tev protease mutant, gene, biological material, preparation method, reagent or kit and application

A technology of biomaterials and mutants, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problems of protease activity and stability reduction, low yield and water solubility, etc., and achieve improved Effects of activity and stability, improved water solubility, good stability and specificity
CN109055339BActive Publication Date: 2020-09-01生工生物工程(上海)股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
生工生物工程(上海)股份有限公司
Publication Date
2020-09-01

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Abstract

The invention provides a TEV protease mutant, a gene, a biomaterial, a preparation method, a reagent, or a reagent kit and application, and relates to the technical field of genetic engineering. Compared with a protease mutant having an amino acid sequence shown as SEQ ID NO.1, the TEV protease mutant is mutated into at least seven amino acid residues, for example 17th site is mutated into S fromT, the 56th site is mutated into V from L, the 68th site is mutated into D from N, the 77th site is mutated into V from I, the 135th site is mutated into G from S, the 219th site is mutated into V from S, and the 233th site is mutated into H from Q. The TEV protease mutant has favorable enzymatic activity, stability and specificity. The gene, the biomaterial and the preparation method of the TEV protease mutant, or the reagent or the reagent kit containing the TEV protease mutant, the application of the technical scheme, and the TEV protease mutant are all based on the same invention conception.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a TEV protease mutant, gene, biological material, preparation method, reagent or kit and application. Background technique

[0002] Prokaryotic expression is widely used in bioengineering. There are countless sources of genes expressed by prokaryotic expression systems, covering research directions in many fields, including protein purification, localization, and functional analysis. According to different expression systems, it can be divided into E. coli expression system, Bacillus subtilis expression system, Streptomyces expression system and so on. Among them, the Escherichia coli expression system is the most mature technology and the most widely used because of its simple culture method, clear expression background, and low expression cost.

[0003] However, the Escherichia coli expression system lacks enzymes, cofactors and other substances unique to the post-translatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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