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Separation and purification preparation method for extracting corosolic acid from loquat leaves

A technology for the separation and purification of corosolic acid, applied in the field of medicine and biology, can solve the problems of large loss of corosolic acid, long extraction and purification time, low purity of corosolic acid, etc., to achieve industrial application and extraction and purification efficiency High, good product quality effect

Active Publication Date: 2019-01-04
四川兴杰象药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of corosolic acid obtained by separation and purification by this method is low, and it needs to go through many times of impurity removal and repeated crystallization to obtain the finished product of corosolic acid with high purity. The time is long, and after many steps of impurity removal and repeated crystallization, the loss of corosolic acid will be large and the yield will not be high
More importantly, the existing extraction and separation methods of corosolic acid have not undergone decolorization treatment, and these solvent extraction methods tend to extract more chlorophyll, resulting in higher chroma of corosolic acid, Existing reports use activated carbon adsorption for decolorization, but this method will lead to low extraction efficiency and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method for separating and purifying corosolic acid from loquat leaves, comprising the following steps:

[0034] (1) Using loquat leaves as raw materials, vacuum drying at 40°C until the water content is below 5%, and then pulverized to a particle size of 100-200 μm with a pulverizer to obtain 10 kg of loquat leaf powder;

[0035] (2) The loquat leaf powder obtained in step (1) is placed in 4 times parts by weight of a 90% ethanol solution (the total weight of the ethanol solution is 40kg, containing 0.2kg formic acid), and the ultrasonic power is 40kHz, the stirring speed Ultrasonic extraction at 300r / min for 15min; after ultrasonic extraction is completed, the extract is filtered, and the extract is concentrated under reduced pressure to remove ethanol to obtain an extract; the conditions of reduced pressure concentration are: pressure -0.02MPa, temperature 55°C;

[0036] (3) Dissolve the extract with acetic acid with a mass concentration of 30%, the amount of a...

Embodiment 2

[0041] A preparation method for separating and purifying corosolic acid from loquat leaves, comprising the following steps:

[0042] (1) Using loquat leaves as raw materials, vacuum drying at 60°C until the water content is 2%, and then pulverized by a pulverizer to a particle size of 60-120 μm, to obtain 5 kg of loquat leaf powder;

[0043] (2) Put the loquat leaf powder obtained in step (1) into 6 times parts by weight of an 88% ethanol solution (the total weight of the ethanol solution is 30kg, containing 0.6kg formic acid), and the ultrasonic power is 50kHz, the stirring speed Ultrasonic extraction at 350r / min for 10min; after ultrasonic extraction is completed, the extract is filtered, and the extract is concentrated under reduced pressure to remove ethanol to obtain an extract; the conditions of reduced pressure concentration are: pressure -0.05MPa, temperature 65°C;

[0044] (3) Dissolve the extract with acetic acid with a mass concentration of 35%, the amount of acetic acid a...

Embodiment 3

[0049] A preparation method for separating and purifying corosolic acid from loquat leaves, comprising the following steps:

[0050] (1) Using loquat leaves as raw materials, vacuum drying at 50°C until the water content is 3%, and then pulverized to a particle size of 10-60 μm to obtain 1 kg of loquat leaf powder;

[0051] (2) The loquat leaf powder obtained in step (1) is placed in 5 times by weight of an ethanol solution with a mass concentration of 90% (the total weight of the ethanol solution is 5kg, containing 0.5kg formic acid), and the ultrasonic power is 45kHz, the stirring speed Ultrasonic extraction at 320r / min for 12min; after the ultrasonic extraction is completed, the extract is filtered, and the extract is concentrated under reduced pressure to remove ethanol to obtain the extract; the conditions of reduced pressure concentration are: pressure -0.03MPa, temperature 60℃;

[0052] (3) Dissolve the extract with acetic acid with a mass concentration of 32%. The amount of a...

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Abstract

The invention provides a separation and purification preparation method for extracting corosolic acid from loquat leaves, and belongs to the technical field of medical biology. The preparation methodcomprises the following steps of 1, preparing loquat leaf powder; 2, conducting ultrasonic extraction on the loquat leaf powder for 10-15 min, and after ultrasonic extraction is completed, removing ethyl alcohol through reduced pressure concentration to obtain an extract; 3, dissolving the extract, and afterwards, putting the extract in supercritical CO2 fluid for extraction; 4, adsorbing extracted separation liquid through macroporous adsorption resin, after adsorption is saturated, adopting ethyl alcohol for dissolution, and recycling ethyl alcohol through reduced pressure to obtain corosolic acid concentrated liquid; 5, adjusting the pH value of the corosolic acid concentrated liquid to 6-7, and conducting crystallization after standing to obtain a finished product of the corosolic acid. By means of the preparation method, the corosolic acid can be quickly and efficiently extracted; the simplified preparing technology is simple, the extracting and purifying time is greatly shortened, the production cost is reduced, and the prepared corosolic acid is high in purity and low in chromaticity.

Description

Technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a method for separating and purifying corosolic acid, an active component of plants, and in particular to a method for separating and purifying corosolic acid from loquat leaves. Background technique [0002] Corosolic acid, also known as 2α-hydroxyursolic acid, is a five-ring triterpenoid compound that exists in plants such as Lagerstroemia and Loquat. Its basic skeleton is the five-ring core of polyhydropine. Corosolic acid is a white amorphous powder, soluble in petroleum ether, benzene, chloroform and other organic solvents, insoluble in water, soluble in hot ethanol and methanol. Under anhydrous conditions, it will react with strong acid (sulfuric acid, phosphoric acid, perchloric acid), medium strong acid (trichloroacetic acid) or Lewis acid (zinc chloride, aluminum trichloride, antimony trichloride), and it will cause color reaction or fluorescence . Corosolic acid...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008Y02P20/54
Inventor 向家兵朱莉向加刚秦素华张宇王龙军
Owner 四川兴杰象药业有限公司
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