Applications of lactobacillin plnE

A technology of lactic acid bacteria and amino acids, applied in the field of genetic engineering, can solve the problems of limited production of lactic acid bacteria, large amount required for antibacterial, low activity, etc., to improve intestinal mucosal immunity, small molecular weight, and convenient artificial synthesis Effect

Inactive Publication Date: 2019-01-11
江苏傲农生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, ammonium sulfate precipitation, ethyl acetate and other methods were used to purify lactobacillus, but the yield of lactobacillus obtained was limited and the activity was not high
[0003] Patent CN106191044A discloses a genetic engineering method for increasing the yield of lactobacillus plnK, and patent CN107043769A discloses a genetic engineering method for increasing the yield of lactobacillus plnJ. These two patents have prepared lactobacillus plnK and plnJ, but found in the application , although these two lactic acid bacteria also have antibacterial effects, but the amount required for antibacterial is relatively large, that is, the cost required for antibacterial is relatively high

Method used

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  • Applications of lactobacillin plnE
  • Applications of lactobacillin plnE
  • Applications of lactobacillin plnE

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] Embodiment: the preparation of lactobacillus plnE

[0020] (1) Refer to the nucleotide sequence of the plnE gene of Gene Bank lactic acid bacteria (Gene Bank: X94434), use Primer Premier6 to design a pair of specific primers, plnE upstream primer F: 5'-CGC GGATCC ATGCTACAGTTTGAGAAGTT-3', plnE downstream primer R: 5'-CG GAATTC TTAACGAATACTTTTCAAAA-3', the designed primer sequence was sent to Shanghai Sangon Bioengineering Co., Ltd. for synthesis.

[0021] (2) The upstream primer F and downstream primer R of the plnE gene were used as amplification primers, and the lactic acid bacteria DNA was used as a template to perform PCR amplification on the plnE gene, and the PCR amplification products were identified by agarose gel electrophoresis.

[0022] (3) After identifying the PCR product by agarose gel electrophoresis, purify the target band by tangent; carry out double digestion with the purified plnE product and plasmid pET-32(+); take the digested PCR target fragment a...

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PUM

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Abstract

The invention relates to applications of lactobacillin plnE. The amino acid sequence of the lactobacillin plnE is as shown in SEQ ID NO.1. The lactobacillin plnE can be used for sterilization and forimproving the immunity of weaned piglets. Compared with the prior art, a genetic engineering technology is used to perform in vitro recombination expression on the lactobacillin plnE, so that the lactobacillin plnE has strong bactericidal action against bacteria such as escherichia coli, staphylococcus aureus, salmonella and pasteurella, can be used for raising pigs or poultry, and especially canreduce diarrhea rate, improve the intestinal function and improve intestinal mucosal immunity when applied to feeds for suckling piglets, weaned piglets, growing pigs or breeding pigs.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the application of a lactobacillus plnE. Background technique [0002] With the extensive use or even abuse of antibiotics, it has also produced many adverse effects, such as causing animal flora imbalance, drug resistance, and endangering human health. Therefore, it is of great significance to study additives that can improve the health of livestock and poultry, improve growth performance, and are safe and non-toxic and can replace antibiotics. Lactobacillus refers to the antibacterial protein produced by some lactic acid bacteria through ribosome synthesis during their metabolism. Lactobacillus has the advantages of non-toxicity, no side effects, no residue, low temperature storage resistance, thermal stability, etc. It can selectively kill some pathogenic bacteria, and can protect and promote the growth of probiotics in the body, thereby regulating the immune function of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K50/60A23K50/75A23K20/147C07K14/335
CPCA23K20/147A23K50/30A23K50/60A23K50/75C07K14/335
Inventor 樊倩周盛昌肖丽萍檀新珠黄彪汤建平仲伟迎李兵吴有林
Owner 江苏傲农生物科技有限公司
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