Novel fish skin source oral repair membrane and preparation method thereof

A technology for oral prosthesis and fish skin, which is applied in medical science, prosthesis, etc., can solve the problems of poor mechanical properties of aquatic organisms collagen, limited human skin repair, weak anti-degradation ability, etc., achieves low cross-linking toxicity, and eliminates potential safety hazards. , the effect of low price

Inactive Publication Date: 2018-12-25
QINGDAO MARINE BIOPHARMACEUTICAL RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, aquatic biocollagen has disadvantages such as poor mechanical properties, poor thermal stability, and weak resistance to degradation, which greatly limit its further development in medical materials.
Currently, acellular dermal matrix cross-linked with glutaraldehyde (GA) has been studied, which has excellent mechanical properties, thermal stability and resistance to degradation, but due to the toxicity of glutaraldehyde (GA), it is not used for human skin repair. restricted

Method used

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  • Novel fish skin source oral repair membrane and preparation method thereof
  • Novel fish skin source oral repair membrane and preparation method thereof
  • Novel fish skin source oral repair membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Pretreatment: take sturgeon skin as raw material, use physical method to remove the fish meat residue, clean it up, degrease with enzymatic method, decolorize with hydrogen peroxide, crush cells and remove impurities with the aid of alkali method and ultrasonic to obtain fish skin Acellular dermal matrix (FSADM).

[0037] (2) Preparation of EDC / NHS cross-linking agent: prepare a solution in which 40 mM EDC and NHS are mixed at a ratio of 2:1.

[0038] (3) FSADM cross-linking: FSADM was trimmed into squares with a size of 1cm*1cm, soaked in EDC / NHS cross-linking agent, and ultrasonicated in an ice bath for 20s, intermittently for 2min, repeated 5 times. Then place the FSADM soaked in the cross-linking agent in a magnetic stirrer and stir for 6 hours to make it stir at low temperature, wherein the power of the magnetic stirrer is 250W, and the rotation speed is 100 rpm.

[0039] (4) Sterile washing: Soak and wash the cross-linked FSADM sample in sterile electrolyte w...

Embodiment 2

[0043] (1) Pretreatment: Using catfish skin as raw material, remove the fish meat residue by physical method, clean it, degrease it by enzymatic method, decolorize it with hydrogen peroxide, break the cells with the aid of alkaline method and ultrasonic to remove impurities, and get the degreased fish skin. Cellular dermal matrix (FSADM).

[0044] (2) Preparation of EDC / NHS cross-linking agent: prepare a solution in which 35 mM EDC and NHS are mixed at a ratio of 3:1.

[0045] (3) FSADM cross-linking: FSADM was trimmed into squares with a size of 1cm*1cm, soaked in EDC / NHS cross-linking agent and ultrasonicated in an ice bath for 15s, with an interval of 1min, repeated 6 times. Then place the FSADM soaked in the cross-linking agent in a magnetic stirrer and stir for 7 hours to make it stir at low temperature, wherein the power of the magnetic stirrer is 250W, and the rotation speed is 100 rpm.

[0046] (4) Sterile washing: Soak and wash the cross-linked FSADM sample in steril...

Embodiment 3

[0050] (1) Pretreatment: use pangasius skin as raw material, use physical method to remove the fish meat residue, clean it, degrease with enzymatic method, decolorize with hydrogen peroxide, crush cells and remove impurities with the aid of alkaline method and ultrasonic to obtain fish skin Acellular dermal matrix (FSADM).

[0051] (2) Preparation of EDC / NHS cross-linking agent: prepare a solution in which 30 mM EDC and NHS are mixed at a ratio of 2.5:1.

[0052](3) FSADM cross-linking: FSADM was trimmed into squares with a size of 1cm*1cm, soaked in EDC / NHS cross-linking agent and ultrasonicated in an ice bath for 15s, with an interval of 1min, repeated 7 times. Then place the FSADM soaked in the cross-linking agent in a magnetic stirrer and stir for 10 h to make it stir at low temperature, wherein the power of the magnetic stirrer is 250W, and the rotating speed is 100 rpm.

[0053] (4) Sterile washing: soak and wash the cross-linked FSADM sample in sterile electrolyte wate...

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Abstract

The invention discloses a novel fish skin source oral repair membrane and a preparation method thereof. Fish skin acellular dermal matrixes and EDC / NHS cross-linking agents are cross-linked to obtainthe oral repair membrane. The preparation method of the oral repair membrane includes the steps: taking a fresh fish skin as a raw material, removing fish meat, cleaning the fish skin without the fishmeat, performing enzymatic degreasing and hydrogen peroxide discoloring, disrupting cells by an alkaline method and an ultrasonic auxiliary mode, and removing impurities to obtain the fish skin acellular dermal matrixes; preparing the EDC / NHS cross-linking agents; soaking the fish skin accellular dermal matrixes into the EDC / NHS cross-linking agents, repeatedly performing ice bath ultrasonic every 1-2min, and placing the fish skin acellular dermal matrixes into a magnetic stirring apparatus to stir the matrixes to obtain a cross-linked oral repair membrane; washing the cross-linked oral repair membrane under the sterility condition, and drying, packaging and sterilizing the washed cross-linked oral repair membrane. The fish skin source oral repair membrane is excellent in mechanical property and heat stability, high in degradation resistance and high in safety.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a novel oral repair membrane derived from fish skin and a preparation method thereof. Background technique [0002] Tissue Engineering is a new discipline emerging in recent years, with good development prospects and broad application markets. Acellular dermal matrix (ADM) is an excellent biomedical material and has been widely used because of its good biocompatibility. The current acellular dermal matrix is ​​mainly derived from terrestrial animals. However, due to the spread of diseases such as foot-and-mouth disease and mad cow disease and the restrictions of religious beliefs, it is becoming more and more important to find medical collagen from aquatic organisms as an alternative source. [0003] The main component of the acellular dermal matrix is ​​collagen, and studies have confirmed that aquatic biological collagen can be utilized ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/362A61L27/3641A61L27/3687A61L27/3691
Inventor 李八方侯虎王月坤王长伟刘亚茹
Owner QINGDAO MARINE BIOPHARMACEUTICAL RES INST
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