Application of mulberry leaf extract in preparation of feed antioxidant, feed and preparation method of feed
A technology of mulberry leaf extract and antioxidant, which is applied in the direction of food ingredients as antioxidants, food ingredients containing natural extracts, animal feed, etc. It can improve the economic and social benefits of mulberry leaves, improve the utilization structure of mulberry leaves, and achieve the best effect of anti-oxidation.
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Embodiment 1
[0032] Mulberry leaf extract, its preparation method comprises the following steps:
[0033] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;
[0034] (2) Mix mulberry leaf powder and ethyl acetate in a weight ratio of 1:9, mix and stir with a rotary agitator at room temperature, 700r / min for 8 hours, and filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper respectively , repeated 3 times to obtain a clear residue-free filtrate;
[0035] (3) The filtrate was placed in a rotary evaporator and distilled under reduced pressure to constant weight, and ethyl acetate was removed to obtain a mulberry leaf extract (ethyl acetate extract).
Embodiment 2
[0037] Mulberry leaf extract, its preparation method comprises the following steps:
[0038] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;
[0039] (2) Mix mulberry leaf powder and petroleum ether in a weight ratio of 1:9, mix and stir for 8 hours with a rotary agitator at room temperature, 700r / min, and filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper respectively, Repeat 3 times to obtain a clear residue-free filtrate;
[0040] (3) Place the filtrate in a rotary evaporator and distill it under reduced pressure to constant weight, remove the petroleum ether, and obtain the mulberry leaf extract (petroleum ether extract).
Embodiment 3
[0042] Mulberry leaf extract, its preparation method comprises the following steps:
[0043] (1) drying the mulberry leaves to a constant weight in an air blast drying oven below 50°C, and pulverizing them through a 18-mesh sieve to obtain mulberry leaf powder;
[0044] (2) Mix mulberry leaf powder and ethanol in a weight ratio of 1:9, mix and stir at room temperature with a rotary agitator at 700r / min for 8 hours, filter the mixed solution with a Buchner funnel and a medium-speed qualitative filter paper, repeat 3 times to obtain a clear residue-free filtrate;
[0045] (3) The filtrate is placed in a rotary evaporator and distilled under reduced pressure to constant weight, and ethanol is removed to obtain a mulberry leaf extract (ethanol extract).
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