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Paenibacillus polymyxa and its spore suspension, microbial seedling culture substrate and preparation method thereof

A technology of polymyxa-like spores and seedling substrates, applied in the field of microorganisms, can solve problems such as lack of control measures, and achieve the effects of improving immunity, increasing yield, and inhibiting formation

Active Publication Date: 2021-11-16
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is still a lack of effective pepper blight-resistant varieties in production. The prevention and control of pepper blight mainly depends on chemical control, but pathogenic bacteria have developed resistance to most chemical pesticides, so there is still a lack of effective control measures in production.

Method used

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  • Paenibacillus polymyxa and its spore suspension, microbial seedling culture substrate and preparation method thereof
  • Paenibacillus polymyxa and its spore suspension, microbial seedling culture substrate and preparation method thereof
  • Paenibacillus polymyxa and its spore suspension, microbial seedling culture substrate and preparation method thereof

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Embodiment 1

[0035] The invention provides a Paenibacillus polymyxa, which is Paenibacillus polymyxa Paenibacillus polymyxaPp-HG15-7 was deposited in the "Common Microbiology Center of China Microbial Culture Collection Management Committee" on July 27, 2017, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. .14470. Paenibacillus polymyxa Paenibacillus polymyxa . The Paenibacillus polymyxa is isolated and screened from the wheat rhizosphere, and can be cultivated by using TSA medium. details as follows:

[0036] Screening of bacterial strains Collect 1 g of the root of wheat belt soil with excellent growth and healthy growth, grind it with a mortar, and dilute it to 10,000 times with sterile water, and spread the diluted mixture evenly on a 1 / 10 TSA medium plate , placed upside down in a constant temperature incubator at 30°C and cultivated for 48 hours. After the strains grew out, bacterial colonies with different sizes, c...

Embodiment 2

[0041] This embodiment provides a Paenibacillus polymyxa spore suspension, which comprises a sterile magnesium sulfate solution and the above-mentioned Paenibacillus polymyxa Pp-HG15-7 spores uniformly dispersed in the sterile magnesium sulfate solution. Wherein, in the suspension of Paenibacillus polymyxa spores, the concentration of the spores of Paenibacillus polymyxa Pp-HG15-7 provided in Example 1 is 2×10 8 CFU / mL.

[0042] This embodiment also provides a method for preparing the above-mentioned Paenibacillus polymyxa spore suspension, which includes the following steps: the Paenibacillus polymyxa strains isolated and screened from the wheat rhizosphere are cultured on a TSA medium. Bacillus Pp-HG15-7 strain; the Paenibacillus polymyxa Pp-HG15-7 strain was inoculated on the fermentation medium, and fermented and cultivated at 32°C for 78 h to obtain a Paenibacillus polymyxa fermentation broth, Wherein, the formula of the fermentation medium includes 25 g of bean dregs, ...

Embodiment 3

[0046] This embodiment provides a Paenibacillus polymyxa spore suspension, which is basically the same as the Paenibacillus polymyxa spore suspension provided in Example 2, except that in this embodiment In the Paenibacillus polymyxa spore suspension provided, the concentration of the spores of the Paenibacillus polymyxa Pp-HG15-7 provided in Example 1 is 1.5×10 8 CFU / mL.

[0047] This embodiment also provides a preparation method of the above-mentioned Paenibacillus polymyxa spore suspension, which is basically the same as the preparation method provided in Example 2, except that the formula of the fermentation medium used in this embodiment includes bean dregs 22 g, NaCl 0.6 g, 0.25mol / L KCl 12 mL, 2mol / L MgCl 2 6 mL, 18 mL of 1mol / L glucose and 1000 mL of distilled water, and the pH value of the fermentation medium was 6.8; the fermentation temperature of the Paenibacillus polymyxa strain was 30°C, and the fermentation time was 84 h; The concentration of bacterial magne...

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Abstract

The invention provides a Paenibacillus polymyxa, which is Paenibacillus polymyxa Paenibacillus polymyxa Pp-HG15-7, the deposit number is CGMCC No.14470. The invention provides a Paenibacillus polymyxa spore suspension, which comprises a sterile magnesium sulfate solution and the above-mentioned Paenibacillus polymyxa spores uniformly dispersed in the sterile magnesium sulfate solution. The present invention also provides a microbial seedling-growing substrate containing the above-mentioned Paenibacillus polymyxa spore suspension and a preparation method thereof. The above-mentioned Paenibacillus polymyxa and its spore suspension can prevent Phytophthora and prevent the invasion of pathogenic bacteria. Therefore, the above-mentioned microbial seedling substrate can improve the immunity of vegetables, prevent and control the occurrence of epidemic diseases, promote plant growth, and significantly improve root activity. Helps increase production.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a Paenibacillus polymyxa and its spore suspension, a microbial seedling-raising substrate and a preparation method thereof. Background technique [0002] Plant Growth Promoting Rhizobacteria (PGPR for short) refers to the presence in the plant rhizosphere environment, through the direct effects of producing plant hormones, dissolving inorganic phosphorus, and indirect effects such as competing for ecological niches and inducing plant system resistance. A class of beneficial bacteria that grow on plants. In recent years, a large number of studies have shown that PGPR strains can effectively colonize plant roots and rhizosphere soils, have significant effects on growth promotion, disease prevention and production increase, and at the same time regulate the micro-ecological environment of plant rhizosphere soils and alleviate ecological pollution. It is one of the effectiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G1/00A01G24/20C12R1/01
CPCA01G24/20C05D1/02C12N1/20C12N1/205C12R2001/01C05D5/00C05F5/002C05G5/23
Inventor 申顺善朴凤植张涛张忠良
Owner HENAN AGRICULTURAL UNIVERSITY