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Application of mulberry leaf extract in preparation of feed antioxidant, feed and preparation method of feed

A technology of mulberry leaf extract and antioxidant, which is applied in the direction of food ingredients as antioxidants, food ingredients containing natural extracts, animal feed, etc. It can improve the economic and social benefits of mulberry leaves, improve the utilization structure of mulberry leaves, and achieve the best effect of anti-oxidation.

Inactive Publication Date: 2019-01-04
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the antioxidants used more in the feed industry are mainly synthetic antioxidants, including ethoxyquinoline (EQ), butylhydroxyanisole (BHA), butylated hydroxytoluene (BHT), etc., but there have been reports It has been shown that these antioxidants are carcinogenic and genotoxic to animals, and will lead to their residue and enrichment in fish products, causing food safety hazards and endangering human health
In February 2017, the European Union issued the G / SPS / EU / 190 Announcement of 2017: stop adding EQ to animal feed; at the same time, it was reported that the carcinogenic risk of adding BHT and BHA to animal feed was higher than that of EQ
Therefore, the disadvantages of these synthetic antioxidants will lead to an increase in the market's demand for antioxidants from natural sources, and the development of new natural feed antioxidants is of great significance
[0003] Mulberry planting has a long history in my country, and mulberry leaves are mainly used to raise silkworms, edible and used as medicine, but there is no report on the application of mulberry leaf extracts in feed antioxidation

Method used

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  • Application of mulberry leaf extract in preparation of feed antioxidant, feed and preparation method of feed
  • Application of mulberry leaf extract in preparation of feed antioxidant, feed and preparation method of feed
  • Application of mulberry leaf extract in preparation of feed antioxidant, feed and preparation method of feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Mulberry leaf extract, its preparation method comprises the following steps:

[0033] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;

[0034] (2) Mix mulberry leaf powder and ethyl acetate in a weight ratio of 1:9, mix and stir with a rotary agitator at room temperature, 700r / min for 8 hours, and filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper respectively , repeated 3 times to obtain a clear residue-free filtrate;

[0035] (3) The filtrate was placed in a rotary evaporator and distilled under reduced pressure to constant weight, and ethyl acetate was removed to obtain a mulberry leaf extract (ethyl acetate extract).

Embodiment 2

[0037] Mulberry leaf extract, its preparation method comprises the following steps:

[0038] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;

[0039] (2) Mix mulberry leaf powder and petroleum ether in a weight ratio of 1:9, mix and stir for 8 hours with a rotary agitator at room temperature, 700r / min, and filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper respectively, Repeat 3 times to obtain a clear residue-free filtrate;

[0040] (3) Place the filtrate in a rotary evaporator and distill it under reduced pressure to constant weight, remove the petroleum ether, and obtain the mulberry leaf extract (petroleum ether extract).

Embodiment 3

[0042] Mulberry leaf extract, its preparation method comprises the following steps:

[0043] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;

[0044] (2) Mix mulberry leaf powder and ethanol in a weight ratio of 1:9, mix and stir at room temperature with a rotary agitator at 700r / min for 8 hours, filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper, repeat 3 times to obtain a clear residue-free filtrate;

[0045] (3) The filtrate is placed in a rotary evaporator and distilled under reduced pressure to constant weight, and ethanol is removed to obtain a mulberry leaf extract (ethanol extract).

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Abstract

The invention provides an application of a mulberry leaf extract in preparation of a feed antioxidant, a feed and a preparation method of the feed. A preparation method of the mulberry leaf extract comprises: drying and pulverizing mulberry leaves, and then adding an organic solvent or water for extracting and filtering to obtain filtrate and filter residue; repeating the extraction for 1 to 3 times, combining the filtrates, distilling under reduced pressure to constant weight, thus obtaining the mulberry leaf extract. The prepared mulberry leaf extract is used to prepare a feed, especially afish feed, wherein the mulberry leaf extract accounts for 0.038-0.07% of the total mass of the fish feed, and the remaining ingredients are fish meal, soybean meal, flour, sunflower oil, calcium dihydrogen phosphate, vitamin additives and mineral additives. The preparation method comprises the following steps: adding the mulberry leaf extract to the sunflower oil, mixing uniformly, then adding theremaining ingredients, mixing uniformly, and preparing the mixture into granules. The mulberry leaf extract is used as the fish feed antioxidant, thereby avoiding the toxic effect of the conventionalsynthetic antioxidant on fishes, developing a new use of the mulberry leaves, and effectively utilizing the mulberry leaf resources.

Description

technical field [0001] The invention belongs to the field of feed, and in particular relates to the application of mulberry leaf extract in preparing feed antioxidants, feed and a feed preparation method. Background technique [0002] At present, the antioxidants used more in the feed industry are mainly synthetic antioxidants, including ethoxyquinoline (EQ), butylhydroxyanisole (BHA), butylated hydroxytoluene (BHT), etc., but there have been reports It has been shown that these antioxidants are carcinogenic and genotoxic to animals, and will lead to their residue and enrichment in fish products, causing food safety hazards and endangering human health. In February 2017, the European Union issued the 2017 G / SPS / EU / 190 Announcement: Stop adding EQ to animal feed; at the same time, it was reported that the carcinogenic risk of adding BHT and BHA to animal feed was higher than that of EQ. Therefore, the disadvantages of these synthetic antioxidants will lead to an increase in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A23K50/80A23K10/22A23K10/37A23K10/30A23K20/158A23K20/26A23K20/174A23K20/20
CPCA23L3/3472A23V2002/00A23K10/22A23K10/30A23K10/37A23K20/158A23K20/174A23K20/26A23K20/30A23K50/80A23V2200/02A23V2250/21Y02P60/87Y02A40/818
Inventor 李华涛罗岚张珊福袁志张荣美卢磊吴敏
Owner NEIJIANG NORMAL UNIV
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