Preparation method of stable nanogel polyferose

Abstract

The invention discloses a preparation method of stable nanogel polyferose and belongs to the field of preparation of polyferose. According to the preparation method, a novel nanoscale gel microsphereis prepared based on specific binding of lambda-carrageenan iron and ferric ion, the polyferose nanogel microsphere prepared through a stirring-dripping method shows good dispersion stability and significantly reduces the cytotoxicity of the iron ions; meanwhile, surface hydrophobic modification is further performed on the gel microsphere to improve the storage stability of polyferose powder, anddue to hydrolytic stability of the gel microsphere for a gastrointestinal tract, a theoretical support and a technical guidance are provided for development of a novel iron reinforcing agent; and theprepared lambda-car-Fe3+ complex can isolate moisture and inhibit browning, thus the storage stability and the hydrolytic stability for the gastrointestinal tract are greatly improved, and the stablenanogel polyferose as a safe iron reinforcing agent has great application potentials.

Description

technical field

[0001] The invention belongs to the field of polysaccharide iron preparation, and in particular relates to a preparation method of stable nano-gel polysaccharide iron, more specifically a preparation method for improving the dispersibility and storage stability of polysaccharide iron. Background technique

[0002] Iron is one of the most important minerals for the human body and is the main element in the manufacture of hemoglobin and myohemoglobin. According to WHO reports, one quarter of children aged 4-14 and pregnant women suffer from anemia in the world, and most of the anemia is caused by iron deficiency. Iron deficiency anemia is a common global nutritional disease in modern society due to dietary iron deficiency, malabsorption, and excessive loss of iron. Oral iron supplementation is a common method of treatment and prevention of iron deficiency anemia. However, there are many disadvantages in commonly used oral iron supplements, such as easy to rea...

Examples

Embodiment 1

[0025] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0026] (2) The preparation concentration is 200 mM FeCl 3 mother liquor; a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and add a certain amount of water to make the final mass concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 1 mM; in order to prevent the hydrolysis of metal ions, the sample was adjusted to pH 2.3, the sample was stirred at 800 rpm for 24 h under continuous magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash off the free iron ions, vacuum freeze-dried to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

Embodiment 2

[0028] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0029] (2) The preparation concentration is 200 mM FeCl 3 mother liquor, a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and then add a certain amount of water to make the final concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 5 mM; in order to prevent the hydrolysis of metal ions, the pH of the sample was adjusted to 2.3, the sample was stirred at 800 rpm for 24 h under constant magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash away free iron ions, Vacuum freeze-drying to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

Embodiment 3

[0031] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0032] (2) The preparation concentration is 200 mM FeCl 3 mother liquor, a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and then add a certain amount of water to make the final concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 10 mM; in order to prevent the hydrolysis of metal ions, the pH of the sample was adjusted to 2.3, the sample was stirred at 800 rpm for 24 h under constant magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash off free iron ions, Vacuum freeze-drying to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

[0033] We observe that the λ-car-Fe prepared in this example 3+ Microscopic morphology and appearance properties ...

technical field

[0001] The invention belongs to the field of polysaccharide iron preparation, and in particular relates to a preparation method of stable nano-gel polysaccharide iron, more specifically a preparation method for improving the dispersibility and storage stability of polysaccharide iron. Background technique

[0002] Iron is one of the most important minerals for the human body and is the main element in the manufacture of hemoglobin and myohemoglobin. According to WHO reports, one quarter of children aged 4-14 and pregnant women suffer from anemia in the world, and most of the anemia is caused by iron deficiency. Iron deficiency anemia is a common global nutritional disease in modern society due to dietary iron deficiency, malabsorption, and excessive loss of iron. Oral iron supplementation is a common method of treatment and prevention of iron deficiency anemia. However, there are many disadvantages in commonly used oral iron supplements, such as easy to rea...

Examples

Embodiment 1

[0025] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0026] (2) The preparation concentration is 200 mM FeCl 3 mother liquor; a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and add a certain amount of water to make the final mass concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 1 mM; in order to prevent the hydrolysis of metal ions, the sample was adjusted to pH 2.3, the sample was stirred at 800 rpm for 24 h under continuous magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash off the free iron ions, vacuum freeze-dried to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

Embodiment 2

[0028] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0029] (2) The preparation concentration is 200 mM FeCl 3 mother liquor, a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and then add a certain amount of water to make the final concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 5 mM; in order to prevent the hydrolysis of metal ions, the pH of the sample was adjusted to 2.3, the sample was stirred at 800 rpm for 24 h under constant magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash away free iron ions, Vacuum freeze-drying to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

Embodiment 3

[0031] (1) The λ-carrageenan sample was dispersed in ultrapure water and stirred at 85 °C for 1 h to obtain a λ-carrageenan mother liquor with a mass concentration of 2.0%.

[0032] (2) The preparation concentration is 200 mM FeCl 3 mother liquor, a certain amount of FeCl 3 Drop the mother liquor into the λ-carrageenan mother liquor, and then add a certain amount of water to make the final concentration of the λ-carrageenan solution 1% (w / w), FeCl 3 The final concentration of the sample was 10 mM; in order to prevent the hydrolysis of metal ions, the pH of the sample was adjusted to 2.3, the sample was stirred at 800 rpm for 24 h under constant magnetic stirring, and then centrifuged at 1000 g for 15 min, washed three times with water to fully wash off free iron ions, Vacuum freeze-drying to obtain λ-car-Fe 3 + Gel microspheres are solid, save for future use.

[0033] We observe that the λ-car-Fe prepared in this example 3+ Microscopic morphology and appearance properties ...

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