanol and water, 10mL of water and 90mL of methanol; the organic phase is acetonitrile, and the water phase is 5mmol / L ammonium acetate-0.1% acetic acid.

Embodiment 2

[0086] In the kit: the diameter of the dried blood spot of the standard sample and the quality control sample is 6mm; the extract solution is composed of deuterated internal standard, water, and acetonitrile, the content of each deuterated internal standard is 20ng / mL, water 10mL, acetonitrile 90mL; diluted The reagent is composed of acetonitrile and water, water 10mL, acetonitrile 90mL; organic phase is acetonitrile, water phase is 2mmol / L ammonium formate-0.5% acetic acid.

Embodiment 3

[0088] In the kit: the diameter of the dried blood spot of the standard sample and the quality control sample is 6mm; the extraction solution is composed of deuterated internal standard, water, methanol, acetonitrile, the content of each deuterated internal standard is 50ng / mL, water 20mL, methanol 40mL , acetonitrile 40mL; diluent is made up of methanol, acetonitrile and water, water 20mL, methanol 40mL, acetonitrile 40mL; organic phase is methanol: acetonitrile=1: 1, aqueous phase is 5mmol / L ammonium formate-0.2% formic acid.

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PUM

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Abstract

The invention discloses a kit for determining four antifungal drugs in a dry blood spot at the same time, comprising extracting solution, diluent, a mobile phase, a standard substance and a quality control material; the standard substance contains ketoconazole, voriconazole, itraconazole and posaconazole; the extracting solution is composed of an isotope internal standard, water and an organic solvent; the organic solvent is methanol and / or acetonitrile; and the mobile phase comprises an organic phase and an aqueous phase, the organic phase is methanol and / or acetonitrile, and the aqueous phase is ammonium formate / acetate-formic / acetic acid aqueous solution. The invention also discloses a method for determining the four antifungal drugs in the dry blood spot at the same time by using the kit. The kit disclosed by the invention is applicable to simultaneous determination of contents of the four antifungal drugs in a dry blood spot sample, sample detection specificity is high, sensitivity is high, and stability is good.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a kit for simultaneously measuring four antifungal drugs in dried blood spots and an application thereof. Background technique [0002] In recent years, the probability of human infection with fungi has increased significantly, mainly due to organ transplantation, immunosuppressive therapy, and the increase in AIDS populations. At present, the most widely used antifungal drugs in clinical practice mainly include ketoconazole, voriconazole, posaconazole and itraconazole. These four antifungal drugs have the advantages of strong antibacterial activity, broad antibacterial spectrum and few adverse reactions. It is used for the treatment of superficial and deep fungal diseases and has good curative effect. [0003] Despite great advances in the effectiveness of antifungal drugs, fungal infections remain associated with high morbidity and mortality. At the same time, due to the sig...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 李士敏袁京群吴筱丹葛志伟朱亚尔李家睿
Owner 易达精准(杭州)科技有限公司
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