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A kind of composite scaffold and its preparation method and application

A composite scaffold and collagen technology, applied in prosthesis, tissue regeneration, medical science, etc., can solve the problem of less materials and achieve the effect of low cost, wide sources and high production efficiency

Active Publication Date: 2021-07-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nowadays, there are endless materials to restore the ability of cartilage repair, but there are few materials with the function of longitudinal non-destructive dynamic monitoring

Method used

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  • A kind of composite scaffold and its preparation method and application
  • A kind of composite scaffold and its preparation method and application
  • A kind of composite scaffold and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of composite support, is made of following raw material: mass / volume is 0.8% (g / mL) collagen protein solution (Col) 10mL, nano-microcrystalline cellulose (CNC) 50mg and modified superparamagnetic nano trioxide Iron (USPIO) 1.3 mg. The mass of USPIO accounts for 1% of the total mass of collagen and nanocrystalline cellulose.

[0037] The preparation method is as follows:

[0038] Take the collagen solution in a petri dish, add nano-microcrystalline cellulose, stir and mix evenly, freeze-dry at -80°C, immerse in 0.25wt% glutaraldehyde solution for 3 hours after freeze-drying, and wash with excess deionized water Remove excess glutaraldehyde to obtain scaffold A; disperse the modified superparamagnetic nano-ferric oxide in 2 mL of deionized water to obtain a suspension of ferric oxide, immerse the scaffold A in the ferric oxide suspension, The composite scaffold can be obtained by freeze-drying at -80°C, which is denoted as Col(0.8%) / CNC / 1%USPIO.

Embodiment 2

[0040] A kind of composite support, is made of following raw material: mass / volume is 0.5% (g / mL) collagen protein solution (Col) 10mL, nano-microcrystalline cellulose (CNC) 50mg and modified superparamagnetic nano trioxide Iron (USPIO) 1 mg. The mass of USPIO accounts for 1% of the total mass of collagen and nanocrystalline cellulose.

[0041] The preparation method is as follows:

[0042] Take the collagen solution in a petri dish, add nano-microcrystalline cellulose, stir and mix evenly, freeze-dry at -80°C, immerse in 0.25wt% glutaraldehyde solution for 3 hours after freeze-drying, and wash with excess deionized water Remove excess glutaraldehyde to obtain scaffold A; disperse the modified superparamagnetic nano-ferric oxide in 2 mL of deionized water to obtain a suspension of ferric oxide, immerse scaffold A in the suspension, and freeze at -80 °C. The composite scaffold can be obtained after drying, which is denoted as Col / CNC / 1% USPIO.

Embodiment 3

[0044] A kind of composite support, is made of following raw material: mass / volume is 0.5% (g / mL) collagen protein solution (Col) 10mL, nano-microcrystalline cellulose (CNC) 50mg and modified superparamagnetic nano trioxide Iron (USPIO) 2mg. The mass of USPIO accounts for 2% of the total mass of collagen and nanocrystalline cellulose.

[0045] The preparation method is as follows:

[0046] Take the collagen solution in a petri dish, add nano-microcrystalline cellulose, stir and mix evenly, freeze-dry at -80°C, immerse in 0.25wt% glutaraldehyde solution for 4 hours after freeze-drying, and then wash with excess deionized water Remove excess glutaraldehyde to obtain scaffold A; disperse the modified superparamagnetic nano-ferric oxide in 2 mL of deionized water to obtain a suspension of ferric oxide, immerse the scaffold A in the ferric oxide suspension, The composite scaffold can be obtained by freeze-drying at -80°C, which is denoted as Col / CNC / 2% USPIO.

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Abstract

The invention discloses a composite bracket as well as a preparation method and application thereof. The raw materials for the preparation of the composite scaffold include the following components in parts by weight: 1-100 parts of collagen, 1-100 parts of nano-microcrystalline cellulose and 1-10 parts of modified superparamagnetic nano-ferric oxide. The invention also discloses a preparation method of the composite bracket. The porous composite scaffold prepared by the method of the present invention has excellent mechanical strength properties, good biocompatibility, and good degradation performance. Nanoparticles can provide well-marked imaging functions, and non-destructive dynamic monitoring of the morphology of new cartilage. Collagen combined with nano-microcrystalline cellulose simulates an environment model similar to that of human bone, replacing the nutritional environment space required for cell growth and reproduction during cartilage defect repair, and rapidly repairing cartilage defect.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a composite bracket and its preparation method and application. Background technique [0002] Degeneration and trauma are common causes of articular cartilage damage. Due to the lack of blood vessels in cartilage tissue, chondrocyte proliferation is slow, and bone self-repair ability is weak. Even small injuries may aggravate the disease. Currently, there is no good treatment for cartilage. Transplantation and replacement of bone also have high requirements on the source. Despite surgical treatment, the regenerated hyaline cartilage cannot recover its connection function. Although various biomaterials that promote cartilage regeneration have been developed in cartilage tissue engineering in recent years, the material based on longitudinal non-destructive observation of the state of new cartilage is another one. difficulty. [0003] Collagen (Collagen, Col)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/02A61L27/50A61L27/56A61L27/58
CPCA61L27/025A61L27/20A61L27/24A61L27/50A61L27/56A61L27/58A61L2430/06C08L1/04
Inventor 郭瑞杨卫冯龙宝朱麒宇陈杰
Owner JINAN UNIVERSITY