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A kind of wound healing material and preparation method thereof

A technology for healing materials and wounds, applied in bandages, medical science, etc., can solve the problems of easy degradation of growth factors, high price, low utilization rate, etc., to achieve no toxic side effects, improve stability, and promote the effect of blood vessel formation.

Active Publication Date: 2021-06-22
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, because growth factors are easy to degrade, have a short half-life, and are easy to inactivate, they cannot act on the wound site for a long time to produce biological effects, and their biological activity is dose-dependent, so they need to be used repeatedly in large doses, which are expensive and have low utilization.

Method used

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  • A kind of wound healing material and preparation method thereof
  • A kind of wound healing material and preparation method thereof
  • A kind of wound healing material and preparation method thereof

Examples

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preparation example Construction

[0028] An embodiment of the present invention provides a preparation method of a wound healing material, which includes:

[0029] S1. Mix the heparin salt buffer solution with the dopamine polymer, perform condensation reaction, and then dialyze and freeze-dry to obtain the heparin-dopamine polymer.

[0030] That is to say, through the strong anti-oxidation of dopamine, it can reduce the ROS during the wound healing period, thereby promoting the polarization of macrophages, and through the condensation of heparin and dopamine, it can effectively improve the speed of wound healing.

[0031] In order to enable the carboxyl group in heparin to combine more stably with the amino group of dopamine to form an amide bond and have a higher potency, in a preferred embodiment of the present invention, the heparin salt is preferably heparin salt after the carboxyl group has been activated.

[0032] Since dopamine has a strong anti-oxidation property and is easily oxidized by oxygen in th...

Embodiment 1

[0073] Heparin-dopamine polymers are prepared by:

[0074] Dissolve 50mg of heparin sodium in 20ml of PBS, add 3.13mg of EDC and 2.15mg of NHS (so that the molar ratio of heparin, EDC and NHS is 1:1:1), stir well under nitrogen protection for half an hour, then add 32.13 mg of dopamine, stirred and reacted for 2 hours with a stirrer, then took the reaction solution and put it into a dialysis bag, and dialyzed for 2 days, changing the water every 6-12 hours. The dialysate was freeze-dried in a freeze dryer to obtain a heparin-dopamine polymer.

Embodiment 2

[0076] The initial reaction product was prepared by:

[0077] Dissolve 40mg of CTAB in 1ml of pure water, take 0.5mg of Fe 3 o 4 Nanoparticles were dissolved in 0.1ml of chloroform, and then added dropwise to 1ml of CTAB solution, and the reaction was stable for 30 minutes with stirring bar. Then, it was heated in an oil bath at 70° C. for 10 minutes, and the chloroform was removed to make it clear. The obtained liquid was divided into tubes for centrifugation, and the solid obtained after centrifugation was washed with water three times to remove excess CTAB, and the Fe after CTAB treatment was obtained. 3 o 4 magnetic nanoparticles.

[0078] will give 0.5mg of Fe 3 o 4 The magnetic nanoparticles were added to 1 ml of heparin-dopamine aqueous solution with a concentration of 5 mg / ml, reacted for 2 hours, and then centrifuged at 12000 rpm to obtain the initial reaction product, wherein the heparin-dopamine was prepared by the method provided in Example 1.

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Abstract

The invention relates to a wound healing material and a preparation method thereof, and relates to the field of biomedical materials. The preparation method of the wound healing material comprises: mixing heparin salt buffer solution with dopamine polymer, dialysis and freeze-drying after condensation reaction to obtain heparin-dopamine polymer, and the heparin salt is the heparin salt after carboxyl activation. The heparin-dopamine polymer is modified onto magnetic nanoparticles to obtain an initial reaction product. The wound healing material is obtained by grafting the growth factor to the initial reaction product. The preparation method is controllable and the preparation process is relatively simple. The prepared wound healing material can improve the stability of the growth factor and realize the Long-term release, no toxic side effects, and fully participate in the process of wound healing, reduce inflammation, and promote wound healing.

Description

technical field [0001] The invention relates to the field of biomedical materials, and in particular to a wound healing material and a preparation method thereof. Background technique [0002] Growth factors are a kind of signaling molecules secreted by cells, similar to hormones. Most of them are peptide substances, which can regulate cell growth and differentiation, and can promote cell proliferation, collagen proliferation, granulation tissue formation, and tissue differentiation. . [0003] However, because growth factors are easy to degrade, have a short half-life, and are easy to inactivate, they cannot act on the wound site for a long time to produce biological effects, and their biological activity is dose-dependent, so they need to be used repeatedly in large doses, which are expensive and have low utilization. . Therefore, it is particularly important to improve the stability and utilization of growth factors by constructing a slow-release system. Contents of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0019A61L26/0023A61L26/0061A61L26/0066A61L2300/41A61L2300/414A61L2300/604C08L79/04C08L5/10
Inventor 吴疆何华成肖健朱俊逸宣暄程超群李校堃
Owner WENZHOU MEDICAL UNIV