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Application of a kind of kaempferol (4-o-methyl) glucoside compound in anti-insect agents

A technology of glucoside and kaempferol, which is applied in the fields of application, insecticide, antineoplastic, etc., and can solve the problems of few reports on insect resistance activity

Active Publication Date: 2021-03-05
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the anti-insect activity of kaempferol derivatives

Method used

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  • Application of a kind of kaempferol (4-o-methyl) glucoside compound in anti-insect agents
  • Application of a kind of kaempferol (4-o-methyl) glucoside compound in anti-insect agents
  • Application of a kind of kaempferol (4-o-methyl) glucoside compound in anti-insect agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Application of glycosyltransferase recombinant vector PRS425m-IfUGT and O-methyltransferase recombinant vector PXW06F-IfOMT to realize glycosyl methylation modification of kaempferol

[0024] 1.1 Instruments and materials

[0025] Bacterial strains and carriers used in the experiment: Isaria fumosorosea ACCC37775 and yeast S.cerevisiae BJ5464-NpgA, yeast expression vectors PRS425m-IfUGT and PXW06F-IfOMT, all derived from the Institute of Biotechnology, Chinese Academy of Agricultural Sciences, by the present invention The human laboratory is self-preserved. The reagents used in the experiment were domestic analytically pure products. Culture medium: Escherichia coli medium is LB medium (1% peptone, 0.5% yeast extract, 1% NaCl, pH7.0). SC- - Leu-deficient medium (1% glucose, 6.7% Difco TM Yeast Nitrogen Base w / o Amino Acids, -Leu / -Trp DO Supplement). YPD medium (1% yeast extract, 2% Peptone peptone, 2% glucose). YPD low-sugar medium (1% yeast extract, 2%...

Embodiment 2

[0034] Example 2 Extraction, separation and structural identification of compound 1-3

[0035] 2.1 Instruments and materials

[0036] The solvents chloroform, methanol, and ethyl acetate used in the experiment were produced by Beijing Chemical Reagent Factory, all of which were of analytical grade. Chromatographically pure acetonitrile was produced by Fisher Chemical Company. Column chromatography silica gel was produced by Qingdao Ocean Chemical Factory (200-300 mesh). Thin-layer silica gel chromatography plates were produced by Yantai Huangwu Silica Gel Development and Experimental Factory. BUCHI R-300 rotary evaporator, BUCHI I-300 touch central control unit, BUCHI V-300 PTFE membrane vacuum pump, BUCHI B-300 electric constant temperature water bath, all produced by Swiss BUCHI company. The low-temperature coolant circulation pump is produced by Shanghai Zhixin Experimental Instrument Technology Co., Ltd. The semi-preparative high-performance liquid chromatograph is Agi...

Embodiment 3

[0046] The solubility test of embodiment 3 kaempferol and its glycoside derivatives

[0047] 3.1 Purpose of the experiment

[0048] Determining the effect of sugar methylation on the solubility of kaempferol in different solvents.

[0049] 3.2 Experimental method

[0050] Weigh 1 mg of kaempferol and compound 1 respectively, place in 1ml of different solvents at 25°C±2°C, shake vigorously for 30 seconds every 5 minutes; observe the dissolution within 30 minutes, if no solute particles or liquid When dripping, it is considered to be completely dissolved. The selected solvents are distilled water, methanol, ethanol, etc.

[0051] 3.3 Experimental results and analysis

[0052] Table 4 shows the solubility of kaempferol and its glycosylated derivatives (compound 4) and sugar methylated derivatives (compound 1) in different solvents.

[0053] Table 4 Solubility of Kaempferol and Compound 1 in Different Solvents

[0054] solvent Kaempferol Compound 4 Compound 1...

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Abstract

The invention discloses a kind of kaempferol (4- O ‑Methyl) glucoside compounds are used as insecticides. In the biological activity test, this compound shows insecticidal activity, which is not possessed by its aglycone. At the same time, compared with the aglycon, the water-soluble The stability is significantly enhanced; compared with unmethylated kaempferol glucoside, the stability of this compound is significantly enhanced, and it has a wide range of application values.

Description

technical field [0001] The invention belongs to the field of insecticides, and in particular relates to the use of kaempferol-(4-O-methyl)glucoside compounds in insecticides. Background technique: [0002] Kaempferol is a common natural flavonoid plant secondary metabolite, which widely exists in the roots, leaves and fruits of many plants. Kaempferol has anti-oxidation, anti-inflammation, inhibition of tumor growth, protection of liver cells, inhibition of platelet aggregation and adhesion, and other activities, so it has attracted widespread attention. However, kaempferol is only slightly soluble in water, and has poor metabolic stability in mammals. It is easily affected by various factors from organisms and the outside world, resulting in self-degradation and reduced activity, which greatly hinders its application. Glucosylation and methylation are common modification reactions in organisms, which can change the physical and chemical properties of compounds such as wate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P7/04A61K31/7048A61P35/00
CPCA01N43/16A61K31/7048A61P35/00
Inventor 徐玉泉张礼文王辰顿宝庆
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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