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Soil Streptomyces costaricanus with biofertilizer and biopesticide potential

A bio-pesticide and bio-fertilizer technology is applied in the field of microorganisms to achieve the effects of pollution-free drug resistance, difficulty in drug resistance, and reduced hatching rate

Active Publication Date: 2018-12-25
陆丰植物龙生态农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of microorganisms does not limit where they come from. Even if they are of the same species in the same genus, there may still be a high degree of difference between different strains

Method used

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  • Soil Streptomyces costaricanus with biofertilizer and biopesticide potential
  • Soil Streptomyces costaricanus with biofertilizer and biopesticide potential
  • Soil Streptomyces costaricanus with biofertilizer and biopesticide potential

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Isolation of Streptomyces CP99

[0042] 1. Isolation and cultivation of soil Streptomyces

[0043] 1. Biological materials and culture medium: After sampling and diagnosis from a tomato field with severe disease in Tanzhou District, Zhongshan City, Guangdong Province, it was found that the root-knot nematodes were seriously harmful, and the vascular bundles were yellowed. According to the Koch criteria, it was determined to be Fusarium sp. Tomato wilt disease and root-knot nematode common hazards caused by bacteria, the collected plant roots and rhizosphere soil were separated to obtain samples; the separation medium was potato sucrose medium, and the ingredients were as follows: potato: 200g, sucrose: 20g , agar: 17g, filtered water: 1000ml;

[0044]2. Sample treatment: Take 1g sample, put it into 9mL sterile water, shake vigorously and let it stand for 10min, then dilute the sample 1:10, take 100μL dilution, and evenly spread it on the separation medium pl...

Embodiment 2

[0074] Embodiment 2: the preparation of streptomyces costaricanus (Streptomyces costaricanus) CP99 fermented liquid

[0075] 1. Activation culture of strains: move the isolated and preserved Streptomyces CP99 to potato sucrose plate medium (potatoes: 200g, sucrose: 20g, agar: 17g, filtered water: 1000ml) and cultivate them in an incubator at 30°C for 5 days;

[0076] 2. Fermentation culture of bacterial strains: take the activated Streptomyces CP99 on the potato sucrose plate medium and inoculate it into the potato sucrose liquid medium (100mL medium / 250mL Erlenmeyer flask, formula: potato: 200g, sucrose: 20g, filtered water : 1000ml), cultured in a shaker at 180rpm / min at 30°C for 5 days to obtain a Streptomyces costaricanus CP99 fermentation broth.

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Abstract

The invention relates to soil Streptomyces costaricanus with biofertilizer and biopesticide potential and belongs to the technical field of microorganisms. The soil Streptomyces costaricanus has a class name of Streptomyces costaricanus CP99 collected on July, third, 2018 under GDMCC No. 60404. The Streptomyces costaricanus CP99 can effectively inhibit plant fungal diseases caused by Fusarium (such as Fusarium oxysporum f. sp. cucumerinum and Fusarium oxysporum f. sp. niveum), Phytophthora (such as Phytophthora fruit rot germs), Pythium aphanidermatum, and Rhizoctonia solani, and can reduce egg hatching rate for root-knot nematodes and reduce damage level of root-knot nematodes. The Streptomyces costaricanus CP99 effectively promote growth of roots owing to not IAA but unknown root growthpromotion factors. The Streptomyces costaricanus CP99 has the potential of being developed into biopesticides (including growth regulators and biocontrol agents).

Description

technical field [0001] The invention relates to a soil streptomyces, in particular to a soil streptomyces with the potential of biological fertilizer and biological pesticide, the soil streptomyces has the functions of promoting plant root growth, plant growth and preventing and controlling plant soil-borne fungal diseases and nematode diseases The invention belongs to the technical field of microorganisms. Background technique [0002] The biological causes of plant diseases include four major pathogens: fungi, bacteria, nematodes, and viruses. Among them, the types of fungal diseases are the most, and the severe ones include: soil-borne diseases caused by Fusarium spp. such as fusarium wilt of melons, root rot, banana yellow leaf disease, etc.; Pythium ( Pythium spp.) caused by papaya blight; Rhizoctonia solani (Rhizoctonia solani) and so on. Nematode diseases are difficult to control due to their wide range of hosts and moderate ecological characteristics of hiding in p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P5/00A01P21/00C12R1/465
CPCA01N63/10C12N1/20C12N1/205C12R2001/465
Inventor 蔡凯荣郑淑娴赵基荃
Owner 陆丰植物龙生态农业发展有限公司