Bacillus siamensis and application thereof

A bacillus and bacterial suspension technology, applied in the field of microorganisms, can solve the problems of less research on Bacillus siamese and less research on active ingredients, and achieve the effects of reducing aflatoxin content, reducing infection, and improving degradation rate

Active Publication Date: 2019-01-01
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on Bacillus siamese, most of which focus on the field of crop disease control and animal feed; there are not many studies on its active ingredients

Method used

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  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof
  • Bacillus siamensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Sample Collection, Strain Isolation and Screening

[0031] 1. Sample collection time and place

[0032] On the morning of May 5, 2017, soil samples of peanut fields were collected from the experimental base of Shandong Peanut Research Institute (Wangcheng Town, Laixi City, Qingdao City).

[0033] 2. Sample processing, strain isolation and screening

[0034] Take 1 g of the collected soil sample and suspend it in 10 mL of sterile water in an ultra-clean bench, shake and dilute to prepare a soil suspension, and then dilute 100 times with sterile distilled water. Take 100 μL of the suspension and spread it on the LB solid plate, and place it at 37 degrees Celsius for cultivation. After 3 days, the grown colony is streaked and purified on the LB solid plate. After 3 times of streaking and purification, a strain is obtained, numbered A2025 .

Embodiment 2

[0035] Identification of embodiment 2 bacterial strain A2025

[0036] According to the method described in "Berger Bacteria Identification Handbook" (Eighth Edition), the morphological characteristics and physiological and biochemical characteristics of strain A2025 were identified, and the specific results were as follows:

[0037] 1. Morphological characteristics: The single colony of strain A2025 is raised on LB medium, milky white, opaque ( figure 1 ), after 2 days of culture at 37°C, the colony was about 4-6mm, and wrinkles appeared on the surface of the colony after 3 days of culture.

[0038] 2. Physiological and biochemical characteristics: Oxidase activity is negative, can grow at 4-54°C, Gram staining is positive, can hydrolyze and utilize casein, gelatin and starch, but cannot utilize Tween 40 and Tween 80.

[0039] 3. 16S rRNA gene analysis

[0040] The bacterial genomic DNA of A2025 was extracted, and PCR amplification was carried out using 16S rRNA gene univers...

Embodiment 3

[0042] The cultivation of embodiment 3 Bacillus siamese A2025

[0043] The medium of Bacillus siamese A2025 is LB medium: 10g peptone, 10g sodium chloride, 5g yeast extract, 1000mL water; it can grow at 4-54°C, the optimal culture temperature is 26-37°C, and it can grow at pH 5-9 growth, the optimal pH is 7-8. It can also grow in GY medium, GY liquid medium: glucose 20g, yeast powder 5g, water 1000mL; GY solid medium: glucose 20g, yeast powder 5g, agar 20g, water 1000mL.

[0044] Take out the frozen Bacillus siamica A2025 from the refrigerator at -80°C, inoculate 50 μL of the Bacillus siamica A2025 bacterial solution into a 5 mL LB liquid medium test tube in the ultra-clean workbench, and cultivate the activated strain for 12 hours. Insert 500 μL of activated bacterial solution into 100 mL of LB liquid medium, shake and culture at 37°C for 48 hours, and the concentration of the bacterial solution is 1.6×10 8 cfu / mL.

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Abstract

The invention discloses a bacillus siamensis and application thereof and belongs to the technical field of microorganisms. The bacillus siamensis provided by the invention is bacillus siamensis A2025and is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 12, 2018; the preservation number is CGMCC NO: 15611 and the address is #3, No. 1 Yard, West Beichen Road,Chaoyang District, Beijing; and the preservation requesting unit is Shandong Peanut Research Institute. The bacillus siamensis A2025 obtained by the invention can be used for remarkably inhibiting thegrowth of aspergillus flavus and can be used for efficiently degrading aflatoxin.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of bacillus siamese and its application. Background technique [0002] Aspergillus flavus is a common saprophytic fungus belonging to the class of Deuteromycetes. Aspergillus flavus can produce aflatoxin, which can not only cause poisoning and death in poultry and livestock, but also cause cancer. The methods commonly used to remove aflatoxins are mainly physical and chemical methods. Microbial detoxification method has become a research hotspot in recent years. This method mainly uses bacteria, fungi and other microorganisms and their metabolites to remove contaminated AFB from food. 1 , the detoxification method has the advantages of no pollution to raw materials, high specificity, avoiding the regeneration of toxins, mild degradation conditions, strong specificity and high detoxification efficiency, so it is an efficient and safe detoxification me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B9/28A23L5/20C12R1/07
CPCA23B9/28A23L5/28C12N1/205C12R2001/07
Inventor 王明清于丽娜孙杰张初署毕洁张建成杨伟强尹登科
Owner SHANDONG PEANUT RES INST
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